Thymidine and Testosterone Incorporation by Bursal and Thymic Lymphocytes

Glick, Bruce; Schwarz, M. Roy

doi:10.3109/08820137509055767

Cited by 39 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This involved mincing the gland and expressing free cells through lens paper (Glick and Schwartz, 1975) prior to layering the resulting cell SUSpension over a double density ficoll gradient. The gradient consisted of a 1.083 g/mL solution on top of a 1.114 g/mL solution.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Immune cell proliferation in the Harderian gland: An avian model

Scott

Savage

1996

Microsc. Res. Tech.

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

“…Two different bioassays were used to determine proliferative activity of the supernatants. In both cases, cells from the bursa of Fabricius were used as the stimulator cells and were collected as described by Glick and Schwartz (1975). In the first bioassay the bursal cell concentration was adjusted to 4 x 10' cells per milliliter in 1 .…”

Section: Methodsmentioning

confidence: 99%

Immune cell proliferation in the Harderian gland: An avian model

Scott

Savage

1996

Microsc. Res. Tech.

View full text Add to dashboard Cite

“…The minced tissue was placed on lens pa per (5 cm2) and supported on a Maximov chamber. The paper was folded, H B SS added, and the cells were expressed by gentle pressing [16]. The cells were transfered to test tubes (4 CC) and later cen trifuged at 1,000 rpm for 10 min at 4 ?C .…”

Section: Ell Suspensionsmentioning

confidence: 99%

Effect of Complement Inactivated Rabbit Antichick Brain Serum on Thymic and Splenic Lymphocytes

Barnett¹,

Glick²

1979

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

The effects on in vitro exposure of chicken thymic-dependent lymphocytes to complement inactivated rabbit antichick brain serum (RACB) previously absorbed with normal serum (ns) and kidney (k) [RACB^{ns, k}] was investigated. Spleen lymphocytes which normally initiate a ‘graft vs. host’ reaction give rise to much more intense reactions when treated with (RACB)^{ns, k}. This may be due to increased antigenicity of the donor cell and/or stimulation of the donor T cell. Migration studies showed that chicken thymocytes are capable of in vitro migration which can be inhibited by exposure to (RACB)^{ns, k}. Thymocytes pretreated with (RACB)^{ns, k} were found to express factor(s) capable of inhibiting thymocyte migration. The correlation between lymphokines and increased splenomegaly was discussed.

show abstract

“…Water-soluble substances enter the embryo when these substances are applied to the air cell [14]. This method eliminates the tediousness of preparing eggs for intravenous injection and reduces the embryonic mortality which results, in part, from the injection procedure.…”

Section: Introductionmentioning

confidence: 99%

Avian Immune Capacity and Bone Marrow Cellularity after in ovo Treatment with Cyclophosphamide

Glick¹

1986

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Lymphoid tissue, immunity and erythropoiesis in bone marrow were studied in chickens which had received air cell applications of cyclophosphamide (Cy) between 16 and 18 days of their embryonic development. The bursae from the Cy-treated birds were reduced significantly in size, deficient in bursal follicles, and lacked lymphocytes. The agglutinin level of sheep red blood cells of birds treated with Cy (2 mg) as 16, 17 and 18-day embryos was significantly lower than controls. While these Cy-treated birds lacked IgG antibody to sheep red blood cells, about 50% of the Cy birds produced unspecific IgG. Cy treatment did not suppress the graft-versus-host response. Cy did not change the absolute number of lymphocytes, granulocytes or erythroid series of cells in the bone marrow but did eliminate plasma cells. Since some of the Cy-treated birds did not produce specific agglutinin but made Ig, one would have to conclude that the presence of the bursa was not obligatory for Ig synthesis, but that the bursal microenvironment may be a prerequisite for synthesis of specific antibody.

show abstract

Thymidine and Testosterone Incorporation by Bursal and Thymic Lymphocytes

Cited by 39 publications

References 17 publications

Immune cell proliferation in the Harderian gland: An avian model

Immune cell proliferation in the Harderian gland: An avian model

Effect of Complement Inactivated Rabbit Antichick Brain Serum on Thymic and Splenic Lymphocytes

Avian Immune Capacity and Bone Marrow Cellularity after in ovo Treatment with Cyclophosphamide

Contact Info

Product

Resources

About