Thrombogenic Effects of a Nonthrombin-Based Fibrin Sealant Compared With Thrombin-Based Fibrin Sealant on Microvenous Anastomoses in a Rat Model

Drake, David B.; Faulkner, Brent C.; Amiss, L. R.; Spotnitz, William D.; Morgan, Raymond F.

doi:10.1097/00000637-200045050-00009

Cited by 14 publications

(13 citation statements)

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“…These efforts included work in a variety of surgical disciplines including breast [9, 13, 14, 16, 17, 32, 36], cardiovascular [11, 26, 27, 30, 33], gastrointestinal [10], head and neck [22, 35], hepatic [12, 20], neuro- [29], orthopedic [21, 37, 40, 41], pediatric [12], noncardiac thoracic [25, 30, 31, 33, 34], and vascular surgery [15, 18, 19, 23, 24, 38, 39]. This body of work emphasized the evolving versatility of fibrin sealant because it is capable of causing blood to clot, creating a sealing barrier, and gluing tissues together.…”

Section: Introductionmentioning

confidence: 99%

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

2014

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Background. Fibrin sealant became the first modern era material approved as a hemostat in the United States in 1998. It is the only agent presently approved as a hemostat, sealant, and adhesive by the Food and Drug Administration (FDA). The product is now supplied as patches in addition to the original liquid formulations. Both laboratory and clinical uses of fibrin sealant continue to grow. The new literature on this material also continues to proliferate rapidly (approximately 200 papers/year). Methods. An overview of current fibrin sealant products and their approved uses and a comprehensive PubMed based review of the recent literature (February 2012, through March 2013) on the laboratory and clinical use of fibrin sealant are provided. Product information is organized into sections based on a classification system for commercially available materials. Publications are presented in sections based on both laboratory research and clinical topics are listed in order of decreasing frequency. Results. Fibrin sealant remains useful hemostat, sealant, and adhesive. New formulations and applications continue to be developed. Conclusions. This agent remains clinically important with the recent introduction of new commercially available products. Fibrin sealant has multiple new uses that should result in further improvements in patient care.

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Section: Introductionmentioning

confidence: 99%

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

2014

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“…19 The lack of comprehension of this fact may be responsible for these ''contradictory'' results reported in the literature. This was further confirmed by Drake et al 20 and Frost-Arner et al, 21 who demonstrated that the application of lower concentrations (500 IU/ml) of thrombin in conjunction with fibrinogen, did not increase thrombogenicity in epigastric free flap models.…”

Section: Discussionmentioning

confidence: 57%

“…This experiment was not the only one to test the fibrin glue in free flaps' microvascular anastomoses, however, it was the more realistic one. In previous studies, 1,3,10,18,20,21 after the flap elevation, the pedicle was ligated and the flap was repositioned in its own bed. Consequently, all the anastomoses were performed between perfectly matching donor and recipient vessels, and always in an end-to-end fashion.…”

Section: Discussionmentioning

confidence: 99%

Application of fibrin glue in microvascular anastomoses: Comparative analysis with the conventional suture technique using a free flap model

Cho

Júnior

2008

Microsurgery

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“…A thrombin-based preparation of fibrin sealant has recently been shown to have deleterious effects on microvascular anastomosis in an animal model [11] . Fibrin sealant constructed with a high concentration of bovine thrombin (1,000 IU/ml) was detrimental to microvascular patency when applied to the anastomosis in a rat free flap model [12] . For microvascular anastomosis, lower concentrations of thrombin or a sealant devoid of thrombin seem to be best for microvascular anastomotic patency.…”

Section: Discussionmentioning

confidence: 98%

A Modified Technique of Renal Artery Anastomosis in Rat Kidney Transplantation

2009

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Aims: To reduce warm ischemic time and avoid irreversible damage to the graft in rat kidney transplantation. Methods: After left nephrectomy, recipients were transplanted with syngeneic kidney grafts using microsurgical techniques. In control rats (n = 20), the renal artery anastomoses were performed with 8–9 interrupted sutures by the conventional technique. In experimental animals (n = 20), a modified anastomosis was performed using fewer (5–6) sutures and fibrin glue devoid of thrombin. Results: The number of sutures in the control group was 8.09 ± 0.35 while that in the experimental group was 5.65 ± 0.48 (p < 0.01). The warm ischemic time reduced from 29.7 ± 1.1 min in the control group to 23.9 ± 0.9 min in the experimental group (p < 0.01). These anastomoses maintained adequate patency rates and mechanical strength. Up to 21 days after operation, the graft survival rates in the experimental and control groups were 90 and 85%, respectively. Conclusions: Our modified technique for renal artery anastomosis significantly reduced the warm ischemic time in rat kidney transplantation. This technique would be a safe and reliable method for rat renal artery anastomosis as well as for other microarterial anastomoses, particularly for novice surgeons.

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Thrombogenic Effects of a Nonthrombin-Based Fibrin Sealant Compared With Thrombin-Based Fibrin Sealant on Microvenous Anastomoses in a Rat Model

Cited by 14 publications

References 0 publications

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

Fibrin Sealant: The Only Approved Hemostat, Sealant, and Adhesive—a Laboratory and Clinical Perspective

Application of fibrin glue in microvascular anastomoses: Comparative analysis with the conventional suture technique using a free flap model

A Modified Technique of Renal Artery Anastomosis in Rat Kidney Transplantation

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