Thromboelastometric and platelet responses to silk biomaterials

Abstract

Silkworm's silk is natural biopolymer with unique properties including mechanical robustness, all aqueous base processing and ease in fabrication into different multifunctional templates. Additionally, the nonmulberry silks have cell adhesion promoting tri-peptide (RGD) sequences, which make it an immensely potential platform for regenerative medicine. The compatibility of nonmulberry silk with human blood is still elusive; thereby, restricts its further application as implants. The present study, therefore, evaluate the haematocompatibility of silk biomaterials in terms of platelet interaction after exposure to nonmulberry silk of Antheraea mylitta using thromboelastometry (ROTEM). The mulberry silk of Bombyx mori and clinically used Uni-Graft W biomaterial serve as references. Shortened clotting time, clot formation times as well as enhanced clot strength indicate the platelet mediated activation of blood coagulation cascade by tested biomaterials; which is comparable to controls.

Functionalized PVA –silk blended nanofibrous mats promote diabetic wound healing via regulation of extracellular matrix and tissue remodelling

“…PVAAA and PVAPR showed significantly higher LDH activity (4.32 ± 0.72 and 4.04 ± 0.54 milliunits/ml, respectively) due to the presence of RGD sequences attracting more platelets, suggesting their superior haemostatic property ( p ≤ .01). Greater amount of platelet interaction was also observed with another NMSF Antheraea mylitta as compared with BMSF owing to RGD sequences in Antheraea mylitta protein supporting our results (Kundu, Schlimp, Nürnberger, Redl, & Kundu, ).…”

“…PVAAA and PVAPR showed significantly higher LDH activity (4.32 ± 0.72 and 4.04 ± 0.54 milliunits/ml, respectively) due to the presence of RGD sequences attracting more platelets, suggesting their superior haemostatic property (p ≤ .01). Greater amount of platelet interaction was also observed with another NMSF Antheraea mylitta as compared with BMSF owing to RGD sequences in Antheraea mylitta protein supporting our results(Kundu, Schlimp, Nürnberger, Redl, & Kundu, 2014).3.3 | Antibacterial and antibiofilm activity of LL-37 functionalized nanofibrous mats-In vitro and in vivo assayBacterial colonization and biofilm development are commonly observed in diabetic wounds, in response to high glucose environment(Mottola et al, 2016). Bioburden inflicted on chronic wounds, by the presence of polymicrobes, has been evidenced, particularly in the diabetic condition(Martin, Zenilman, & Lazarus, 2010).…”

Tethered‐liquid omniphobic surface coating reduces surface thrombogenicity, delays clot formation and decreases clot strength ex vivo

“…We compared two different LPs for the mobile surface layer: perfluorodecalin (PFD) and Fluorolube 63 (FL63). TEG is a viscoelastic clotting test that has been used to investigate the impact of various biomaterials at the blood/biomaterial interface in ex vivo blood preparations (10)(11)(12)(13). It has been identified as a useful screening tool to select promising biomaterial candidates for in vivo testing (14).…”

Maksi̇llofasi̇yal Cerrahi̇de Kullanilan Di̇ki̇ş Materyalleri̇: Derleme

“…DikiĢ materyalleriyle ilgili en eski çalıĢmalar Mısır'da M.Ö. 3000 yıllarında ketenin yaraların kapatılmasında 6 M.S. 175 yıllarında Roma gladyatörlerinin doktoru olan Galen, yaraların kapatılması için katgütten yararlanmıĢtır.…”