Functionalized
            <scp>PVA</scp>
            –silk blended nanofibrous mats promote diabetic wound healing via regulation of extracellular matrix and tissue remodelling

Chouhan, Dimple; Janani, G.; Chakraborty, Bijayashree; Nandi, Samit Kumar; Mandal, Biman B.

doi:10.1002/term.2581

Cited by 88 publications

(78 citation statements)

References 43 publications

(81 reference statements)

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“…However, sufficient vascularized granulation tissue was developed in SF‐treated wounds when compared to untreated. This depicts early recruitment of endothelial cells and angiogenic potential of SF gel owing to the vascularization capacity of both BmSF and AaSF proteins as also reported in previous studies …”

Section: Resultssupporting

confidence: 86%

“…BmSF, the mulberry silk variety is known for its wound healing properties and deposition of collagen fibers in various studies . In addition, AaSF possesses Arg cores along with RGD cell binding motifs which aid in faster wound healing owing to its cell‐material interactive properties . Thus, the combination of BmSF and AaSF in SF hydrogel resulted in accelerated healing with normal wound repair process.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, SF also possesses tunable mechanical properties to match the desired resilience and elasticity of native tissues . SF supports cell proliferation and extracellular matrix (ECM) deposition thus making direct contribution toward wound repair and tissue regeneration process . SF‐based constructs like porous scaffolds, nanofibrous mats, and thin films have been highly explored for treating acute wounds .…”

Section: Introductionmentioning

confidence: 99%

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In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Chouhan

Lohe

Samudrala

2018

Adv Healthcare Materials

Self Cite

166

126

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Full‐thickness skin wounds, associated with deep burns or chronic wounds pose a major clinical problem. Herein, the development of in situ forming hydrogel using a natural silk fibroin (SF) biomaterial for treating burn wounds is reported. Blends of SF solutions isolated from Bombyx mori and Antheraea assama show inherent self‐assembly between silk proteins and lead to irreversible gelation at body temperature. Investigation of the gelation mechanism reveals crosslinking due to formation of β‐sheet structures as examined by X‐ray diffraction and Fourier transform infrared spectroscopy. The SF hydrogel supports proliferation of primary human dermal fibroblasts and migration of keratinocytes comparable to collagen gel (Col) as examined under in vitro conditions. The SF hydrogel also provides an instructive and supportive matrix to the full‐thickness third‐degree burn wounds in vivo. A 3‐week comparative study with Col indicates that SF hydrogel not only promotes wound healing but also shows transitions from inflammation to proliferation stage as observed through the expression of TNF‐α and CD163 genes. Further, deposition and remodeling of collagen type I and III fibers suggests an enhanced overall tissue regeneration. Comparable results with Col demonstrate the SF hydrogel as an effective and inexpensive formulation toward a potential therapeutic approach for burn wound treatment.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Chouhan

Lohe

Samudrala

2018

Adv Healthcare Materials

Self Cite

166

126

View full text Add to dashboard Cite

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“…The use of gelatin nanofibers having enough tensile strength for fabricating nonwoven mats has received attention recently for use for antimicrobial applications [45][46][47][48][49]. Successful spinning of minimum diameter nanofibers results in the surface areas of these nanofibers being increased in addition to their light weight.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning process parameters optimization for biofunctional curcumin/gelatin nanofibers

Kanu

Gupta

Vates

et al. 2020

Mater. Res. Express

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Electrospinning has received wide attention for the preparation of uniform diameter nanofibers (ranging from 5 nm to several hundred nanometers) in films with random as well as aligned fashions of the fibers of various materials for use in biomedical applications. Electrospinning research has provided an in-depth understanding of the preparation of light weight, ultrathin, porous, biofunctional curcumin/gelatin nanofibers having applications in wound dressing, drug release, tissue engineering, etc. In the first half of this article, prior research on electrospun curcumin/gelatin nanofibers is reviewed in depth with nanofibers being desired due to their low diameters since these would have then large surface area to volume ratio and enough film porosity as well as improved mechanical (tensile) strength so that when prepared as mats these nanofibers (having high biocompatibility) could be used for sustained release of curcumin and oxygen to wounds during healing. The synthesis of ultrathin nanofibers (having minimum average diameter) is not a simple task unless numerical investigation is carefully done in the first half of this research article. The authors research described here examined the effects of critical process parameters (in the second half of the paper) such as distance between the spinneret and collector, flow rate, voltage and solution viscosity, on the preparation of uniform and ultrathin nanofibers using scanning electron microscopy (SEM) for characterization of the nanofibers. A 2 k factorial design of experiment was found to be a suitable and efficient technique to optimize the critical process parameters used in the preparation of the biofunctional nanofibers with the purpose of having applications in the treatment of problematic wounds such as diabetic chronic ulcers. After parametric investigation, the distance, flow rate and voltage when taken together, were found to have the most significant contributions to the preparation of minimum diameter nanofibers. The primary objective of this research was fulfilled with the development of ultrathin curcumin/gelatin nanofibers having a 181 nm (181±66 nm) average diameter using the optimized setting of a solution having 1.5% gelatin, and 1% curcumin in 10 ml of 98% concentrated formic acid, with the electrospining unit having a voltage of 10 KV, distance from the spinneret to collector drum of 15 cm, flow rate of 0.1 ml h −1 , viscosity of 65 cP and drum collector speed of 1000 rpm. However, the lowest average diameter of nanofiber was measured around 147 nm (147±34 nm) which was prepared at a higher voltage, such as 15 KV (at 10 cm distance, 0.15 ml h −1 flow rate and 65 cP viscosity) using the solution. The design of this research paper is based on the view that merely optimization of biofunctional nanofibers may not fully satisfy researchers/ engineers unless they are also provided with sufficient information about (a) the entire electrospinning mechanism (numerical investigations of the mechanism) to have better control over preparation of ultr...

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“…Here, the synergistic activity of LL37 and serpin A1 enhanced the nanoparticles antibacterial activity against Staphylococcus aureus ( S. aureus ) and E. coli above the LL37 or serpin A1 alone . More recently, LL37 was functionalized onto PVA/silk fibroin nanofibrous mats by solution blending, and revealed a great efficiency in killing skin bacteria, such as Staphylococcus epidermidis ( S. epidermidis ), and preventing biofilm formation . To the authors' knowledge, functionalization of LL37 has not yet been attempted on the surface of PVA/CA films for prospective applications in wound‐healing.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial action and clotting time of thin, hydrated poly(vinyl alcohol)/cellulose acetate films functionalized with LL37 for prospective wound‐healing applications

Felgueiras

Teixeira

Tavares

et al. 2019

J of Applied Polymer Sci

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Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling, and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈75% inhibition for S. aureus, ≈85% for S. epidermidis, and ≈60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.

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Functionalized PVA –silk blended nanofibrous mats promote diabetic wound healing via regulation of extracellular matrix and tissue remodelling

Cited by 88 publications

References 43 publications

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Electrospinning process parameters optimization for biofunctional curcumin/gelatin nanofibers

Antimicrobial action and clotting time of thin, hydrated poly(vinyl alcohol)/cellulose acetate films functionalized with LL37 for prospective wound‐healing applications

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