Thrombin immobilized polydopamine–diatom biosilica for effective hemorrhage control

Mu, Yuzhi; Fu, Yangmu; Li, Jing; Shao, Kai; Pang, Jianhui; Su, Chang; Cai, Yibo; Sun, Xiaoyang; Cong, Xin; Chen, Xiguang; Feng, Chao

doi:10.1039/d0bm02116d

Cited by 26 publications

(22 citation statements)

References 51 publications

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“…In 2021, Mu et al immobilized thrombin on the surface of polydopamine-coated diatom biosilica. This composite hemostatic material exhibited an effective clotting time with a low concentration (66 s at 5 mg mL −1 ), which was approximately 8% of the clotting time of the control group [42].…”

Section: Surface Modification Of Diatom Frustules Benefits Hemostatic...mentioning

confidence: 95%

Study on the Hemostasis Characteristics of Biomaterial Frustules Obtained from Diatom Navicula australoshetlandica sp.

Luo

Shen

et al. 2021

Materials

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Diatoms, known as photosynthetic unicellular algae, can produce natural biosilica frustules that exhibit great biocompatibility, superhydrophilicity, and superhemophilicity. In our study, the diatom Navicula australoshetlandica sp. was isolated from aquaculture wastewater and pretreated to obtain frustules so as to explore their hemostasis characteristics. A special “porous web” (6–8 nm) substructure in the ordered nanopores (165–350 nm) of boat-shaped diatom frustule was observed in Navicula australoshetlandica sp. using SEM and TEM analysis. Moreover, X-ray, N2 adsorption–desorption isotherms, and BET analysis showed that the diatom frustule is a mesoporous material with a surface area of 401.45 m2 g−1 amorphous silica. FTIR analysis showed that Navicula australoshetlandica sp. frustules possessed abundant OH functional groups. A low hemolysis ratio was observed for 1–5 mg mL−1 diatom frustules that did not exceed 1.55 ± 0.06%, which indicates favorable hemocompatibility. The diatom frustules exhibited the shortest clotting time (134.99 ± 7.00 s) with a hemostasis material/blood (mg/μL) ratio of 1:100, which is 1.83 times (112.32 s) shorter than that of chitosan. The activated partial thromboplastin time (aPTT) of diatom frustule was also 44.53 s shorter than the control. Our results demonstrate the potential of Navicula australoshetlandica sp. diatom frustules to be used as medical hemostasis material.

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Section: Surface Modification Of Diatom Frustules Benefits Hemostatic...mentioning

confidence: 95%

Study on the Hemostasis Characteristics of Biomaterial Frustules Obtained from Diatom Navicula australoshetlandica sp.

Luo

Shen

et al. 2021

Materials

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“…The hierarchical porous structure of DBs gives it ultrahigh porosity and huge surface areas, which has already been used in the fields of drug delivery, catalysis, adsorbents, electrode materials, etc. [24][25][26][27][28][29][30] Our previous research showed that diatom biosilica based materials have the advantages of high efficiency, being rapid and safe and having a simple preparation process, and is expected to be a rapid haemostatic agent. 31,32 It is noteworthy that the elegant microto nano-scale porosity characteristics of DBs through millions of years of evolution provide living diatoms with extremely high solar energy harvesting efficiency for adapting to low light conditions underwater, which reveal their promising applications in the photothermal field as well.…”

Section: Introductionmentioning

confidence: 99%

Copper deposited diatom-biosilica with enhanced photothermal and photodynamic performance for infected wound therapy

Cong

Qin

et al. 2022

New J. Chem.

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“…The size of DBs varies basically from 5 to 9 μm, and rich attachment sites were provided for Cu metal mineralization and Ce nanoparticles. The organics in the diatom could be removed by a mixture of H 2 SO 4 and H 2 O 2 solution at 70 °C, leaving the inorganic residues composed of highly pure amorphous silica as DBs. − The DBs exhibited a hierarchical porous structure with various pore sizes: the primary sector pore was ∼800 nm in length; the secondary circular pore was 30–50 nm in diameter; the tertiary circular pore was 1–2 nm in diameter. The doping of Cu into DBs was realized by a hydrothermal reaction .…”

Section: Resultsmentioning

confidence: 99%

Reversible Regulation of the Reactive Oxygen Species Level Using a Semiconductor Heterojunction

Yang

Shao

et al. 2022

ACS Appl. Mater. Interfaces

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Here, we proposed a novel solution for reversible regulation of the reactive oxygen species (ROS) level using a semiconductor heterojunction. Two metal-based ROS scavengers containing n-type CeO2 nanoparticles and n-type Cu-doped diatom biosilica (Cu-DBs) were integrated by a hydrothermal method to form a typical n–n semiconductor heterojunction (Ce/Cu-DBs). Unlike the control of the ROS level by a single ROS scavenger or ROS-generating agent, Ce/Cu-DBs could quickly eliminate ROS by cascade catalytic reaction, which readily switched to ROS generation through a near-infrared (NIR)-triggered photocatalytic effect. This NIR mediated ROS regulation system provided a noninvasive strategy for reversible control of the ROS level in vitro and in vivo. The Ce/Cu-DBs could relieve cellular oxidative stress by clearing local excessive ROS while inhibiting bacterial growth by increasing ROS levels under NIR radiation. Benefiting from the reversible regulatory effect of Ce/Cu-DBs, programmable healing of infected wounds was realized via on-demand anti-infection and inflammation reduction. This work provided a general method with highly spatiotemporal resolution to a remote and sustainable control ROS level, which had great potential for the biomedical field and regulation of chemical reactions.

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Thrombin immobilized polydopamine–diatom biosilica for effective hemorrhage control

Abstract: In this study, the efficient composite hemostatic material (DA-diatom-T) was prepared, using polydopamine layer as a linker to immobilize thrombin on the surface of diatom biosilica. DA-diatom-T retained the porous...

Cited by 26 publications

References 51 publications

Study on the Hemostasis Characteristics of Biomaterial Frustules Obtained from Diatom Navicula australoshetlandica sp.

Study on the Hemostasis Characteristics of Biomaterial Frustules Obtained from Diatom Navicula australoshetlandica sp.

Copper deposited diatom-biosilica with enhanced photothermal and photodynamic performance for infected wound therapy

Reversible Regulation of the Reactive Oxygen Species Level Using a Semiconductor Heterojunction

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