Three water soluble polysaccharides with anti-inflammatory activities from Selaginella uncinata (Desv.) Spring

Hui, Haochen; Gao, Meng; Zhao, Xin; Yin, Lianhong; Xu, Lina; Li, Lili; Peng, Jinyong

doi:10.1016/j.ijbiomac.2022.09.287

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…As a result, the relative molar masses of Rha and Ara obtained through methylation exhibit slight variations from the monosaccharide composition results [16]. It has been reported that galactose in polysaccharides might be associated with anti-inflammatory effects [17], and a significant amount of galactose was found in RLPa-2.…”

Section: Methylation Analysis Of Rlpa-2mentioning

confidence: 90%

Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata

Peng,

Gu,

Mao

et al. 2024

IJMS

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RLPa-2 (Mw 15.6 kDa) is a polysaccharide isolated from Rosa laevigata Michx. It consists of arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), xylose (Xyl), and galacturonic acid (Gal-UA) with a molar ratio of 1.00:0.91:0.39:0.34:0.25:0.20. Structural characterization was performed by methylation and NMR analysis, which indicated that RLPa-2 might comprise →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, α-L-Araf-(1→, →2,4)-α-D-Glcp-(1→, β-D-Xylp, and α-L-Rhap. In addition, the bioactivity of RLPa-2 was assessed through an in vitro macrophage polarization assay. Compared to positive controls, there was a significant decrease in the expression of M1 macrophage markers (CD80, CD86) and p-STAT3/STAT3 protein. Additionally, there was a down-regulation in the production of pro-inflammatory mediators (NO, IL-6, TNF-α), indicating that M1 macrophage polarization induced with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) stimulation could be inhibited by RLPa-2. These findings demonstrate that the RLPa-2 might be considered as a potential anti-inflammatory drug to reduce inflammation.

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Section: Methylation Analysis Of Rlpa-2mentioning

confidence: 90%

Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata

Peng,

Gu,

Mao

et al. 2024

IJMS

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“…L-Ara synthesis in plants and green algae L-Ara is widely found not only in seed plants but also in ferns, mosses, and liverworts (Bohlender et al, 2022;Happ & Classen, 2019;Hui et al, 2022;Mueller et al, 2023). However, it has not been clear when during evolution the dual pathway for UDP-L-Ara was acquired.…”

Section: Other Factors Affecting the Amount Of Cell Wall L-aramentioning

confidence: 99%

Cytosolic UDP‐L‐arabinose synthesis by bifunctional UDP‐glucose 4‐epimerases in Arabidopsis

Umezawa,

Matsumoto,

Handa

et al. 2024

The Plant Journal

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SUMMARYL‐Arabinose (L‐Ara) is a plant‐specific sugar found in cell wall polysaccharides, proteoglycans, glycoproteins, and small glycoconjugates, which play physiologically important roles in cell proliferation and other essential cellular processes. L‐Ara is synthesized as UDP‐L‐arabinose (UDP‐L‐Ara) from UDP‐xylose (UDP‐Xyl) by UDP‐Xyl 4‐epimerases (UXEs), a type of de novo synthesis of L‐Ara unique to plants. In Arabidopsis, the Golgi‐localized UXE AtMUR4 is the main contributor to UDP‐L‐Ara synthesis. However, cytosolic bifunctional UDP‐glucose 4‐epimerases (UGEs) with UXE activity, AtUGE1, and AtUGE3 also catalyze this reaction. For the present study, we first examined the physiological importance of bifunctional UGEs in Arabidopsis. The uge1 and uge3 mutants enhanced the dwarf phenotype of mur4 and further reduced the L‐Ara content in cell walls, suggesting that bifunctional UGEs contribute to UDP‐L‐Ara synthesis. Through the introduction of point mutations exchanging corresponding amino acid residues between AtUGE1 with high UXE activity and AtUGE2 with low UXE activity, two mutations that increase relative UXE activity of AtUGE2 were identified. The crystal structures of AtUGE2 in complex forms with NAD+ and NAD+/UDP revealed that the UDP‐binding domain of AtUGE2 has a more closed conformation and smaller sugar‐binding site than bacterial and mammalian UGEs, suggesting that plant UGEs have the appropriate size and shape for binding UDP‐Xyl and UDP‐L‐Ara to exhibit UXE activity. The presented results suggest that the capacity for cytosolic synthesis of UDP‐L‐Ara was acquired by the small sugar‐binding site and several mutations of UGEs, enabling diversified utilization of L‐Ara in seed plants.

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Exopolysaccharide from Bifidobacterium breve alleviate dextran sulfate sodium-induced colitis in mice via inhibiting oxidative stress and regulating intestinal flora

Niu,

Guo,

Cai

et al. 2023

Food Bioscience

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Three water soluble polysaccharides with anti-inflammatory activities from Selaginella uncinata (Desv.) Spring

Cited by 6 publications

References 45 publications

Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata

Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata

Cytosolic UDP‐L‐arabinose synthesis by bifunctional UDP‐glucose 4‐epimerases in Arabidopsis

Exopolysaccharide from Bifidobacterium breve alleviate dextran sulfate sodium-induced colitis in mice via inhibiting oxidative stress and regulating intestinal flora

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