Three synthetic biology applications and their paths to impact in Australia: Cane toads, bacteriophages, and biomining microbes

Abstract

Synthetic biology [synbio] applications have the potential to assist in addressing significant global health and environmental challenges. Australian research institutes are investing in formative research to develop synbio technologies capable of meeting these challenges. Alongside the laboratory research, investigating the broader social, institutional, and ethical considerations that synbio presents has been a priority. We conducted targeted qualitative research to uncover the barriers and opportunities for a range of multisectoral stakeholders identified as potential end-users of the science under development. The research provides insights into the research implementation environment for three synthetic biology applications: (1) gene editing cane toads (Rhinella marina) to reduce their environmental impact; (2) engineering bacteriophages to combat antimicrobial resistance in humans; and (3) engineering microbes to improve biomining efficiency in the mining industry. In-depth interviews (N = 23) with government, research and civil society representatives revealed key challenges in the impact pathway for each application. The strongest themes uncovered during interviews related to perceived negative public attitudes towards genetic technologies, a lack of investment in critical research infrastructure, unclear regulatory pathways and the presence of a strong social and environmental imperative underpinning technology development. These findings reveal specific entry points for further engagement with the most immediate end-users of synbio. Separate from research on public attitudes to synbio, the cases highlight the various hurdles to achieving research impact, according to experts who will likely use, approve or invest in these applications in the future. The themes uncovered inform avenues for strengthening engagement and research coordination in Australia and elsewhere.

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

“…Biomining includes processes like biosorption (i.e., the adsorption of dissolved metals to the surface of cells) and bioprecipitation (i.e., altering the redox state of dissolved metals to decrease their solubility in water), which have been extensively reported in the literature as alternatives (Figure 1A ). While some researchers have focused on leveraging microbes’ natural capabilities for biomining, others have explored the expansion the microbes’ capabilities through the latest advances in synthetic biology [ 2 , 3 , 4 ].…”

Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

“…1A). While some researchers have focused on leveraging microbes' natural capabilities for biomining, others have explored the expansion the microbes' capabilities through the latest advances in synthetic biology (2)(3)(4).…”