Engineered nickel bioaccumulation in <i>Escherichia coli</i> by NikABCDE transporter and metallothionein overexpression

Diep, Patrick; Shen, Haiwei; Wiesner, J. A.; Mykytczuk, Nadia; Papangelakis, Vladimiros G.; Yakunin, Alexander F.; Mahadevan, Radhakrishnan

doi:10.1002/elsc.202200133

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…We also identified the transcriptional regulator cueR, which enhances the expression of the also present copA, whose function is to transport Cu (I) from the cytosol to the periplasm, where it is reduced to Cu (II) (a less toxic form) by CueO [75]. We also identified nikABCDE operon, which encodes an Ni import system [76], and rcnA, which is translated into a membrane-associated protein whose presence has been related to an increase in resistance to Co and Ni. Lastly, focusing on the mechanisms of tolerance to Zn, we found zntA and zntR, which coded for a type-P ATPase that was predicted as the first Zn-specific transporter, responsible for the release of Zn ions out of the cell.…”

Section: Heavy Metal Tolerance Assaymentioning

confidence: 99%

Dark Fermentation in the Dark Biosphere: The Case of Citrobacter sp. T1.2D-12

Gallego-Rodríguez,

Martínez-Bonilla,

Rodríguez

et al. 2023

Fermentation

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Microbial diversity that thrives in the deep subsurface remains largely unknown. In this work, we present the characterization of Citrobacter sp. T1.2D-1, isolated from a 63.6 m-deep core sample extracted from the deep subsurface of the Iberian Pyrite Belt (IPB). A genomic analysis was performed to identify genes that could be ecologically significant in the IPB. We identified all the genes that encoded the formate–hydrogen lyase and hydrogenase-2 complexes, related to hydrogen production, as well as those involved in glycerol fermentation. This is particularly relevant as some of the substrates and byproducts of this process are of industrial interest. Additionally, we conducted a phylogenomic study, which led us to conclude that our isolate was classified within the Citrobacter telavivensis species. Experimentally, we verified the strain’s ability to produce hydrogen from glucose and glycerol and, thus, of performing dark fermentation. Moreover, we assessed the activity of the nitrate and tetrathionate reductase complexes and the isolate’s ability to tolerate high concentrations of heavy metals, especially Zn. These results suggest that C. telavivensis T1.2D-1 can play a role in the carbon, hydrogen, iron, nitrogen, and sulfur cycles that occur in the deep subsurface of the IPB, making it a candidate worthy of further study for possible biotechnological applications.

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Section: Heavy Metal Tolerance Assaymentioning

confidence: 99%

Dark Fermentation in the Dark Biosphere: The Case of Citrobacter sp. T1.2D-12

Gallego-Rodríguez,

Martínez-Bonilla,

Rodríguez

et al. 2023

Fermentation

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“…Three servers detected the presence of multiple genes that might confer tolerance to heavy metals like magnesium, cobalt, cadmium, zinc, iron, uoride and arsenic.inside the cell and its bioaccumulation(Rodionov et al, 2006;Diep et al, 2022). DFAST could detect a nickel ABC transporter-ATP binding protein.…”

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confidence: 99%

Genome sequencing, annotation and application of a strain of Microbacterium paraoxydans – a heavy metal hypertolerant and plant growth promoting bacterium

Mandal,

Das,

Adhikari

et al. 2024

Preprint

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A heavy metal hypertolerant plant growth promoting bacterium was isolated from arsenic contaminated garden soil of Bhagobangola I block (Murshidabad district). Metagenomic classification identified the bacterial isolate as a member of genus Microbacterium. Taxonomic assessment showed maximum average nucleotide identity (89.99%) with Microbacterium paraoxydans strain DSM 15019. Prokaryotic genome annotation was performed by Prokka, DFAST and RAST. The entire genome consisted of 3365911 bases with 69.90% GC-content. Prokka detected 3216 coding sequences (1461 hypothetical sequences), 3283 genes, 10 miscellaneous RNA, 3 rRNA, 53 tRNA and 1 tmRNA. DFAST detected 3257 coding sequences (1217 hypothetical sequences), 3 rRNA and 53 tRNA. Both Prokka and DFAST failed to detect any CRISPR sequence. RAST could detect 3285 coding sequences and 49 RNAs in the genome. Only 25% (821) of these sequences fell within the subsystem of RAST, which included 789 coding sequences as non-hypothetical and 32 sequences as hypothetical. Genes and Gene clusters responsible for arsenic resistance (arsR, arsB, arsC, acr1, acr2, acr3), other heavy metal (Copper, Manganese, Zinc, etc.) tolerance and plant growth promotion (Auxin biosynthesis, Siderophore mediated iron acquisition, Phosphate and polyphosphate metabolism, Trehalose biosynthesis, etc.) could also be identified in the Microbacterium paraoxydans genome. The heavy metal (arsenic) hypertolerance, bioremediation potential and plant growth promoting nature of the bacterium were confirmed by plate assay, SDDC assay and pot experiments, respectively. The aforementioned traits point towards the probable application of the bacterium as a bioremediation tool and biofertilizer, for reduction of arsenic toxicity and promotion of plant growth.

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Metallothionein: A Comprehensive Review of Its Classification, Structure, Biological Functions, and Applications

Yang,

Roshani,

Gao

et al. 2024

Antioxidants

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Metallothionein is a cysteine-rich protein with a high metal content that is widely found in nature. In addition to heavy metal detoxification, metallothionein is well known as a potent antioxidant. The high sulfhydryl content of metallothionein confers excellent antioxidant activity, enabling it to effectively scavenge free radicals and mitigate oxidative stress damage. In addition, metallothionein can play a neuroprotective role by alleviating oxidative damage in nerve cells, have an anticancer effect by enhancing the ability of normal cells to resist unfavorable conditions through its antioxidant function, and reduce inflammation by scavenging reactive oxygen species. Due to its diverse biological functions, metallothionein has a broad potential for application in alleviating environmental heavy metal pollution, predicting and diagnosing diseases, and developing skin care products and health foods. This review summarizes the recent advances in the classification, structure, biological functions, and applications of metallothionein, focusing on its powerful antioxidant effects and related functions.

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Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression

Cited by 3 publications

References 30 publications

Dark Fermentation in the Dark Biosphere: The Case of Citrobacter sp. T1.2D-12

Dark Fermentation in the Dark Biosphere: The Case of Citrobacter sp. T1.2D-12

Genome sequencing, annotation and application of a strain of Microbacterium paraoxydans – a heavy metal hypertolerant and plant growth promoting bacterium

Metallothionein: A Comprehensive Review of Its Classification, Structure, Biological Functions, and Applications

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