Three‐solvent extracting method comprehensively evaluates phenolics profile and antioxidant activities of Tartary buckwheat

Abstract

A three‐solvent extracting method, which consist of 80% acetone, ethyl acetate, and 70% ethanol extracting procedures, was adopted to evaluate phenolics profile and antioxidant activities of Tartary buckwheat. Phenolics profile of Tartary buckwheat was determined using HPLC. Gallic acid, chlorogenic acid, and 2,3,4‐trihydroxybenzoic acid were detected in 80% acetone extracts, 2,3,4‐trihydroxybenzoic acid was detected in ethyl acetate extracts, rutin and quercetin were detected in 70% ethanol extracts, rutin was the dominant phenolic compound, and the contents ranged from 1,902.0 to 2,402.8 mg/100 g DW. Antioxidant activities of Tartary buckwheat were determined using DPPH, ABTS, and FRAP assays, 70% ethanol extracts showed a higher antioxidant activity than 80% acetone and ethyl acetate, respectively. These results suggested that the three‐solvent extracting method is more comprehensive than independent one in evaluating phenolics profile and antioxidant activities of Tartary buckwheat. Practical applications The three‐solvent extracting method can be used to evaluate functional properties of Tartary buckwheat.

Nutrient composition, free radical scavenging activities and polyphenol contents of seabuckthorn (Hippophae tibetana) Western Himalayas, India

“…(2013) and Chen et al. (2021) that organic solvents might be suitable for the extraction of biochemical constituents from plants due to their diverse polarity. The present outcomes reported that moderate organic solvents are best for maximum extraction of bioactive constituents.…”

“…In the study, acetone and methanol are aqueous moderate polarity solvents, and more efficient than less polar solvents for extraction of bioactive constituents because most of the biochemical constituents are easily dissolved in solvents or water (Bahukhandi et al., 2020). Likewise, a few studies indicated that moderate polarity solvents have the capabilities to induce partial hydrolysis of conjugates/nonconjugated interactions, and extract out the highest chemical constitutes from insoluble complex (Chen et al., 2021; Nguyen et al., 2015; Pintac et al., 2018). Few researchers suggested that efficient extraction of bioactive constituents has required the use of suitable solvents with diverse polarities, solubility because no one single solvent can extract all the diverse bioactive constituents.…”

Functional components profile and glycemic index of kidney beans

“…The supernatant was evaporated to 5 mL using a rotary evaporator at 45 ° C, and the volume was then constituted to 10 mL with deionized water. Lastly, the extract was stored in a refrigerator at −20 ° C for subsequent experiments ( 20 ). Each kidney bean powder was extracted in triplicate.…”

Tartary Buckwheat in Human Nutrition

“…Chen et al [ 73 ] suggested a new three-solvent mix for efficient and comprehensive extraction of phenolics from Tartary buckwheat: acetone, ethyl acetate, and ethanol. This method is valuable for evaluation of the Tartary buckwheat functional properties.…”

“…Li et al [72] reported that the resveratrol in Tartary buckwheat bran does not show any detectable resveratrol in a bound form. Chen et al [73] suggested a new three-solvent mix for efficient and comprehensive extraction of phenolics from Tartary buckwheat: acetone, ethyl acetate, and ethanol. This method is valuable for evaluation of the Tartary buckwheat functional properties.…”