Three populations of mouse lymph node dendritic cells with different origins and dynamics

Salomon, Benoı̂t L.; Cohen, J.L.; Bruel, S.; Klatzmann, David

doi:10.1016/s0165-2478(97)85809-9

Cited by 57 publications

(79 citation statements)

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“…However, this is probably not the case as we did not detect bright FITC ϩ cells in the draining lymph nodes of WT C57BL/6 mice and RAG Ϫ/Ϫ mice 30 min or even 1 h after cutaneous FITC application. Others have also suggested that bright FITC ϩ DCs found in the draining lymph nodes after FITC painting are indeed derived from epidermal DCs (2,35). Additional evidence comes from our data obtained with migratory cells from skin explants of WT C57BL/6 and RAG Ϫ/Ϫ mice.…”

Section: Discussionsupporting

confidence: 71%

Langerhans Cells That Have Matured In Vivo in the Absence of T Cells Are Fully Capable of Inducing a Helper CD4 as Well as a Cytotoxic CD8 Response

Creus

Beneden

Taghon

et al. 2000

The Journal of Immunology

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Langerhans cells (LCs) are immature dendritic cells (DCs) present in the skin epithelium. Upon Ag exposure, they migrate to the draining lymph nodes where they mature into potent stimulators of naive T cells. The aim of this study was to investigate the influence of T cells on LC migration and maturation. Therefore, the in vivo migration and maturation of LCs after sensitization with the hapten FITC was compared between C57BL/6 or BALB/c mice used as positive controls, and recombination activating gene (RAG) 1 knockout (−/−) mice or SCID mice used as T cell-deficient mice. Phenotypically, there was no difference between migrated LCs from RAG1−/− or SCID mice vs normal C57BL/6 or BALB/c mice: both populations of FITC+ cells had a dendritic morphology and a mature phenotype as they expressed high levels of MHC class II molecules and costimulatory molecules CD80, CD86, and CD54. Sorted migrated LCs of RAG1−/− or SCID mice were efficient stimulators of allogeneic T cells and Ag-specific CD4+ T cells. The same results were found if migrated LCs were fixed instead of irradiated, excluding the possibility that LCs derived from RAG1−/− or SCID mice would mature in the presence of T cells during the stimulation tests. Importantly, fixed migrated LCs of RAG1−/− mice were also efficient stimulators of cytotoxic CD8+ T cells. These data suggest that T cells are not required for full maturation of LCs.

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Section: Discussionsupporting

confidence: 71%

Langerhans Cells That Have Matured In Vivo in the Absence of T Cells Are Fully Capable of Inducing a Helper CD4 as Well as a Cytotoxic CD8 Response

Creus

Beneden

Taghon

et al. 2000

The Journal of Immunology

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“…Based on this, three distinct DC subsets from the lymph nodes of naive CBA/J mice can be identified by flow cytometry, myeloid DC (MDC, DEC205 high FSC high CD8␣ low CD11b ϩ ), lymphoid DC (LDC, DEC205 high FSC high CD8␣ high CD11b low ), and Langerhans cells (DEC205 low FSC high CD8␣ Ϫ CD11b low ). These definitions are in keeping with the observations described by others (9,11) and were used in the investigations presented here for designating, MDC, LDC, and Langerhans cells.…”

Section: Identification Of Three DC Subsets Within the Lymph Node Fscmentioning

confidence: 68%

“…Although both populations can induce CD4 ϩ T cell proliferation in vitro, MDC are better inducers of CD4 ϩ T cell proliferation (7). An additional DC subset that is efficient at stimulating cell-mediated immune (CMI) responses is Langerhans cells, which are found in lymph nodes, but not spleens, of naive mice (11,12). In contrast, LDC from the spleen play a role in maintenance of peripheral tolerance through induction of apoptosis in activated, Fas-expressing CD4 ϩ T cells (7).…”

Section: S Ince the First Description Of Dendritic Cells (Dc) 3 In 1973mentioning

confidence: 99%

“…The cell suspensions were washed and resuspended in HBSS/5% FCS. This method of DC isolation was selected because the procedure extracts all subsets of DC (9,11). Furthermore, we avoided culture steps that might change the DC surface to a more activated phenotype.…”

Section: Preparation Of Single-cell Suspensionsmentioning

confidence: 99%

“…2E). Therefore, based on cell surface staining patterns and the large size of the cells, the cell subsets, DEC205 high FSC high CD8␣ low CD11b ϩ , DEC205 high FSC high CD8␣ high CD11b low , and DEC205 low FSC high CD8␣ Ϫ CD11b low , were considered DC (9,11,30). Based on this, three distinct DC subsets from the lymph nodes of naive CBA/J mice can be identified by flow cytometry, myeloid DC (MDC, DEC205 high FSC high CD8␣ low CD11b ϩ ), lymphoid DC (LDC, DEC205 high FSC high CD8␣ high CD11b low ), and Langerhans cells (DEC205 low FSC high CD8␣ Ϫ CD11b low ).…”

Section: Identification Of Three DC Subsets Within the Lymph Node Fscmentioning

confidence: 99%

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Dendritic Cells in the Induction of Protective and Nonprotective Anticryptococcal Cell-Mediated Immune Responses

Bauman

Nichols

Murphy

2000

The Journal of Immunology

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Dendritic cells (DC) can be divided into three subsets, Langerhans cells, myeloid DC (MDC), and lymphoid DC (LDC), based upon phenotypic and functional differences. We hypothesized that different DC subsets are associated with the development of protective vs nonprotective cell-mediated immune (CMI) responses against the fungal pathogen, Cryptococcus neoformans. To test this, mice were immunized with protective and/or nonprotective immunogens, and DC subsets in draining lymph nodes were assessed by flow cytometry. The protective immunogen (cryptococcal culture filtrate Ag-CFA), in contrast to the nonprotective immunogen (heat-killed cryptococci-CFA), the nonprotective immunogen mixed with the protective immunogen (cryptococcal culture filtrate Ag + heat-killed cryptococci-CFA), or controls, stimulated significant increases in total leukocytes, Langerhans cells, MDC, LDC, and activated CD4+ T cells in draining lymph nodes. The protective immune response resulted in significantly increased levels of anticryptococcal delayed-type hypersensitivity reactivity and activated CD4+ T cells at the delayed-type hypersensitivity reaction site. Draining lymph node LDC:MDC ratios induced by the protective immunogen were significantly lower than the ratios induced by either immunization in which the nonprotective immunogen was present. In contrast, mice given the nonprotective immunogen had LDC:MDC ratios similar to those of naive mice. Our data indicate that lymph node Langerhans cells and MDC are APC needed for induction of the protective response. The predominance of LDC in mice undergoing nonprotective responses suggests that lymph node LDC, like splenic LDC, are negative regulators of CMI responses. In addition to showing DC subsets associated with functional differences, our data suggest that the LDC:MDC balance, which can be modulated by the Ag, determines whether protective or nonprotective anticryptococcal CMI responses develop.

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Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments

et al. 2000

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Three populations of mouse lymph node dendritic cells with different origins and dynamics

Cited by 57 publications

References 0 publications

Langerhans Cells That Have Matured In Vivo in the Absence of T Cells Are Fully Capable of Inducing a Helper CD4 as Well as a Cytotoxic CD8 Response

Langerhans Cells That Have Matured In Vivo in the Absence of T Cells Are Fully Capable of Inducing a Helper CD4 as Well as a Cytotoxic CD8 Response

Dendritic Cells in the Induction of Protective and Nonprotective Anticryptococcal Cell-Mediated Immune Responses

Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments

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