Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments

Nolte, Martijn A.; Hoen, Esther N. M. Nolte‐‘t; Stijn, Amber van; Kraal, Georg; Mebius, Reina E.

doi:10.1002/1521-4141(200002)30:2<626::aid-immu626>3.0.co;2-h

Cited by 60 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The later raises a yet unresolved question of how Gr1 ϩ Mac-1 ϩ cells in the peripheral blood induce down-regulation. As mentioned above, T cells in the spleen of the P. gingivalistreated mice were localized to the red pulp zone, which is enriched with Gr1 ϩ cells (22). Moreover, we demonstrate that the percentage of Gr1 ϩ Mac-1 ϩ cells in the spleen of mice exhibiting chronic inflammation increases tremendously, similar to that described in tumor-bearing hosts (27)(28)(29)(30).…”

Section: Discussionsupporting

confidence: 82%

“…The red pulp is mainly populated by macrophages and is the zone in which erythrocytes are eliminated. The white pulp, on the other hand, contains mainly lymphocytes and dendritic cells, which are separated from the red pulp by the marginal sinus (22). We therefore examined the distribution of T cells in the spleens and lymph nodes using immunohistochemical analysis with anti-CD3 Abs (Fig.…”

Section: Mice Under Chronic Inflammatory Conditions Exhibit Disorganimentioning

confidence: 99%

See 1 more Smart Citation

TCR ζ Down-Regulation under Chronic Inflammation Is Mediated by Myeloid Suppressor Cells Differentially Distributed between Various Lymphatic Organs

Ezernitchi

Vaknin

Cohen-Daniel

et al. 2006

The Journal of Immunology

165

125

View full text Add to dashboard Cite

T cell AgR ζ chain down-regulation associated with T cell dysfunction has been described in cancer, infectious, and autoimmune diseases. We have previously shown that chronic inflammation is mandatory for the induction of an immunosuppressive environment leading to this phenomenon. To identify the key immunosuppressive components, we used an in vivo mouse model exhibiting chronic inflammation-induced immunosuppression. Herein, we demonstrate that: 1) under chronic inflammation secondary lymphatic organs display various immunological milieus; ζ chain down-regulation and T cell dysfunction are induced in the spleen, peripheral blood, and bone marrow, but not in lymph nodes, correlating with elevated levels of Gr1+Mac-1+ myeloid suppressor cells (MSC); 2) MSC are responsible for the induction of such an immunosuppression under both normal and inflammatory conditions; and 3) normal T cells administered into mice exhibiting an immunosuppressive environment down-regulate their ζ expression. Such an environment is anticipated to limit the success of immunotherapeutic strategies based on vaccination and T cell transfer, which are currently under investigation for immunotherapy of cancer.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Mice Under Chronic Inflammatory Conditions Exhibit Disorganimentioning

confidence: 99%

TCR ζ Down-Regulation under Chronic Inflammation Is Mediated by Myeloid Suppressor Cells Differentially Distributed between Various Lymphatic Organs

Ezernitchi

Vaknin

Cohen-Daniel

et al. 2006

The Journal of Immunology

165

125

View full text Add to dashboard Cite

show abstract

“…In particular, the splenic white pulp is structurally similar to a lymph node and contains discrete areas of T and B cells, whereas the red pulp comprises mainly macrophages and granulocytes [1]. We and others [2,3] have shown previously that most dendritic cells (DC) are distributed in the two splenic compartments in the steady state and migrate massively to the T cell area upon systemic administration of LPS.…”

Section: Introductionmentioning

confidence: 99%

“…Access of the white pulp has been shown to be restricted to lymphocytes and DC, and the distribution of neutrophils was almost exclusively confined to the red pulp [1]. We therefore examined whether neutrophils may undergo a dynamic process as described for DC and migrate from the red pulp to the white pulp, where naïve T cells reside.…”

Section: Introductionmentioning

confidence: 99%

Injection of lipopolysaccharide induces the migration of splenic neutrophils to the T cell area of the white pulp: role of CD14 and CXC chemokines

Kesteman

Vansanten

Pajak

et al. 2007

Journal of Leukocyte Biology

View full text Add to dashboard Cite

There is increasing evidence that neutrophils are involved in the regulation of adaptive immunity. We therefore tested whether these cells may colocalize with T lymphocytes in lymphoid organs. Our results demonstrate that administration of the microbial product LPS induces the migration of neutrophils in the spleen from the red pulp and the marginal zone to the area of the white pulp where T cells reside. This movement is CD14-dependent, whereas the recruitment of neutrophils in the peritoneal cavity is increased in the absence of CD14. Our data further suggest the involvement of the chemokine MIP-2 and keratinocyte-derived chemokine and their receptor CXCR2. We conclude that neutrophils may interact with naïve T cells upon infection/inflammation and that the migration of neutrophils in the lymphoid organs and in the periphery is regulated differently by a signal transduced by CD14 J. Leukoc. Biol. 83: 640 -647; 2008.

show abstract

“…It represents the largest single lymphoid organ and performs a critical immunological role reflected in the highly organized arrangement of lymphoid and accessory cells within the white pulp (for a review, see Fu and Chaplin, 1999). The lymphocytic composition of the white pulp is different from the red pulp, which also hosts a sizeable plasma cell population (Nolte et al, 2000;Garcia De Vinuesa et al, 1999). Whereas the white pulp is primarily engaged in mounting adaptive immune responses, the red pulp, composed of a stromal reticular network of endothelial sinusoids, macrophages, erythroid and accessory cells, performs a digestive function, clearing damaged and senescent red blood cells from the circulation.…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of ferroportin 1 interferes with spleen organogenesis in polycythaemia mice

et al. 2004

View full text Add to dashboard Cite

Regulatory interferences at the iron transporter ferroportin 1 (Fpn1) cause transient defects in iron homeostasis and erythropoiesis in polycythaemia(Pcm) mutant mice. The present study identified decreased Fpn1 expression in placental syncytiotrophoblast cells at late gestation as the mechanism of neonatal iron deficiency in Pcm mutants. Tissue specificity of embryonic Fpn1 dysregulation was evident from concomitant decreases in Fpn1 mRNA and protein expression in placenta and liver, as opposed to upregulation of Fpn1 protein despite decreased transcript levels in spleen, implicating post-transcriptional regulation of Fpn1. Dysregulation of Fpn1 and decreased iron levels in Pcm mutant spleens correlated with apoptotic cell death in the stroma, resulting in a semidominant spleen regression. At 7 weeks of age, a transient increase in spleen size in Pcm heterozygotes reflected a transient erythropoietin-mediated polycythemia. Structurally, Pcm mutant spleens displayed a severe defect in red pulp formation, including disruption of the sinusoidal endothelium, as well as discrete defects in white pulp organization during postnatal development. Reduced functional competence of the Pcmmutant spleen was manifested by an impaired response to chemically induced hemolytic anemia. Thus, aberrant Fpn1 regulation and iron homeostasis interferes with development of the spleen stroma during embryogenesis,resulting in a novel defect in spleen architecture postnatally.

show abstract

Isolation of the intact white pulp. Quantitative and qualitative analysis of the cellular composition of the splenic compartments

Cited by 60 publications

References 27 publications

TCR ζ Down-Regulation under Chronic Inflammation Is Mediated by Myeloid Suppressor Cells Differentially Distributed between Various Lymphatic Organs

TCR ζ Down-Regulation under Chronic Inflammation Is Mediated by Myeloid Suppressor Cells Differentially Distributed between Various Lymphatic Organs

Injection of lipopolysaccharide induces the migration of splenic neutrophils to the T cell area of the white pulp: role of CD14 and CXC chemokines

Dysregulation of ferroportin 1 interferes with spleen organogenesis in polycythaemia mice

Contact Info

Product

Resources

About