Three novel species of the Bacillus cereus group isolated from clinical samples in Japan

Abstract

Three Gram-positive bacterial strains, BML-BC004, BML-BC017 and BML-BC059, isolated from blood samples from three inpatients in Japan, were identified as members of Bacillus cereus using matrix-assisted laser desorption ionization time-of-flight MS. The 16S rRNA gene sequences of these three strains were more than 97.1 % similar to 18 type strains belonging to the B. cereus group. Whole-genome comparisons, using average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH), confirmed that the three strains represented three individual distinct species belonging to the B. cereus group. A phylogenetic tree showed that BML-BC004, BML-BC017 and BML-BC059 were located close to B. luti , B. mobilis and B. paramycoides , respectively. Based on these phylogenetic and phenotypic data, including values below the threshold for ANI and dDDH, the three strains should be classified as representing three different novel species of the B. cereus group: Bacillus sanguinis sp. nov., with type strain BML-BC004T (=DSM 111102T=JCM 34122T), Bacillus paramobilis sp. nov., with type strain BML-BC017T (=DSM 111100T=JCM 34124T) and Bacillus hominis sp. nov., with type strain BML-BC059T (=DSM 111101T=JCM 34125T).

Four Novel Leaderless Bacteriocins, Bacin A1, A2, A3, and A4 Exhibit Potent Antimicrobial and Antibiofilm Activities against Methicillin-Resistant Staphylococcus aureus

“…The Bacillus cereus group is comprised of a growing number of closely related species, including B. cereus , B. mycoides , B. pseudomycoides , B. thuringiensis , B. toyonensis , and B. weihenstephanensis ( 18 , 19 ). Studies have shown that the sequenced genomes of the B. cereus group strains contain a diverse array of bacteriocins, and a variety of different kinds of bacteriocins have been identified and characterized ( 20 – 22 ).…”

Bacillus rhizoplanae sp. nov. from maize roots

“…Later, Gupta et al [20] transferred three of the species which formed a stable outlying cluster in the Cereus clade, Bacillus acidiceler , Bacillus luciferensis and Bacillus solisilvae , to the novel genus Gottfriedia . Thereafter, three novel species with validated names were assigned to the core Cereus clade, Bacillus sanguinis , Bacillus paramobilis and Bacillus hominis [22]. All three species were isolates obtained from clinical sources [22].…”

“…Thereafter, three novel species with validated names were assigned to the core Cereus clade, Bacillus sanguinis , Bacillus paramobilis and Bacillus hominis [22]. All three species were isolates obtained from clinical sources [22]. One further species ‘Bacillus clarus ’ was described based on the characterization of a soil derived antibiotic-producing strain [23].…”