Four Novel Leaderless Bacteriocins, Bacin A1, A2, A3, and A4 Exhibit Potent Antimicrobial and Antibiofilm Activities against Methicillin-Resistant Staphylococcus aureus

Liu, Shu; Deng, Shulin; Liu, Hualin; Tang, Liang; Wang, Mengqi; Xin, Bingyue; Feng, Li

doi:10.1128/spectrum.00945-22

Cited by 9 publications

(4 citation statements)

References 52 publications

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“…A lack of activity against P. aeruginosa and S. enterica was expected given Gram-negative species are typically resistant to bacteriocins due to their reduced cell wall permeability [3]. Inactivity against these two organisms is consistent with leaderless bacteriocins produced by other Bacillus species that have been shown to be bactericidal via inducing bacterial membrane damage [32][33][34]. Given their comparable antimicrobial spectra, bawcin may exhibit antimicrobial activity through a similar mechanism.…”

Section: Antimicrobial Activity Of Bawcinmentioning

confidence: 87%

Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii

Budhwani,

Buragina,

Lang

et al. 2023

IJMS

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The rise of drug-resistant bacteria is a major threat to public health, highlighting the urgent need for new antimicrobial compounds and treatments. Bacteriocins, which are ribosomally synthesized antimicrobial peptides produced by bacteria, hold promise as alternatives to conventional antibiotics. In this study, we identified and characterized a novel leaderless bacteriocin, bawcin, the first bacteriocin to be characterized from a Bacillus wiedmannii species. Chemically synthesized and purified bawcin was shown to be active against a broad range of Gram-positive bacteria, including foodborne pathogens Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes. Stability screening revealed that bawcin is stable over a wide range of pH (2.0–10.0), temperature conditions (25–100 °C), and against the proteases, papain and pepsin. Lastly, three-dimensional structure homology modeling suggests that bawcin contains a saposin-fold with amphipathic helices and a highly cationic surface that may be critical for membrane interaction and the subsequent cell death of its targets. This study provides the foundational understanding of the activity and properties of bawcin, offering valuable insights into its applications across different antimicrobial uses, including as a natural preservative in food and livestock industries.

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Section: Antimicrobial Activity Of Bawcinmentioning

confidence: 87%

Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii

Budhwani,

Buragina,

Lang

et al. 2023

IJMS

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“…The bacterial strain Bacillus sp. TL12, isolated from soil by Liu et al, produces the antibiofilm‐active bacteriocin bacin A2 69 . Additionally, bacteriocins from soil sources are also produced by non‐ Bacillus species, such as Stenocins produced by Stenotrophomonas , ST110LD produced by Leuconostoc citreum ST110LD and BAC‐IB17 produced by S. aureus 70–72 …”

Section: Sources Of Natural Bacteriocin Isolates and Preparation Meth...mentioning

confidence: 99%

Impact of bacteriocins on multidrug‐resistant bacteria and their application in aquaculture disease prevention and control

Chen,

Liu,

Liu

et al. 2024

Reviews in Aquaculture

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Aquaculture plays an important role in meeting human demand for animal protein. Disease is a critical factor that restricts the green and healthy development of aquaculture. Intensive aquaculture practices can increase the susceptibility of aquatic animals to pathogens due to environmental stress. Antibiotics are commonly used for disease prevention and control, but their frequent use in aquaculture can increase the risk of antibiotic‐resistant bacteria and resistance gene transmission, posing a threat to the health of aquatic animals and humans. Recent studies have shown that bacteriocins have inhibitory effects on different species of bacteria, fungi and viruses, and can even affect natural resistance structures such as bacterial biofilms, presenting promising prospects as antimicrobial agents. Bacteriocins have potential applications in various fields such as agriculture, food and medicine, but limited research has been conducted on their impact on aquaculture disease prevention and control, and the underlying mechanisms remain to be explored. Therefore, this review aims to summarise the classification, sources, preparation methods, antibacterial activity against multidrug‐resistant bacteria and application of bacteriocins as antimicrobial agents in aquaculture disease prevention and control. In addition, limitations of bacteriocin application in aquaculture and future research directions are also discussed.

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“…To illustrate, Bacin A2, identified in Bacillus sphaericus TL12, could compromise the membrane integrity of S. aureus and methicillin-resistant S. aureus biofilms, resulting in disassembling of the biofilm. [32] Similarly, it was found that the novel bacteriocin AMYX6 and XJS01 could impair the membrane integrity and consequently inhibit biofilm formation in Salmonella Enteritidis and S. aureus respectively. [33,34] The antibiofilm mechanism of action of AMPs, however, is possibly ambiguous.…”

Section: Destruction Of Mature Biofilmsmentioning

confidence: 99%

Functional Materials to Overcome Bacterial Barriers and Models to Advance Their Development

Bali

Kamal

Mulla

et al. 2023

Adv Funct Materials

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With the emerging problem of antimicrobial resistance, the world is facing a slow but dangerous pandemic. While the discovery of novel antibiotics is reaching a nearly exhaustive end, new concepts for anti‐infective drugs are emerging. So‐called pathoblockers aim to de‐weaponize bacteria rather than just killing them. As the target of these molecules is typically located intracellularly, however, hitherto almost unnoticed biological barriers are emerging such as the biofilm matrix, the bacterial cell envelope, efflux pumps, and eventual bacterial metabolism. This leads to a new paradigm that is to maximize bacterial bioavailability. To overcome the bacterial barriers, especially when further optimization of the active molecules is not possible, functional materials are needed to engineer innovative delivery systems. Those may not only enable novel anti‐infective molecules to reach their targets, but will also improve the bacterial bioavailability of existing anti‐infectives. Additionally, there is a need for better infection models that allow studying drug effects on both the bacteria and the host in a relevant manner as needed for rational anti‐infective drug development.

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Four Novel Leaderless Bacteriocins, Bacin A1, A2, A3, and A4 Exhibit Potent Antimicrobial and Antibiofilm Activities against Methicillin-Resistant Staphylococcus aureus

Cited by 9 publications

References 52 publications

Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii

Characterization of the Novel Leaderless Bacteriocin, Bawcin, from Bacillus wiedmannii

Impact of bacteriocins on multidrug‐resistant bacteria and their application in aquaculture disease prevention and control

Functional Materials to Overcome Bacterial Barriers and Models to Advance Their Development

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