Three novel MC4R SNPs associated with growth traits in Hu sheep and East Friesian×Hu crossbred sheep

Wang, Yalei; Zhang, Jun; Meng, Chunhua; Zhang, Xiaojian; Wang, Ziyu; Fang, Yongfei; Mao, Dagan; Cao, Shulin

doi:10.1016/j.smallrumres.2015.02.007

Cited by 11 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This role has been shown to be evolutionarily conserved in vertebrates (Andersone et al, 2016). Allele and genotype distribution of MC4R gene have been described by many researchers in monogastric and polygastric animal such as in chicken (Yan et al, 2013), pig (Piorkoswka et al, 2010Davoli et al, 2012;Zhang et al, 2017), sheep (Song et al, 2014;Wang et al, 2015), cattle (Huang et al, 2010;Liu et al, 2010;Maharani et al, 2018) and buffalo (Deng et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

Latifah

Maharani

Kustantinah

et al. 2018

J. Indonesian Trop. Anim. Agric.

View full text Add to dashboard Cite

Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.

show abstract

Section: Introductionmentioning

confidence: 99%

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

Latifah

Maharani

Kustantinah

et al. 2018

J. Indonesian Trop. Anim. Agric.

View full text Add to dashboard Cite

show abstract

“…Based on SNP analysis of the nucleotide, it was found that g.306G/A and g.706C/A were identified in the coding region (Wang et al, 2015). In other species, 1426G/A MC4R gene mutation were found in pig (Piorkowska et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…According to Williams (2003), the restriction enzyme is a fundamental tool of molecular biology for diversity and in vivo function. Previous research used restriction enzyme for genotyping mammals was based on MC4R gene (Liu et al, 2010;Wang et al, 2015). Wang et al (2015) reported that restriction enzyme MspI, Kpn2I and NdeI were used for genotyping mammals.…”

Section: Discussionmentioning

confidence: 99%

“…Previous research used restriction enzyme for genotyping mammals was based on MC4R gene (Liu et al, 2010;Wang et al, 2015). Wang et al (2015) reported that restriction enzyme MspI, Kpn2I and NdeI were used for genotyping mammals. In other study, enzyme TaiI was used for PCR-RFLP and produced three genotypes in a Qinchuan cattle (Liu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…MC4R gene polymorphism have also been identified as genetic markers for ADG, FGR, back fat tickness, fatness, leanness, fat distribution, and feed intake in pig (Piorkowska et al, 2010;Galve et al, 2012;Fontanesi et al, 2013;Schroyen et al, 2015), life weight, ADG, carcass weight, back fat thickness, marbling, and dressing percentage in cattle (Zhang et al, 2009;Liu et al, 2010;Seong et al, 2011), body weight, body measurement, meat quality, back fat thickness, and loin-eye area in sheep (Zhang et al, 2009;Song et al, 2012;Zou et al, 2013;Wang et al, 2015) and body weight in Boer goat (Jun et al, 2013). However, no report on the MC4R gene study have been identified in Bligon goat.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat

Latifah¹,

Priyadi²,

Maharani³

et al. 2017

Med Pet

View full text Add to dashboard Cite

Characterization of the melanocortin-4 receptor gene from Spinibarbus hollandi and the association between its polymorphisms and S. hollandi growth traits

Yang

Shu

et al. 2017

Fish Sci

View full text Add to dashboard Cite

Three novel MC4R SNPs associated with growth traits in Hu sheep and East Friesian×Hu crossbred sheep

Cited by 11 publications

References 36 publications

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat

Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat

Characterization of the melanocortin-4 receptor gene from Spinibarbus hollandi and the association between its polymorphisms and S. hollandi growth traits

Contact Info

Product

Resources

About