Characterization of the melanocortin-4 receptor gene from Spinibarbus hollandi and the association between its polymorphisms and S. hollandi growth traits

Yang, Yang; Li, Qiang; Shu, Hu; Zhou, Huiqiang; X, Li; Hou, Liping

doi:10.1007/s12562-017-1125-x

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…In our previous work, we attempted to enhance the growth rate of S. hollandi by improving the rearing conditions and crossbreeding. The molecular markers associated with growth traits were developed in S. hollandi [17, 18]. However, studies on the effects of fasting and refeeding on the growing status of this species are deficient.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of skeletal muscle reveals starvation response and compensatory growth in Spinibarbus hollandi

et al. 2019

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BackgroundSpinibarbus hollandi is an economically important fish species in southern China. This fish is known to have nutritional and medicinal properties; however, its farming is limited by its slow growth rate. In the present study, we observed that a compensatory growth phenomenon could be induced by adequate refeeding following 7 days of fasting in S. hollandi. To understand the starvation response and compensatory growth mechanisms in this fish, the muscle transcriptomes of S. hollandi under control, fasting, and refeeding conditions were profiled using next-generation sequencing (NGS) techniques.ResultsMore than 4.45 × 108 quality-filtered 150-base-pair Illumina reads were obtained from all nine muscle samples. De novo assemblies yielded a total of 156,735 unigenes, among which 142,918 (91.18%) could be annotated in at least one available database. After 7 days of fasting, 2422 differentially expressed genes were detected, including 1510 up-regulated genes and 912 down-regulated genes. Genes involved in fat, protein, and carbohydrate metabolism were significantly up-regulated, and genes associated with the cell cycle, DNA replication, and immune and cellular structures were inhibited during fasting. After refeeding, 84 up-regulated genes and 16 down-regulated genes were identified. Many genes encoding the components of myofibers were significantly up-regulated. Histological analysis of muscle verified the important role of muscle hypertrophy in compensatory growth.ConclusionIn the present work, we reported the transcriptome profiles of S. hollandi muscle under different conditions. During fasting, the genes involved in the mobilization of stored energy were up-regulated, while the genes associated with growth were down-regulated. After refeeding, muscle hypertrophy contributed to the recovery of growth. The results of this study may help to elucidate the mechanisms underlying the starvation response and compensatory growth.

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Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of skeletal muscle reveals starvation response and compensatory growth in Spinibarbus hollandi

et al. 2019

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“…We have developed molecular markers associated with growth traits in S . hollandi [15] and recently observed the compensatory growth process in this species. Currently, studies on the effects of fasting on the growth rate and the compensatory growth of S .…”

Section: Introductionmentioning

confidence: 83%

“…In down-regulated DEGs, proopiomelanocortin (POMC) is an important precursor polypeptide, which can be enzymatically cleaved into many peptides, including melanotropins (α-MSH, β-MSH, and γ-MSH), adrenocorticotropin (ACTH), lipotropins, and endorphins [48]. These ligands closely associated with energy balance, glucose homeostasis and feeding behavior by negative regulation of energy metabolism [15,49]. The down-regulation of POMC is beneficial to diet intake.…”

Section: Discussionmentioning

confidence: 99%

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Effect of fasting and subsequent refeeding on the transcriptional profiles of brain in juvenile Spinibarbus hollandi

et al. 2019

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Starvation is a common stress in fish. The underlying molecular mechanisms associated with growth depression caused by feeding restriction and compensatory growth are not well understood. We investigated the effect of fasting and refeeding on the transcriptome profiles of brain in juvenile S . hollandi using RNA-seq. A total of 4.73 × 10 8 raw reads were obtained from nine brain samples. De novo transcriptome assembly identified 387,085 unigenes with 2.1×10 9 nucleotides. A total of 936 annotated unigenes showed significantly differential expression among the control, fasting, and fasting-refeeding groups. The down-regulated differentially expressed genes (DEGs) during fasting were mainly associated with cell cycle, DNA replication, and mitosis. The up-regulated DEGs were mainly related to glucose and lipid metabolism, material transportation, and transcription factors. Most decreased DEGs during fasting were restored to normal levels after refeeding. Comparing with the control group, genes associated with protein synthesis, stimulus response, and carbohydrate metabolism were significantly over-expressed and pro-opio melanocortin (POMC) was down-regulated during the refeeding period. In conclusion, fish mobilized stored energetic materials and reduced energy consumption to prolong survival during fasting. After refeeding, the down-regulation of DEGs, e.g., POMC may be associated with compensatory growth. Up-regulation of DEGs related to ribosomal protein, stimulus response, and carbohydrate metabolism may contribute to eliminate negative effect of starvation on brain. This study provided the first transcriptome data related with impact of short-time starvation and refeeding in S . hollandi brains.

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Identification of Candidate Growth-Related SNPs and Genes Using GWAS in Brown-Marbled Grouper (Epinephelus fuscoguttatus)

Yang

et al. 2020

Mar Biotechnol

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Characterization of the melanocortin-4 receptor gene from Spinibarbus hollandi and the association between its polymorphisms and S. hollandi growth traits

Cited by 7 publications

References 26 publications

Transcriptional profiling of skeletal muscle reveals starvation response and compensatory growth in Spinibarbus hollandi

Transcriptional profiling of skeletal muscle reveals starvation response and compensatory growth in Spinibarbus hollandi

Effect of fasting and subsequent refeeding on the transcriptional profiles of brain in juvenile Spinibarbus hollandi

Identification of Candidate Growth-Related SNPs and Genes Using GWAS in Brown-Marbled Grouper (Epinephelus fuscoguttatus)

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