Three new species of gall-forming psyllids (Hemiptera: Psylloidea) from Papua New Guinea, with new records and notes on related species

Abstract

Three new species of gall-forming psyllids (Hemiptera: Psylloidea) in the families Triozidae and Phacopteronidae are described from Papua New Guinea: Trioza incrustata Percy, sp. nov. makes enclosed leaf margin galls on Celtis philippensis (Cannabaceae), Trioza grallata Percy, sp. nov. makes enclosed leaf surface galls on Elaeocarpus schlechterianus (Elaeocarpaceae), and Cornegenapsylla allophyli Malenovský and Percy, sp. nov. makes enclosed leaf margin galls on Allophylus cobbe (Sapindaceae). Descriptions of the adult and immature morphology for these new species are provided, and differences in adult and immature morphology between Cornegenapsylla allophyli and Cornegenapsylla sinica Yang and Li, 1982 (the type species of Cornegenapsylla) are illustrated. We report new records for Papua New Guinea of Pseudophacopteron tuberculatum (Crawford, 1912) on Alstonia sp. (Apocynaceae), Pauropsylla triozoptera Crawford, 1913 on Ficus trachypison (Moraceae) and Pauropsylla udei Rübsaamen, 1899 on Ficus variegata, with descriptions of the variation found in the latter two widespread Asian species. Pauropsylla reticulata Mathur, 1975 is synonymized with P. udei. Mitochondrial DNA barcodes for the new species, as well as for P. triozoptera and P. udei are provided.

Salix transect of Europe: structured genetic variation and isolation-by-distance in the nettle psyllid, Trioza urticae (Psylloidea, Hemiptera), from Greece to Arctic Norway

“…Two regions of mtDNA; cytochrome c oxidase subunit I (COI) and cytochrome B (cytB) were amplified using standard PCR procedures as described previously [Percy 2003, Percy et al 2015]. The amplified PCR products were then sequenced using the Sanger sequencing method [Sanger et al 1977].…”

“…DNAs from 96 specimens, including 87 samples from the eastern European megatransect and 9 samples from western Europe, were extracted using a Qiagen DNeasy Blood and Tissue Kit [QIAGEN Ltd., Manchester, UK]. Two regions of mtDNA; cytochrome c oxidase subunit I (COI) and cytochrome B (cytB) were amplified using standard PCR procedures as described previously [ Percy 2003 , Percy et al 2015 ]. The amplified PCR products were then sequenced using the Sanger sequencing method [ Sanger et al 1977 ].…”

Molecular and morphometric identification of pistachio psyllids with niche modeling ofAgonoscena pistaciae(Hemiptera: Aphalaridae)

“…Two mitochondrial gene regions, mtCO1 and cytb, were amplified as DNA barcodes (Percy et al, 2016). The polymerase chain reaction for mtCO1 and cytb genes follows those described in Percy et al (2016) under the following conditions: denaturation step of 3 min at 94°C, followed by 40 cycles of denaturation for 30 s at 92°C, annealing for 40 s at 50°C (mtCO1 gene) or 56°C (for cytb gene), and extension for 1 min at 72°C, followed by a final extension step of 10 min at 72°C. The primers were obtained to amplify mtCO1 and cytb genes in Simon et al (1994) and Percy et al (2016), respectively (table 2).…”

“…Usually, the 5′-end of the mitochondrial cytochrome c oxidase subunit I gene, mtCO1 , is used as a standard in DNA barcoding (Hebert et al ., 2003 b ), though it does not work properly in some taxa (Park et al ., 2011). The mtDNA has been widely used for barcoding psyllids (Percy et al ., 2016; Taylor et al ., 2016; Percy, 2017; Martoni et al ., 2018). As the barcoding areas of mtDNA, mtCO1, and cytochrome b ( cytb ) are short (typically 472 bp for mtCO1 and 385 bp for cytb ), they are easy to amplify and sequence successfully even when the DNA is fairly degraded.…”

Making the most of your host: the Metrosideros-feeding psyllids (Hemiptera, Psylloidea) of the Hawaiian Islands

“… Crawford (1918) thought that the galling habit in itself indicated an affiliation between Hawaiian and Asian species as there is an unusually high percentage of gallers in some Asian psyllid faunas: in Taiwan and Japan 50% or more of the described psyllid species are gall making, and notably these oriental galling faunas are dominated by taxa in the family Triozidae ( Yukawa and Masuda 1996 ; Yang and Raman 2007 ; Yang et al 2013 ; Percy et al 2015 ). What is particularly remarkable in Pariaconus is that within this lineage and over a relatively short period of evolutionary time, there have evolved many different galling habits as well as completely free-living species, and these shifts have all occurred within an insular radiation over a few million years.…”