Molecular and morphometric identification of pistachio psyllids with niche modeling of<i>Agonoscena pistaciae</i>(Hemiptera: Aphalaridae)

Lashkari, Mohammadreza; Burckhardt, Daniel; Gushki, Roghayeh Shamsi

doi:10.1017/s0007485319000555

Cited by 4 publications

(8 citation statements)

References 52 publications

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“…However, the identification of P. fraxini (based on samples collected in 1902) needs to be confirmed (Burckhardt and Lauterer 1993). Also, P. machinosus has been reported from Kerman province in 2016 (Lashkari et al in press). …”

Section: Introductionmentioning

confidence: 99%

Identification, sexual dimorphism, and allometric effects of three psyllid species of the genus Psyllopsis by geometric morphometric analysis (Hemiptera, Liviidae)

Gushki¹,

Lashkari²,

Mirzaei³

2018

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Jumping plant lice (Hemiptera: Psylloidea) are considered important vectors of plant diseases and also economically important pests in agriculture and forest ecosystems. Three psyllid species Psyllopsis repens Loginova, 1963, Psyllopsis securicola Loginova, 1963, and Psyllopsis machinosus Loginova, 1963 associated with the ash tree Fraxinus are morphologically very similar. So far, their distinction has been possible only by comparing their male and female genitalia. In this research, forewing shape and size characteristics, sexual dimorphism and their allometric effects, using geometric morphometric analysis, were examined for identification purposes. The results showed significant differences in wing shape and size between the species studied. Based on the results, two species P. machinosus and P. securicola can be differentiated with the vein M1+2, as in P. securicola the vein M1+2 is located between Rs and M3+4 veins, but the vein M1+2 is closer to the vein M3+4 in P. machinosus; also, P. repens can be differentiated from the two species P. machinosus and P. securicola by vein M. Hence, the veins M1+2, M3+4, Rs and M were the most important wing characters for discrimination of the three species, especially in the field. The analysis also showed significant differences in wing shape and size between male and female of the three species, and the allometric analysis showed that significant shape differences still remain in constant size in P. machinosus and P. repens. Geometric changes in the forewings of both sexes for the three species are illustrated.

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Section: Introductionmentioning

confidence: 99%

Identification, sexual dimorphism, and allometric effects of three psyllid species of the genus Psyllopsis by geometric morphometric analysis (Hemiptera, Liviidae)

Gushki¹,

Lashkari²,

Mirzaei³

2018

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“…Although geometric morphometrics has been used to separate species in different taxa, including Hemiptera (Shamsi Gushki et al ., 2018; Lashkari et al ., 2020), it has not been applied to Diaphorina , a psyllid genus with morphologically similar species that are notoriously difficult to identify. The forewing shape of many Diaphorina species is oblong oval, widening toward the apical quarter as in the three studied species.…”

Section: Discussionmentioning

confidence: 99%

“…In many insect groups, the COI gene serves as a good standard for barcodes (Hebert et al ., 2004a, 2004b; Hajibabaei et al ., 2006; Ashfaq et al ., 2014a, 2014b, 2015). The primers used in the current study showed their effectiveness for the studied species and were also successfully applied in other studies on Psylloidea (Percy et al ., 2016; Lashkari et al ., 2019, 2020). Although the climatic conditions vary between the collection sites, no intraspecific genetic difference was found between populations and individuals of each studied species, including all Iranian geographic populations of D. citri .…”

Section: Discussionmentioning

confidence: 99%

“…The 5′ half of the cytochrome oxidase 1 ( COI ) gene has been used as the barcode locus for most animals in DNA barcoding (Hebert et al ., 2003a). The approach has been successfully applied to several groups of psyllids (Percy et al ., 2016; Percy, 2017; Taylor et al ., 2016; Martoni et al ., 2018; Lashkari et al ., 2019; Cho et al ., 2020; Lashkari et al ., 2020). The barcode locus used for psyllids is short (typically 472 bp), easy to amplify and it can be successfully sequenced even when the DNA is fairly degraded and fragmented.…”

Section: Introductionmentioning

confidence: 99%

“…Morphometrics is a useful method for analyzing shape and addressing a wide range of questions including evaluating phenetic differences between taxa (e.g., Burckhardt and Basset, 2000; Serbina et al ., 2015; Serbina and Mennecart, 2018; Shamsi Gushki et al ., 2018; Lashkari et al ., 2020). In geometric morphometrics, the shape is usually conceptualized and has been defined as those components of form which remain after removing the size (Kendall, 1977; MacLeod, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Molecular, morphometric and digital automated identification of three Diaphorina species (Hemiptera: Liviidae)

Lashkari

Burckhardt

Kashef

2021

Bull. Entomol. Res.

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Diaphorina is a species-rich genus, native to the tropics and subtropics of the Old World, particularly of more arid regions. One of the species, Diaphorina citri, is the economically most important pest of citrus. Diaphorina species are morphologically similar which makes their identification difficult. In this study, the accuracy of DNA barcoding, using mitochondrial cytochrome c oxidase subunit 1 (COI), geometric morphometrics of the forewing and digital image processing methods were tested for identification of the three Diaphorina species: D. chobauti, D. citri and D. zygophylli. Moreover, the published COI sequences of D. citri, D. communis and D. lycii obtained from Genbank were used for cluster analyses. DNA barcodes for D. chobauti and D. zygophylli are deposited in Genbank for the first time. The results of the molecular and geometric morphometric analyses are congruent and place D. chobauti as the sister taxon of the other Diaphorina species. The geometric morphometric analysis shows that in D. zygophylli the fore margin is slightly curved proximally and sharply bent distally, while in D. chobauti and D. citri it is straight proximally and weakly bent distally. The results of digital image processing show that the distribution of the dark pattern differs consistently in the three studied species.

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Salicylic Acid Induced Resistance in Drought-Stressed Pistachio Seedlings Influences Physiological Performance of Agonoscena pistaciae (Hemiptera: Aphalaridae)

Haghighi

Hosseininaveh

Talebi

et al. 2021

Journal of Economic Entomology

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Induced host plant resistance is a potential approach to insect and disease management. Salicylic acid (SA) acts as a signal molecule to induce resistance in plants against sap-sucking insects. The effects of salicylic acid-induced resistance against common pistachio psylla, Agonoscena pistaciae Burckhardt and Lauterer, were investigated in well-watered and drought-stressed pistachio, Pistacia vera L. cv. Akbari, seedlings. Agonoscena pistaciae exhibited a significant preference for plants treated with SA as compared with untreated controls or those subjected to drought stress. Plants subjected to both drought stress and SA treatment were equivalently colonized as compared with control plants but were more attractive than those subjected to drought stress alone. Psyllid mortality increased on plants subjected to simultaneous drought stress and SA treatment as compared with controls. Salicylic acid treatment mediated production of defensive enzymes in plants, including superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol peroxidase (GPX) and polyphenol oxidase (PPO), as well as that of other metabolites such as phenol, malondialdehyde (MDA), H2O2, free amino acids, and pigments via phenylpropanoid pathways under conditions of drought. Despite increases in activity of detoxification (glutathione S transferase, carboxylesterase) and antioxidative (SOD, CAT, APX, phenoloxidase, GPX) enzymes in psyllids, reduced survival of A. pistaciae on drought stressed and SA-treated plants was likely caused by excessive H2O2 and high phenolic content in treated plants. Based on our results, we postulate that salicylic acid-induced defense against A. pistaciae under drought conditions could be manipulated to enhance antibiosis against this key pest in pistachio orchards.

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Molecular and morphometric identification of pistachio psyllids with niche modeling ofAgonoscena pistaciae(Hemiptera: Aphalaridae)

Cited by 4 publications

References 52 publications

Identification, sexual dimorphism, and allometric effects of three psyllid species of the genus Psyllopsis by geometric morphometric analysis (Hemiptera, Liviidae)

Identification, sexual dimorphism, and allometric effects of three psyllid species of the genus Psyllopsis by geometric morphometric analysis (Hemiptera, Liviidae)

Molecular, morphometric and digital automated identification of three Diaphorina species (Hemiptera: Liviidae)

Salicylic Acid Induced Resistance in Drought-Stressed Pistachio Seedlings Influences Physiological Performance of Agonoscena pistaciae (Hemiptera: Aphalaridae)

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