Three New Methylobacterium Species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov.

Green, P. N.; Bousfield, I. J.; Hood, Derek W.

doi:10.1099/00207713-38-1-124

Cited by 90 publications

(43 citation statements)

References 7 publications

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“…Anaerobic thiosulphate oxidation was tested with nitrate [8]. Sulphate reducers (SRB) were quantified on lactate [9], methylotrophic bacteria were quantified with methanol [10], and chemoorganotrophic microorganisms (COT) were quantified on DEV gelatine medium (MERCK, Germany, No 10685) with 10% of the original organic substrate content. Sulphur compounds were quantified by HPLC/IC techniques [11].…”

Section: Methodsmentioning

confidence: 99%

Preliminary Evidence for a Sulphur Cycle in Movile Cave, Romania

Rohwerder

Sand

Lascu

2003

Acta Biotechnologica

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SummaryIn order to investigate the food chain and energy balance of the chemolithoautotrophically-based ecosystem of the sulphur spring in Movile Cave as a model system for extraterrestrial life, a first sampling campaign was done. Microbial diversity and activity were analysed by MPN-enumeration methods and microcalorimetry, respectively. In addition, a speciation of the inorganic sulphur compounds by HPLC and IC techniques was performed. Metabolic activities were predominantly connected with thick microbial mats floating on the water surface of the cave. These mats showed an aerobic heat evolution of about 1200 µW/g and contained about 500 µmol/g elemental sulphur. In contrast, other samples collected from cave water, sediment and rock exhibited only activities of maximal 40 to 60 µW/g and contained only up to 2.5 µmol/g elemental sulphur. As the main primary producers aerobic and facultatively anaerobic sulphur oxidisers were identified at high numbers, occasionally exceeding 10 7 CFU/g. Methylotrophic bacteria were present in all samples at up to 10 6 CFU/g, indicating the important role of C1 metabolism for the cave ecosystem. Although reduced sulphur species were biologically oxidised to sulphuric acid, the pH values of the samples ranged from 6.5 to 8.2 due to the high buffering capacity of the cave walls, which consisted mainly of limestone. Surprisingly, not only neutrophilic but also extremely acidophilic bacteria were detected. Sulphate reducers were present in both aerobic and anaerobic zones. The data presented suggest a close interdependence of sulphur oxidation and reduction as well as carbon dioxide and other C1 compound metabolism in the most biologically active zone of the cave ecosystem, i.e. the floating microbial mats.

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Section: Methodsmentioning

confidence: 99%

Preliminary Evidence for a Sulphur Cycle in Movile Cave, Romania

Rohwerder

Sand

Lascu

2003

Acta Biotechnologica

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“…Methylobacterium extorquens ( N C I B 9133), previously known as Pseudomonas AM1 or Methylobacterium AM1 (Green et al, 1988), was grown at 30 "C on the medium of MacLennan et al (1971) as described by O' Keeffe & Anthony (1980b), with methanol (0.5%) as carbon source unless otherwise stated. The moxD mutant (UV9), described by Nunn & Lidstrom (1988a, b), was grown on the same medium as the wild-type strain except that the carbon source was 0.5% methylamine.…”

Section: Methodsmentioning

confidence: 99%

“…It was recently classified and renamed, and shown to be similar to other pink facultative methylotrophs such as M. organophilum and M . zatmanii (Green & Bousefield, 1982;Green et al, 1988). It oxidizes methanol by way of the periplasmic quinoprotein methanol dehydrogenase (MDH) which interacts directly with a specific cytochrome c (called cytochrome cL).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a novel soluble c-type cytochrome in a moxD mutant of Methylobacterium extorquens AM1

Day

Nunn²,

Anthony³

1990

Journal of General Microbiology

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Methylobacterium extorquens AM1 contains a novel c-type cytochrome, called cytochrome c-553, previously thought to be a precursor of the electron acceptor (cytochrome cL) for methanol dehydrogenase. Its amino acid composition and serological characteristics show that it has no structural relationship to cytochrome cL. It usually comprises less than 5% of the total c-type cytochromes. In a moxD mutant, which contains neither methanol dehydrogenase nor cytochrome cL, it comprises 30% of the soluble cytochrome and it has been purified and characterized from that mutant. Cytochrome c-553 is large ( M , 23000), acidic and monohaem, with a redox potential of 194 mV. It reacts rapidly and completely with CO but is not autoxidizable. It is not autoreducible, and it is not an electron acceptor from methanol dehydrogenase or methylamhe dehydrogenase, nor an important electron donor to the oxidase. It is able to accept electrons from cytochrome cL and to donate electrons to cytochrome cH. It is present in the soluble fraction (presumably periplasmic) and membrane fraction of wild-type bacteria during growth on a wide range of growth substrates, but its function in these bacteria or in the moxD mutant has not been determined.

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“…It includes strictly aerobic, Gram-negative, rod-shaped, pink-pigmented, facultatively methylotrophic (PPFM) bacteria, which can grow on single carbon compounds such as formate, formaldehyde and methanol as the sole source of carbon and energy, as well as on a wide range of multi-carbon growth substrates (Green, 2001). At the time of writing, the genus comprises 20 recognized species (Patt et al, 1976;Bousfield & Green, 1985;Green et al, 1988;Urakami et al, 1993;Wood et al, 1998; Doronina et al, 2000Doronina et al, , 2002 McDonald et al, 2001;Van Aken et al, 2004;Jourand et al, 2004; Gallego et al, 2005a, b, c), with Methylobacterium organophilum as the type species (Patt et al, 1976).Members of the genus Methylobacterium are ubiquitous in terrestrial habitats (Green & Bousfield, 1981, including soil, dust, freshwater, tap water systems, lake sediments, leaf surfaces and nodules, rice grains and air, and as contaminants in various products and processes. The PPFM bacteria are strict aerobes and can be isolated from almost any freshwater environment where some dissolved oxygen exists, e.g.…”

mentioning

confidence: 99%

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

Gallego

Garcı́a

Ventosa

2006

International Journal of Systematic and Evolutionary Microbiology

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A pink-pigmented, facultatively methylotrophic strain, AR27 T , isolated from the drinking water distribution system of Seville, Spain, was characterized taxonomically. Cells were Gram-negative rods, motile, non-spore-forming and strictly aerobic. Growth in liquid media was flocculant whereas on solid media growth produced colonies that usually adhered to the agar surface. On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (63?6 mol%), strain AR27 T (=CECT 7069 T =CCM 7305 T ) is proposed as the type strain of a novel species of the genus Methylobacterium, Methylobacterium adhaesivum sp. nov.The genus Methylobacterium belongs to the a-2 subclass of the Proteobacteria and has the serine pathway for formaldehyde assimilation. It includes strictly aerobic, Gram-negative, rod-shaped, pink-pigmented, facultatively methylotrophic (PPFM) bacteria, which can grow on single carbon compounds such as formate, formaldehyde and methanol as the sole source of carbon and energy, as well as on a wide range of multi-carbon growth substrates (Green, 2001). At the time of writing, the genus comprises 20 recognized species (Patt et al., 1976;Bousfield & Green, 1985;Green et al., 1988;Urakami et al., 1993;Wood et al., 1998; Doronina et al., 2000Doronina et al., , 2002 McDonald et al., 2001;Van Aken et al., 2004;Jourand et al., 2004; Gallego et al., 2005a, b, c), with Methylobacterium organophilum as the type species (Patt et al., 1976).Members of the genus Methylobacterium are ubiquitous in terrestrial habitats (Green & Bousfield, 1981, including soil, dust, freshwater, tap water systems, lake sediments, leaf surfaces and nodules, rice grains and air, and as contaminants in various products and processes. The PPFM bacteria are strict aerobes and can be isolated from almost any freshwater environment where some dissolved oxygen exists, e.g. from tap water systems (Gräf & Bauer, 1973) and from stratified lake systems (Hanson, 1980). Furthermore, species of this genus have been isolated from hospital environments (Gilardi & Faur, 1984), where they may pose a threat as opportunistic pathogens to seriously ill patients.A sampling campaign in April 2004 to identify culturable and non-culturable bacteria present in the drinking water distribution system of Seville, Spain, led to isolation of a slowly growing pink-pigmented bacterium. This isolate was studied phenotypically, phylogenetically and genotypically. Based on these characteristics we suggest that strain AR27 T represents a novel species of the genus Methylobacterium.Drinking water samples (25 l) were concentrated by using a tangential flow filtration system (Filtron), plated on plate count agar (PCA; Difco) and R2A (Difco) and incubated at 28 uC for 7 days. Distinct colonies were picked in order to obtain pure cultures. Pink-pigmented colonies were selected and studied in more detail. One of these strains, AR27 T , showed a characteristic growth that differentiated it from the remaining pink-pigmented colonies isolated. Strain AR27 ...

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Three New Methylobacterium Species: M. rhodesianum sp. nov., M. zatmanii sp. nov., and M. fujisawaense sp. nov.

Cited by 90 publications

References 7 publications

Preliminary Evidence for a Sulphur Cycle in Movile Cave, Romania

Preliminary Evidence for a Sulphur Cycle in Movile Cave, Romania

Characterization of a novel soluble c-type cytochrome in a moxD mutant of Methylobacterium extorquens AM1

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

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