Three new Hermatomyces species (Lophiotremataceae) on Pandanus odorifer from Southern Thailand 

Abstract

Investigations on microfungi on Pandanus odorifer (Pandanaceae) from southern Thailand resulted in the discovery of three new species of Hermatomyces (Lophiotremataceae). Phylogenetic analyses of combined LSU, ITS, SSU, RPB2 and TEF1 sequence data showed that our new taxa cluster with Hermatomyces species and were well separated from Aquasubmersa and Lophiotrema species in Lophiotremataceae. We introduce the new species, Hermatomyces krabiensis, H. pandanicola and H. saikhuensis, with illustrated accounts. Evidence for demarcation of the three new species is provided using morphology and phylogenetic analyses. This is the first report of Hermatomyces on Pandanus species.

Revision of Lophiotremataceae (Pleosporales, Dothideomycetes): Aquasubmersaceae, Cryptocoryneaceae, and Hermatomycetaceae fam. nov.

“…As a sexual morph of this genus has not been reported and a phylogenetic study has not been performed, this genus has long been treated as ‘ incertae sedis’ within Ascomycota ( Wijayawardene et al 2012 ). Doilom et al (2016) and Tibpromma et al (2016) have suggested that this genus belongs to Lophiotremataceae on the basis of their phylogenetic analyses using SSU, LSU, tef1 , and rpb2 . Our analyses using additional species of Lophiotremataceae and its related taxa revealed that Hermatomyces is distantly related to Lophiotremataceae ( Fig.…”

“…Phylogenetic analyses using sequences of nuclear rDNA small subunit (18S; SSU), internal transcribed spacer (ITS) and large subunit (28S; LSU) regions and translation elongation factor 1-α ( tef1 ) and DNA-directed RNA polymer-ase II second largest subunit ( rpb2 ) genes have suggested that Aquasubmersa and Hermatomyces are closely related to Lophiotrema ( Doilom et al 2016 , Tibpromma et al 2016 ). On the basis of their phylogenetic studies, Doilom et al (2016) and Tibpromma et al (2016) proposed that Aquasubmersa and Hermatomyces are additional members of Lophiotremataceae . Another genus, Cryptocoryneum , is characterised by having stromatic sporodochia and cheiroid conidia ( Schoknecht & Crane 1977 ).…”

Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

“…The authors have been investigating saprobic and endophytic fungi associated with Pandanaceae (Tibpromma et al 2016a, b, c, 2017a, b) and, in this study, taxonomic details are presented regarding the endophytic fungi that were isolated. Pandanaceae are monocotyledonous plants.…”

Muyocopron garethjonesii sp. nov. (Muyocopronales, Dothideomycetes) on Pandanus sp.

“…China). Polymerase chain reactions (PCR) were used to amplify partial gene regions LSU (Vilgalys & Hester 1990) and SSU (White et al 1990) using primers and conditions as outlined in Tibpromma et al (2016b). The total volume of PCR mixtures for amplifications were 25 μL containing 8.5 μL ddH 2 O, 12.5 μL 2× Easy Taq PCR Super Mix (mixture of Easy Taq TM DNA Polymerase, dNTPs, and optimized buffer (Beijing Trans Gen Biotech Co., Chaoyang District, Beijing, PR China), 2 μL of DNA template, 1 μL of each forward and reverse primers (10 pM).…”