Three New Constituents From the Parasitic Plant Balanophora laxiflora

Abstract

Three new constituents (1-3) were isolated from the whole plant of Balanophora laxiflora. Their chemical structures were elucidated by extensive analysis of high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. Absolute configurations of new compounds were determined by circular dichroism spectra and modified Mosher's method. The isolated compounds weakly inhibited on both NO production and COX-2 mRNA expression in RAW264.7 macrophages.

Anti-inflammatory effect of a triterpenoid from Balanophora laxiflora: results of bioactivity-guided isolation

“…Fifteen compounds isolated from the ethyl acetate fraction and aqueous residue, plus three previously identified compounds (compounds 16 , 17 , and 18 with chemical structures as shown in Supplementary data Fig. S2) ( Tai et al., 2019 )) derived from the aqueous fraction of B. laxiflora , were next screened for inhibition of LPS-induced COX-2 expression in Raw 264.7 macrophages. Of these, only 1 and 5 suppressed COX-2 mRNA expression upon LPS stimulation, with the reduction induced by 1 being more significant ( Figure 3 A).…”

“…In addition, a recent study by our colleagues led to the isolation of a new B . laxiflora tannin that has modest anti-inflammatory effects in Raw 264.7 macrophages ( Tai et al., 2019 ). These findings suggest that B .…”

Three New Glycosides From the Stems of Derris elliptica

“…12 Finally, acid hydrolysis of 1 obtained D-glucose and D-apiose, which were confirmed by HPLC analysis of their thiocarbamoylthiazolidine derivatives by comparison with authentic sugars. 13,14 Consequently, the structure of 1 was established and named derriacuminoside A (Supplemental Figures S1-S7).…”

“…Later, the presence of D-glucose and D-apiose in both 2 and 3 were also confirmed by acid hydrolysis, HPLC analysis of the thiocarbamoylthiazolidine products, and comparison with authentic sugars. 13,14 Thus, the structures of 2 and 3 were established and named as derriacuminosides B and C, respectively (Supplemental Figures S8-S18).…”

“…The NO assay was based on Griess reaction, as previously described. 14,15 Compounds 1 - 3 inhibited NO production with IC 50 values of 26.54 ± 3.08, 26.35 ± 1.37, and 49.71 ± 2.93 µM, respectively, compared to the positive control N G -methyl-L-arginine acetate salt (L-NMMA), which had an IC 50 value of 35.56 ± 2.98 µM. Therefore, compounds 1 - 3 could be active anti-inflammatory constituents and partly responsible for the anti-inflammatory property of D elliptica .…”