Three new combinations from the Cryptococcus laurentii complex: Cryptococcus aureus, Cryptococcus carnescens and Cryptococcus peneaus

Takashima, Masako; Sugita, Takashi; Saitô, Takako; Nakase, Takashi

doi:10.1099/ijs.0.02498-0

Cited by 72 publications

(64 citation statements)

References 29 publications

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“…The Cryptococcus laurentii species complex (15 species) can be divided into two phylogenetically distinct groups [30]. Based on the sequences determined by Takashima et al [30], our assay can differentiate among all the species currently included withinphylogenetic group I (e.g.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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Pyrosequencing was used to identify 133 isolates of clinically relevant non-dematiaceous yeasts. These included 97 ATCC strains (42 type strains), 7 UAMH strains, and 29 clinical isolates. Isolates belonged to the following genera: Candida (18 species), Trichosporon (10), Cryptococcus (7), Malassezia (3), Rhodotorula (2), Geotrichum (1), Blastoschizomyces (1) and Kodamaea (1). Amplicons were obtained of a hypervariable ITS region and analyzed using Pyrosequencing technology. The data were evaluated by a BLAST search against the GenBank database and correlated with data obtained by conventional cycle sequencing of the ITS1-5.8S-ITS2 region. Cycle sequencing identified (78.9%) if the isolates to the species level. Pyrosequencing technology identified 69.1%. In 90.1% of all the strains tested, identification results of both sequencing methods were identical. Most Candida isolates can be identified to the species level by pyrosequencing. Trichosporon species and some Cryptococcus cannot be differentiated at the species level. Pyrosequencing can be used for the reliable identification of most commonly isolated nondematiaceous yeasts, with a reduction of cost per identification compared to conventional sequencing.

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Section: Discussionmentioning

confidence: 99%

“…Based on the sequences determined by Takashima et al [30], our assay can differentiate among all the species currently included withinphylogenetic group I (e.g. C. flavescens, C. aureus, C. laurentii ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Montero

Shea

Jones

et al. 2008

Eur J Clin Microbiol Infect Dis

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“…An example is [C. laurentii] CBS 8645, recently reclassified by Takashima et al (2003) as C. flavescens. The same happens with some strains identified as C. flavescens that appear in the C. terrestris clade.…”

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confidence: 99%

“…Cryptococcus carnescens CBS 973 T was designated the outgroup in this analysis. Given differences in reported sequences for strain CBS 8645 (Sugita et al, 2000;Takashima et al, 2003;and GenBank), the sequence represented by GenBank accession number AB087253 was used for the sake of consistency. Type strains are highlighted in bold.…”

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confidence: 99%

Cryptococcus terrestris sp. nov., a tremellaceous, anamorphic yeast phylogenetically related to Cryptococcus flavescens

Crestani

Landell

Faganello

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Cryptococcus terrestris sp. nov. (Basidiomycota, Agaricomycotina, Tremellomycetes, Tremellales) is typified by CJDX4 Y23 T (5CBS 10810 T 5NRRL Y-48451 T ), isolated from forest soil in Oklahoma, USA. This species is most readily identified by the sequence of the D1/D2 domain region of the 26S rDNA and ITS (internal transcribed spacer) region. Additional strains from Oklahoma (C107DX4 Y11 5CBS 10813 5NRRL Y-48452) and Brazil (Ep11c 5CBS 10812 5NRRL Y-48454; 56e 5CBS 10811 5NRRL Y-48453) either had identical sequences or differed minimally. C. terrestris differs physiologically from the most closely related species, Cryptococcus flavescens, by the weak or delayed assimilation of ribose and salicin, and differs from Cryptococcus aureus by the utilization of nitrate and nitrite and growth in vitamin-free medium.Members of the genus Cryptococcus are found in a wide variety of habitats, and are difficult to identify by traditional methods. The genus is polyphyletic and is distributed throughout the class Tremellomycetes, with representatives in all orders (Tremellales, Filobasidiales and Cystofilobasidiales) (Scorzetti et al., 2002;Hibbett et al., 2007). Independent yeast surveys in Oklahoma (USA) and Rio Grande do Sul (Brazil) produced four isolates with very similar physiological profiles and sequences of the D1/D2 domain of the 26S rDNA and internal transcribed spacer (ITS) region. Sequences differed significantly from those of any previously described yeast species, and the isolates formed a discrete clade close to Cryptococcus flavescens. This study deals with the description of this novel species, for which the name Cryptococcus terrestris sp. nov. is proposed.The Oklahoman strains CJDX4 Y23 T and C107DX4 Y11 were isolated from a sandy loam (sand 67.5 %, silt 28.7 %, clay 3.8 %) Konawa series soil collected in the rhizosphere of a mixed hardwood (mainly Quercus spp.) forest in Payne County, Oklahoma, on 6 July 2006 (35 u 59.9869 N 97 u 05.0899 W) and 27 January 2007 (35u 59.9499 N 97u 05.0509 W), respectively. A subsample of the surface to 2 cm layer was incubated overnight in 50 ml M3C (Vishniac, 2002) plus an antibiotic mixture on a New Brunswick Gyrotary shaker and then sampled by spreading 0.1 ml of appropriate dilutions on M3C plus antibiotic agar. The antibiotic mixture included 160 mg penicillin G potassium salt and 100 mg streptomycin sulfate ml 21 for the July sample, augmented by the addition of 32 mg gentamicin sulfate ml 21 for the January sample. The Brazilian strains Ep11c and 56e were isolated during an epidemiological study of yeasts associated with a case of cryptococcal meningitis in a worker from a timber factory at Cachoeira do Sul in Rio Grande do Sul state in southern Brazil (30 u 00.4709 S 52 u 54.8659 W). The factory dealt mostly with wood of Pinus spp. trees, but also processed Ficus and some Eucalyptus trees. Both strains were collected within the factory area. Strain Ep11c was isolated on 26 September 2005 from dry pigeon droppings that were lying on the grass. Strain 56e was isolated ...

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“…Sequences for phylogenetic analysis were aligned using CLUSTAL_X 1.83 (Thompson et al, 1997). (Sugita et al, 2000;Takashima et al, 2003). Bootstrap percentage (1000 replicates) of 50 % or greater are shown.…”

Section: Rdna Sequence Analysesmentioning

confidence: 99%

Cryptococcus anemochoreius sp. nov., a novel anamorphic basidiomycetous yeast isolated from the atmosphere in central South Africa

Pohl

Kock

Wyk

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A novel yeast strain, CBS 10258 T , was isolated from the atmosphere in central South Africa. Sequence analysis of the D1/D2 domain and internal transcribed spacer region of the novel strain indicates that it represents a novel species within the Cryptococcus laurentii complex. Phylogenetic analyses based on the D1/D2 domain revealed that the novel strain occupies a relatively isolated position within this complex with Papiliotrema bandonii, Cryptococcus perniciosus, Cryptococcus nemorosus and Cryptococcus sp. CBS 8363 being the closest relatives. However, the novel strain could be distinguished from related species by standard physiological tests including the inability to assimilate rhamnose, methyl a-D-glucoside, salicin, lactose, erythritol, ribitol, xylitol, citrate and ethanol. In addition, no extracellular starch production was observed and the isolate was able to grow in the absence of additional vitamins. On the basis of these results, we suggest that the new strain represents a novel species for which the name Cryptococcus anemochoreius sp. nov. is proposed [type strain CBS 10258 T (=NRRL Y-27920 T )].

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Three new combinations from the Cryptococcus laurentii complex: Cryptococcus aureus, Cryptococcus carnescens and Cryptococcus peneaus

Cited by 72 publications

References 29 publications

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Evaluation of Pyrosequencing® technology for the identification of clinically relevant non-dematiaceous yeasts and related species

Cryptococcus terrestris sp. nov., a tremellaceous, anamorphic yeast phylogenetically related to Cryptococcus flavescens

Cryptococcus anemochoreius sp. nov., a novel anamorphic basidiomycetous yeast isolated from the atmosphere in central South Africa

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