Three New Anti-Rotavirus Quinoline Alkaloids from the Whole Plant of Thalictrum Glandulosissimum

Abstract

one (3) were isolated from the whole plants of Thalictrum glandulosissimum.Their structures were elucidated by spectroscopic methods, including extensive 1 H, 13 C, and 2D-NMR techniques. Compounds 1-3 were also tested for their anti-rotavirus activity. Compounds 1-3 exhibited potent anti-rotavirus activity with therapeutic index (TI) valves of 17.6, 15.0, and 23.6 respectively.The genus Thalictrum belongs to the subfamily Thalictroideae of Ranunculaceae and consists of the subgenera Thalictrum and Lecoyerium. 1,2 This genus has around 200 species, which are distributed in Asia, Europe, Africa, North America, and South America. Around 67 species are recorded in the flora of China, and the majority of which are found in Southwest China. At least 43 species of this genus are used medicinally in China. 2,3 This genus is also well known for its diverse pharmacological activities, including anti-tumor, anti-virus, anti-microbial, anti-tuberculosis, anti-inflammatory, anti-malarial activities, and the like, 4 and the main active ingredients found in this genus are alkaloids, 5-7 flavonoids, 8,9 triterpenoid saponins, 10,11 lithospermoside, 12 and the like.Thalictrum glandulosissimum is a herbaceous plant of Thalictrum genus with short rhizome and many thick fibrous roots. It distributed in Dali, Heqing, and Binchuan Prefecture, Yunnan Province, and grows on the hillside grassland at an altitude of 2500 meters. 2 The whole plants of T. glandulosissimum are used to treat some diseases, such as enteritis, dysentery, jaundice and throat by Bai nationality people in Yunnan Province of China, and the alkaloids are the main active components in this plants. 3,7,13,14 In the