Three Interconvertible Forms of Glucose-6-Phosphate Dehydrogenase in Human Liver and Erythrocyte

Abstract

The separation of three electrophoretically distinct subcomponents of normal G6PD (type B^+) in human liver and erythrocyte has been achieved by means of disc electrophoresis. Molecular weights of these forms were found to be similar, and their resolution on disc electrophoresis appeared to be mainly due to the difference in net charge. The three forms were demonstrated to be interconvertible by treatment with sulfhydryl reagents

Glucose‐6‐Phosphate Dehydrogenases

“…Such oxidation-reduction reactions of cysteine residues have been implicated in generating the three-band pattern observed on polyacrylamide gel electrophoresis of G6PD from human erythrocytes and other tissues (88,374) and rat liver (375); similar results with rat mammary GGPD ( 15 1,152) are described above.…”

Microsomal Hexose-6-Phosphate Dehydrogenase in Injured Liver

“…Vertical disc electrophoresis on polyacrylamide gels and cellulose acetate (Cellogel) electrophoresis of the enzymes were carried out according to the re ported procedures (17,18). Enzyme activity was detected by staining the electrophoresed strips and gels with 2.5 mmol/1 Glc-6-P or Gal-6-P as described earlier (17,18).…”

“…Enzyme activity was detected by staining the electrophoresed strips and gels with 2.5 mmol/1 Glc-6-P or Gal-6-P as described earlier (17,18).…”

“…Louis., Mo. Other reagents used were purchased from sources previously described (17)(18)(19)(20)(21).…”