1993
DOI: 10.1002/jsfa.2740610216
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Three extraction methods for determination of lipids in fish meal: Evaluation of a hexane/isopropanol method as an alternative to chloroform‐based methods

Abstract: Three different extraction methods were compared for the determination of lipids in both oxidised and antioxidant-stabilised brown fish meal. The three methods under investigation were those of Smith-Ambrose-Knob1 using chloroform/methanol/water, Bligh and Dyer also using chloroform/methanol/ water but monophasic, and Hara and Radin using hexane/isopropanol. The fat content, determined gravimetrically, was significantly lower (P < 0.05) when

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Cited by 60 publications
(52 citation statements)
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“…First, ethanol and isopropanol are less polar than methanol in the BD method [as observed by Zief and Kiser (18)]; second, the solubility of polar lipids is low in hydrocarbon solvents, such as hexane, when compared with their solubility in chloroform (15). When the results obtained using the chloroform/ methanol/water solvent system were compared with that of hexane/propan-2-ol/water (19), the former had a higher extraction yield.…”
Section: Resultsmentioning
confidence: 90%
See 1 more Smart Citation
“…First, ethanol and isopropanol are less polar than methanol in the BD method [as observed by Zief and Kiser (18)]; second, the solubility of polar lipids is low in hydrocarbon solvents, such as hexane, when compared with their solubility in chloroform (15). When the results obtained using the chloroform/ methanol/water solvent system were compared with that of hexane/propan-2-ol/water (19), the former had a higher extraction yield.…”
Section: Resultsmentioning
confidence: 90%
“…Among the methods studied were BD and HR, and the HR method was found to extract the smallest amount of polar lipids from meat samples with medium and high fat contents (19).…”
Section: Resultsmentioning
confidence: 99%
“…It has been shown that chloroform/methanol extraction could result in higher overall lipid yields than other solvents or solvent pairs by dissolving more polar substances, such as polar lipids (Gunnlaugsdottir and Ackman, 1993). In order to reduce the solvent toxicity from chloroform, alternative solvents or solvent pairs have also been tested, such as methyltert-butyl ether (Matyash et al, 2008), hexane (Danielewicz et al, 2011), and hexane/isopropanol (Hara and Radin, 1978).…”
Section: Chemical Characterization Extraction and Fractionation Of LImentioning
confidence: 99%
“…Nos últimos anos diversos pesquisadores realizaram trabalhos 16,17,[19][20][21][22][23] em que o objetivo foi encontrar o método mais eficiente para a obtenção da fração lipídica desejada e determinar sua composição. Além disso, também buscaram métodos que não alterassem a qualidade do óleo e utilizassem solventes menos tóxicos que a mistura clorofórmio e metanol.…”
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