Three‐dimensional ultrastructural analysis and histomorphometry of collagen bundles in the periodontal ligament using focused ion beam/scanning electron microscope tomography

Hirashima, Shingo; Ohta, Keisuke; Kanazawa, Tomonoshin; Okayama, Satoko; Togo, Akinobu; Miyazono, Yoshihiro; Kusukawa, Jingo; Nakamura, Kei‐ichiro

doi:10.1111/jre.12592

Cited by 17 publications

(20 citation statements)

References 33 publications

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“…The experimental results reported by Natali et al show a softening phenomenon of the tissue under load repetition that may be caused by rearrangement of fibres . In the nonloading state, the fibrous network is partially disorganized with interweaved collagen fibres . This organization leads to a stiffer behaviour of the matrix than if the fibres were not interconnected.…”

Section: Introductionmentioning

confidence: 99%

“…16,26,30 In the nonloading state, the fibrous network is partially disorganized with interweaved collagen fibres. 31 This organization leads to a stiffer behaviour of the matrix than if the fibres were not interconnected. When the tissue is stretched, the local movement of the collagen fibres causes the fibrous network to become untangled, resulting in a softening phenomenon 31 that has been observed in other soft tissues, such as the arteries, 32 veins, 33 vaginal tissues, 34 and oesophageal tissues.…”

Section: Introductionmentioning

confidence: 99%

“…This organization leads to a stiffer behaviour of the matrix than if the fibres were not interconnected. When the tissue is stretched, the local movement of the collagen fibres causes the fibrous network to become untangled, resulting in a softening phenomenon that has been observed in other soft tissues, such as the arteries, veins, vaginal tissues, and oesophageal tissues . No numerical study of PDL damage has considered softening effects due to fibre alignment or fibrous network failure.…”

Section: Introductionmentioning

confidence: 99%

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A porous fibrous hyperelastic damage model for human periodontal ligament: Application of a microcomputerized tomography finite element model

Ortún‐Terrazas

Cegoñino

Santana‐Penín

et al. 2019

Numer Methods Biomed Eng

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The periodontal ligament (PDL) is a soft biological tissue that connects the tooth with the trabecular bone of the mandible. It plays a key role in load transmission and is primarily responsible for bone resorption and most common periodontal diseases. Although several numerical studies have analysed the biomechanical response of the PDL, most did not consider its porous fibrous structure, and only a few analysed damage to the PDL. This study presents an innovative numerical formulation of a porous fibrous hyperelastic damage material model for the PDL. The model considers two separate softening phenomena: fibre alignment during loading and fibre rupture. The parameters for the material model characterization were fitted using experimental data from the literature. Furthermore, the experimental tests used for characterization were computationally modelled to verify the material parameters. A finite element model of a portion of a human mandible, obtained by microcomputerized tomography, was developed, and the proposed constitutive model was implemented for the PDL. Our results confirm that damage to the PDL may occur mainly because of overpressure of the interstitial fluid, while large forces must be applied to damage the PDL fibrous network. Moreover, this study clarifies some aspects of the relationship between PDL damage and the bone remodelling process.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A porous fibrous hyperelastic damage model for human periodontal ligament: Application of a microcomputerized tomography finite element model

Ortún‐Terrazas

Cegoñino

Santana‐Penín

et al. 2019

Numer Methods Biomed Eng

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“…The human PDL is known as a heterogenous connective tissue mainly composed of fiber networks and bundles with different orientations and locations longitudinally. The PDL fibers around the cervical part of the tooth root are oriented horizontally, while the PDL in the middle part of the root consists of the oblique fibers [ 24 ]. An earlier study reported that as a result of sawing, the disruption of oblique fibers in the PDL within the transverse section can cause inevitable errors [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Frequency-related viscoelastic properties of the human incisor periodontal ligament under dynamic compressive loading

Zhao

et al. 2020

PLoS ONE

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Studies concerning the mechanical properties of the human periodontal ligament under dynamic compression are rare. This study aimed to determine the viscoelastic properties of the human periodontal ligament under dynamic compressive loading. Ten human incisor specimens containing 5 maxillary central incisors and 5 maxillary lateral incisors were used in a dynamic mechanical analysis. Frequency sweep tests were performed under the selected frequencies between 0.05 Hz and 5 Hz with a compression amplitude that was 2% of the PDL’s initial width. The compressive strain varied over a range of 4%-8% of the PDL’s initial width. The storage modulus, ranging from 28.61 MPa to 250.21 MPa, increased with the increase in frequency. The loss modulus (from 6.00 MPa to 49.28 MPa) also increased with frequency from 0.05 Hz– 0.5 Hz but remained constant when the frequency was higher than 0.5 Hz. The tan δ showed a negative logarithmic correlation with frequency. The dynamic moduli and the loss tangent of the central incisor were higher than those of the lateral incisor. This study concluded that the human PDL exhibits viscoelastic behavior under compressive loadings within the range of the used frequency, 0.05 Hz– 5 Hz. The tooth position and testing frequency may have effects on the viscoelastic properties of PDL.

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“…The main role of the PDL is the anchoring of the tooth to the surrounding mandibular/alveolar bone and providing a space to allow tooth movement within the bone socket. This is mediated by bundles of collagen fibers ending as Sharpey’s fibers embedded in the cementum on one side and bone on the other ( Figures 3D,E ; Ho et al, 2007 ; Lee et al, 2015 ; Hirashima et al, 2020a ). Based on immunoelectron microscopy, Huang et al (1991) have proposed that periodontal and Sharpey’s fibers consist in co-fibrils of type I and type III collagens, which is supported by the similar flexibility of alfa-1 chains in both collagen types and their possible involvement in elastic energy storage during stretching ( Silver et al, 2002 ).…”

Section: Mesenchymes Specification and Differentiation During Developmentioning

confidence: 99%

Formation and Developmental Specification of the Odontogenic and Osteogenic Mesenchymes

Švandová

Peterková

Matalová

et al. 2020

Front. Cell Dev. Biol.

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Within the mandible, the odontogenic and osteogenic mesenchymes develop in a close proximity and form at about the same time. They both originate from the cranial neural crest. These two condensing ecto-mesenchymes are soon separated from each other by a very loose interstitial mesenchyme, whose cells do not express markers suggesting a neural crest origin. The two condensations give rise to mineralized tissues while the loose interstitial mesenchyme, remains as a soft tissue. This is crucial for proper anchorage of mammalian teeth. The situation in all three regions of the mesenchyme was compared with regard to cell heterogeneity. As the development progresses, the early phenotypic differences and the complexity in cell heterogeneity increases. The differences reported here and their evolution during development progressively specifies each of the three compartments. The aim of this review was to discuss the mechanisms underlying condensation in both the odontogenic and osteogenic compartments as well as the progressive differentiation of all three mesenchymes during development. Very early, they show physical and structural differences including cell density, shape and organization as well as the secretion of three distinct matrices, two of which will mineralize. Based on these data, this review highlights the consecutive differences in cell-cell and cell-matrix interactions, which support the cohesion as well as mechanosensing and mechanotransduction. These are involved in the conversion of mechanical energy into biochemical signals, cytoskeletal rearrangements cell differentiation, or collective cell behavior.

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Three‐dimensional ultrastructural analysis and histomorphometry of collagen bundles in the periodontal ligament using focused ion beam/scanning electron microscope tomography

Cited by 17 publications

References 33 publications

A porous fibrous hyperelastic damage model for human periodontal ligament: Application of a microcomputerized tomography finite element model

A porous fibrous hyperelastic damage model for human periodontal ligament: Application of a microcomputerized tomography finite element model

Frequency-related viscoelastic properties of the human incisor periodontal ligament under dynamic compressive loading

Formation and Developmental Specification of the Odontogenic and Osteogenic Mesenchymes

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