Three‐dimensional structure of the wheat β‐amylase Tri a 17, a clinically relevant food allergen

Hofer, Gerhard; Wieser, Sandra; Bogdos, Michael K.; Gattinger, Pia; Nakamura, Ryosuke; Ebisawa, Motohiro; Mäkelä, Mika J.; Papadopoulos, Nikolaos G.; Valenta, Rudolf; Keller, Walter

doi:10.1111/all.13696

Cited by 15 publications

(10 citation statements)

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“…The molecular interaction of IgE antibodies with the allergen occurs with linear (continuous) or conformational (discontinuous) epitopes. [32][33][34][35] High-throughput overlapping peptide scanning approaches based on the primary amino acid sequence of reported allergens focus on stable linear components of allergens, which are more frequent in food allergens. 24,[36][37][38] Evidence suggests that some determinants of IgE recognition define the strength of a clinical response: abundance, epitope diversity, 39 epitope proximity, and overlap of IgE epitope recognition with antibodies from different isotypes, in particular of IgG4 and IgA.…”

Section: Novel Data On Clinical Markers and Biomarkers Of Food Allergymentioning

confidence: 99%

“…The molecular interaction of IgE antibodies with the allergen occurs with linear (continuous) or conformational (discontinuous) epitopes . High‐throughput overlapping peptide scanning approaches based on the primary amino acid sequence of reported allergens focus on stable linear components of allergens, which are more frequent in food allergens .…”

Section: Introductionmentioning

confidence: 99%

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Recent developments and highlights in food allergy

Eiwegger

Hung

Diego

et al. 2019

Allergy

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The achievement of long-lasting, safe treatments for food allergy is dependent on the understanding of the immunological basis of food allergy. Accurate diagnosis is essential for management. In recent years, data from oral food challenges have revealed that routine allergy testing is poor at predicting clinical allergy for tree nuts, almonds in particular. More advanced antigen-based tests including component-resolved diagnostics and epitope reactivity may lead to more accurate diagnosis and selection of therapeutic intervention. Additional diagnostic accuracy may come from cellular tests such as the basophil activation test or mast cell approaches. In the context of clinical trials, cellular tests have revealed specific T-cell and B-cell populations that are more abundant in food-allergic individuals with distinct mechanistic features.Awareness of clinical markers, such as the ability to eat baked forms of milk and egg, continues to inform the understanding of natural tolerance development.Mouse models have allowed for investigation into multiple mechanisms of food allergy including modification of epithelial metabolism, and the induction of regulatory cell subsets and the microbiome. Increasing numbers of children who underwent food immunotherapy enlarged the body of evidence on mechanisms and predictors of treatment success. Experimental immunological markers in conjunction with clinical determinants such as lower age and lower initial specific IgE appear to be of benefit.More research on the optimal dose, preparation, and route of application integrating a high-level safety and efficacy is demanded. Alternatively, biologics blocking TSLP, IL-33, IL-4 and IL-13, or IgE may help to achieve that. K E Y W O R D Sallergy treatment, biologics, food allergy, immune tolerance, immunotherapy | 2357 EIWEGGER Et al.

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Section: Novel Data On Clinical Markers and Biomarkers Of Food Allergymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent developments and highlights in food allergy

Eiwegger

Hung

Diego

et al. 2019

Allergy

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“…Therefore, the IgE binding capacity of Chinese wheat was mainly determined by rRNA N-glycosidase and β-amylase in soluble protein. 18 …”

Section: Resultsmentioning

confidence: 99%

Allergenicity assessment and allergen profile analysis of different Chinese wheat cultivars

Wang

Weng

Zhu

et al. 2021

World Allergy Organization Journal

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Backgrounds As one of the most important cereals, wheat ( Triticum aestivum ) can cause severe allergic reactions, such as baker's asthma, allergic rhinitis, and atopic dermatitis. A growing number of people are developing allergies to Chinese wheat; however, only a few wheat cultivars have been screened on allergenicity in China. Objective The aim of the present study was to assess the allergenicity of different Chinese wheat cultivars and characterize wheat allergen profiles of patients with allergic rhinitis. Methods We determined protein (soluble protein, gliadin, and glutenin) composition in Chinese wheat by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the immunoglobulin E (IgE) binding capacity by enzyme-linked immunosorbent assay (ELISA) and Western blot using 10 positive sera from wheat allergy patients. We identified 5 gel bands with significant IgE binding capacity using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results Soluble protein, albumin, and globulin, showed the highest allergenicity, followed by gliadin, while glutenin only had slight allergenicity. In soluble protein, 5 protein bands with molecular weights of 27, 28, 53, 58, and 62 kDa showed very significant allergenicity. Meanwhile, the relative abundances of 28 kDa-protein and 58 kDa-protein were significantly positively correlated with the IgE-binding capacity of Chinese wheat cultivars, which were identified as rRNA N-glycosidase and β-amylase, respectively, among other proteins in those highly complex gel bands. Conclusion and clinical relevance 28 kDa-protein (rRNA N-glycosidase) and 58 kDa-protein (β-amylase) were speculated to be the main allergens of Chinese wheat causing baker's asthma and allergic rhinitis. These results provide new insights into the prevention and treatment of wheat allergy and the development of hypoallergenic wheat products, whose clinical significance is worth further evaluation.

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“…At present, most of the wheat allergens that have been identified belong to the α-amylase/trypsin inhibitor family, and there are 18 species: Tri a 12, Tri a 14, Tri a 17-21, Tri a 25-28, Tri a 36-37, and Tri a 41-45. Among them, Tri a 14, Tri a 19, and Tri a 36 are the major allergens [124][125][126].…”

Section: Wheatmentioning

confidence: 99%

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

Hong

Pan

Xie

et al. 2021

Foods

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Food allergies have seriously affected the life quality of some people and even endangered their lives. At present, there is still no effective cure for food allergies. Avoiding the intake of allergenic food is still the most effective way to prevent allergic diseases. Therefore, it is necessary to develop rapid, accurate, sensitive, and reliable analysis methods to detect food allergens from different sources. Aptamers are oligonucleotide sequences that can bind to a variety of targets with high specificity and selectivity, and they are often combined with different transduction technologies, thereby constructing various types of aptamer sensors. In recent years, with the development of technology and the application of new materials, the sensitivity, portability, and cost of fluorescence sensing technology have been greatly improved. Therefore, aptamer-based fluorescence sensing technology has been widely developed and applied in the specific recognition of food allergens. In this paper, the classification of major allergens and their characteristics in animal and plant foods were comprehensively reviewed, and the preparation principles and practical applications of aptamer-based fluorescence biosensors are summarized. In addition, we hope that this article can provide some strategies for the rapid and sensitive detection of allergens in food matrices.

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Three‐dimensional structure of the wheat β‐amylase Tri a 17, a clinically relevant food allergen

Cited by 15 publications

References 10 publications

Recent developments and highlights in food allergy

Recent developments and highlights in food allergy

Allergenicity assessment and allergen profile analysis of different Chinese wheat cultivars

Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices

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