Three-dimensional reconstruction of brain capillaries from frozen serial sections

Wiederhold, Karl‐Heinz; Bielser, W.; Schulz, Ursula G.; Veteau, M.-J.; Hunziker, O.

doi:10.1016/0026-2862(76)90049-2

Cited by 41 publications

(11 citation statements)

References 9 publications

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“…A three-dimensional reconstruction of brain capillaries from frozen serial sections of feline temporo-parietal Suprasylvian–Gyrus revealed an average capillary diameter of 4.68 ± 0.92 μm, total capillary length of 42.55 cm/mm 2 of brain tissue, and a capillary volume of 2.8%. 33 …”

Section: Two Factors Affect Brain Signal In Pet Studies Of P-gp Densitymentioning

confidence: 99%

Factors That Limit Positron Emission Tomography Imaging of P-Glycoprotein Density at the Blood–Brain Barrier

et al. 2013

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Efflux transporters located at the blood–brain barrier, such as P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP), regulate the passage of many drugs in and out of the brain. Changes in the function and density of these proteins, in particular P-gp, may play a role in several neurological disorders. Several radioligands have been developed for measuring P-gp function at the blood–brain barrier of human subjects with positron emission tomography (PET). However, attempts to measure P-gp density with radiolabeled inhibitors that bind to these proteins in vivo have not thus far provided useful, quantifiable PET signals. Herein, we argue that not only the low density of transporters in the brain as a whole but also their very high density in brain capillaries act to lower the concentration of ligand in the plasma and thereby contribute to absent or low signals in PET studies of P-gp density. Our calculations, based on published data and theoretical approximations, estimate that whole brain densities of many efflux transporters at the blood–brain barrier range from 0.04 to 5.19 nM. We conclude that the moderate affinities (>5 nM) of currently labeled inhibitors may not allow measurement of efflux transporter density at the blood–brain barrier, and inhibitors with substantially higher affinity will be needed for density imaging of P-gp and other blood–brain barrier transporters.

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Section: Two Factors Affect Brain Signal In Pet Studies Of P-gp Densitymentioning

confidence: 99%

Factors That Limit Positron Emission Tomography Imaging of P-Glycoprotein Density at the Blood–Brain Barrier

et al. 2013

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“…The overall organization of the human cortical vascular system was studied by Duvernoy et al (7). Wiederhold et al (35) constructed a three-dimensional physical model of the capillary network in a region of cat cerebral cortex, based on frozen serial sections. Motti et al (26) used scanning electron microscopy (SEM) to study corrosion casts of the microvasculature in the superficial cortex of the rat.…”

Section: Introductionmentioning

confidence: 99%

Theoretical Simulation of Oxygen Transport to Brain by Networks of Microvessels: Effects of Oxygen Supply and Demand on Tissue Hypoxia

Secomb¹,

HSU²,

BEAMER³

et al. 2000

Microcirculation

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“…Three-dimensional reconstruction of biological structures has been used to demonstrate brain capil-laries by Wiederhold et al (1976); vertebrae by Hakim and King (1978); germinal epithelium distribution patterns in the Java monkey by Dietrich et al (1986); and veil cell distribution around cutaneous microvessels by Braverman et al (1986).…”

Section: Introductionmentioning

confidence: 99%

Microcomputer reconstruction of pancreaticobiliary embryogenesis

1989

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The origin, approximation, and fusion of the dorsal and ventral pancreatic anlage were reconstructed using new microcomputer methodology. Representative human embryos of Carnegie stage [15][16][17] were studied by digitizing serial contours and creating three-dimensional computer models. Solid surface color displays showing multiple views of each reconstruction demonstrated developmental relationships and changes between these three critical stages. This exercise demonstrates the power of this new technology for clarifying morphological concepts in research and education.

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Three-dimensional reconstruction of brain capillaries from frozen serial sections

Cited by 41 publications

References 9 publications

Factors That Limit Positron Emission Tomography Imaging of P-Glycoprotein Density at the Blood–Brain Barrier

Factors That Limit Positron Emission Tomography Imaging of P-Glycoprotein Density at the Blood–Brain Barrier

Theoretical Simulation of Oxygen Transport to Brain by Networks of Microvessels: Effects of Oxygen Supply and Demand on Tissue Hypoxia

Microcomputer reconstruction of pancreaticobiliary embryogenesis

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