Three-Dimensional Printing of Cell Exclusion Spacers (CES) for Use in Motility Assays

Boyer, Christen J.; Ballard, David H.; Yun, J. Winny; Xiao, Adam Y.; Weisman, Jeffery A.; Barzegar, Mansoureh

doi:10.1007/s11095-018-2431-4

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…These types of customized cell culture inserts therefore have major advantages for research groups with limited funding and access to consumer or commercial grade 3D printers. 3D printing represents an economical and practical tool for ad hoc, de novo, or template-based creation of 3D printed constructs to aid with tissue engineering, cell cultures, and other laboratory experiments [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

High-throughput scaffold-free microtissues through 3D printing

et al. 2018

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BackgroundThree-dimensional (3D) cell cultures and 3D bioprinting have recently gained attention based on their multiple advantages over two-dimensional (2D) cell cultures, which have less translational potential to recapitulate human physiology. 3D scaffold supports, cell aggregate systems and hydrogels have been shown to accurately mimic native tissues and support more relevant cell-cell interactions for studying effects of drugs and bioactive agents on cells in 3D. The development of cost-effective, high-throughput and scaffold-free microtissue assays remains challenging. In the present study, consumer grade 3D printing was examined as a fabrication method for creation of high-throughput scaffold-free 3D spheroidal microtissues.ResultsConsumer grade 3D printing was capable of forming 96-well cell culture inserts to create scaffold-free microtissues in liquid suspensions. The inserts were seeded with human glioblastoma, placental-derived mesenchymal stem cells, and intestinal smooth muscle cells. These inserts allowed for consistent formation of cell density-controllable microtissues that permit screening of bioactive agents.ConclusionA variety of different cell types, co-cultures, and drugs may be evaluated with this 3D printed microtissue insert. It is suggested that the microtissue inserts may benefit 3D cell culture researchers as an economical assay solution with applications in pharmaceuticals, disease modeling, and tissue-engineering.

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Section: Discussionmentioning

confidence: 99%

High-throughput scaffold-free microtissues through 3D printing

et al. 2018

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“…Some researchers used TinkerCAD to print for the printing of necessary equipment needed for biofabrication. For example, it was used for modeling of inserts in papers[ 56 , 57 ]. In Ivanov and Grabowska[ 58 ], the design of the mold maker was provided with TinkerCAD, the authors of Yang et al .…”

Section: Software For Bioprintingmentioning

confidence: 99%

Software for Bioprinting

Pakhomova¹,

Popov²,

Maltsev³

et al. 2024

IJB

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The bioprinting of heterogeneous organs is a crucial issue. To reach the complexity of such organs, there is a need for highly specialized software that will meet all requirements such as accuracy, complexity, and others. The primary objective of this review is to consider various software tools that are used in bioprinting and to reveal their capabilities. The sub-objective was to consider different approaches for the model creation using these software tools. Related articles on this topic were analyzed. Software tools are classified based on control tools, general computer-aided design (CAD) tools, tools to convert medical data to CAD formats, and a few highly specialized research-project tools. Different geometry representations are considered, and their advantages and disadvantages are considered applicable to heterogeneous volume modeling and bioprinting. The primary factor for the analysis is suitability of the software for heterogeneous volume modeling and bioprinting or multimaterial three-dimensional printing due to the commonality of these technologies. A shortage of specialized suitable software tools is revealed. There is a need to develop a new application area such as computer science for bioprinting which can contribute significantly in future research work.

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“…As it is difficult to mimic 3D cell migration in laboratory setup, this phenomenon is most often studied in an in vitro 2-dimensional (2D) cell cultures ( Riahi et al, 2012 ; Stamm et al, 2016 ). 2D studies provide conceptual evidence and understanding of the cell migration rate and directionality ( Riahi et al, 2012 ; Stamm et al, 2016 ; Grada et al, 2017 ; Boyer et al, 2018 ; Acosta et al, 2022 ), but significant differences exist between 2D and 3D cell migration, so caution must be exerted when interpreting 2D migration results. 2D cell migration studies are often associated with absence and presence of additional chemical entities such as cell migration promoters (e.g.,: growth factors) or cell migration inhibitors (e.g.,: potential anticancer drug candidates).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, in an attempt to improve this experimental technique, other types of inserts have been introduced, which work on the principle similar to the ibidi GmbH inserts. The most recent advance in such inserts were reported by Acosta et al and Boyer et al Even though their inserts yielded reproducible results, the technology still has some notable limitations, mentioned in Table 2 ( Boyer et al, 2018 ; Acosta et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

3D printed inserts for reproducible high throughput screening of cell migration

Joshi,

Madhusudanan,

Mijakovic

2023

Front. Cell Dev. Biol.

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Cell migration is a fundamental and complex phenomenon that occurs in normal physiology and in diseases like cancer. Hence, understanding cell migration is very important in the fields of developmental biology and biomedical sciences. Cell migration occurs in 3 dimensions (3D) and involves an interplay of migrating cell(s), neighboring cells, extracellular matrix, and signaling molecules. To understand this phenomenon, most of the currently available techniques still rely on 2-dimensional (2D) cell migration assay, also known as the scratch assay or the wound healing assay. These methods suffer from limited reproducibility in creating a cell-free region (a scratch or a wound). Mechanical/heat related stress to cells is another issue which hampers the applicability of these methods. To tackle these problems, we developed an alternative method based on 3D printed biocompatible cell inserts, for quantifying cell migration in 24-well plates. The inserts were successfully validated via a high throughput assay for following migration of lung cancer cell line (A549 cell line) in the presence of standard cell migration promoters and inhibitors. We also developed an accompanying image analysis pipeline which demonstrated that our method outperforms the state-of-the-art methodologies for assessing the cell migration in terms of reproducibility and simplicity.

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Three-Dimensional Printing of Cell Exclusion Spacers (CES) for Use in Motility Assays

Abstract: Cell migration distances were significantly reduced by Cyto-D, supporting the use of 3D printing for cell exclusion assays. 3D printed CES have great potential for studying cell motility, migration/invasion, and complex multi-cell interactions.

Cited by 6 publications

References 17 publications

High-throughput scaffold-free microtissues through 3D printing

High-throughput scaffold-free microtissues through 3D printing

Software for Bioprinting

3D printed inserts for reproducible high throughput screening of cell migration

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