Three-Dimensional Printed Titanium Scaffolds Enhance Osteogenic Differentiation and New Bone Formation by Cultured Adipose Tissue-Derived Stem Cells Through the IGF-1R/AKT/Mammalian Target of Rapamycin Complex 1 (mTORC1) Pathway

Zhou, Xiaoyu; Zhang, Dongjie; Wang, Mengling; Zhang, Ding; Xu, Yiming

doi:10.12659/msm.918517

Cited by 14 publications

(10 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the methodology of IGF-1 supplementation is highly promising for engineering de novo vasculature in tissue regeneration [81]. Three-dimensional (3D) culture of mesenchymal stem cells with 3D titanium scaffolds enhances osteogenic differentiation and new bone formation through the IGF-1R/AKT/ mTORC1 pathway [82]. This method of osteointegration may have clinical application in the preparation of bone grafts before implantation to improve the repair of mandibular bone defects [82].…”

Section: Osteocytes Governing Bone Regenerationmentioning

confidence: 99%

Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

Cao

Helder

Bravenboer

et al. 2020

Curr Osteoporos Rep

View full text Add to dashboard Cite

Purpose of Review Bone regeneration plays an important role in contemporary clinical treatment. Bone tissue engineering should result in successful bone regeneration to restore congenital or acquired bone defects in the human skeleton. Osteocytes are thought to have a governing role in bone remodeling by regulating osteoclast and osteoblast activity, and thus bone loss and formation. In this review, we address the so far largely unknown role osteocytes may play in bone tissue regeneration. Recent Findings Osteocytes release biochemical signaling molecules involved in bone remodeling such as prostaglandins, nitric oxide, Wnts, and insulin-like growth factor-1 (IGF-1). Treatment of mesenchymal stem cells in bone tissue engineering with prostaglandins (e.g., PGE2, PGI2, PGF2α), nitric oxide, IGF-1, or Wnts (e.g., Wnt3a) improves osteogenesis. Summary This review provides an overview of the functions of osteocytes in bone tissue, their interaction with other bone cells, and their role in bone remodeling. We postulate that osteocytes may have a pivotal role in bone regeneration as well, and consequently that the bone regeneration process may be improved effectively and rapidly if osteocytes are optimally used and stimulated.

show abstract

Section: Osteocytes Governing Bone Regenerationmentioning

confidence: 99%

Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

Cao

Helder

Bravenboer

et al. 2020

Curr Osteoporos Rep

View full text Add to dashboard Cite

show abstract

“…Our previous studies demonstrated that IGF-1R has a crucial impact on the osteo/odontogenic differentiation of DPSCs and SCAPs ( Shu et al, 2016 ; Liu et al, 2018 ). Similarly, numerous studies have shown that the activation of IGF-1/IGF-1R can regulate cell senescence and osteogenesis, participate in maintaining intracellular homeostasis, and promote tooth-related tissue regeneration ( Sergi et al, 2019 ; Zhou et al, 2019 ). This study explores the role of lncRNA ANRIL/miR-7-5p/IGF-1R axis in the osteogenic differentiation of inflamed periodontal ligament stem cells for the first time.…”

Section: Introductionmentioning

confidence: 99%

Upregulating the Expression of LncRNA ANRIL Promotes Osteogenesis via the miR-7-5p/IGF-1R Axis in the Inflamed Periodontal Ligament Stem Cells

Bian

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

BackgroundLong non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) is a base length of about 3.8 kb lncRNA, which plays an important role in several biological functions including cell proliferation, migration, and senescence. This study ascertained the role of lncRNA ANRIL in the senescence and osteogenic differentiation of inflamed periodontal ligament stem cells (iPDLSCs).MethodsHealthy periodontal ligament stem cells (hPDLSCs) and iPDLSCs were isolated from healthy/inflamed periodontal ligament tissues, respectively. The proliferation abilities were determined by CCK-8, EdU assay, and flow cytometry (FCM). The methods of Western blot assay (WB), quantitative real-time polymerase chain reaction (qRT-PCR), alizarin red staining, alkaline phosphatase (ALP) staining, ALP activity detection, and immunofluorescence staining were described to determine the biological influences of lncRNA ANRIL on iPDLSCs. Senescence-associated (SA)-β-galactosidase (gal) staining, Western blot analysis, and qRT-PCR were performed to determine cell senescence. Dual-luciferase reporter assays were conducted to confirm the binding of lncRNA ANRIL and miR-7-5-p, as well as miR-7-5p and insulin-like growth factor receptor (IGF-1R).ResultsHPDLSCs and iPDLSCs were isolated and cultured successfully. LncRNA ANRIL and IGF-1R were declined, while miR-7-5p was upregulated in iPDLSCs compared with hPDLSCs. Overexpression of ANRIL enhanced the osteogenic protein expressions of OSX, RUNX2, ALP, and knocked down the aging protein expressions of p16, p21, p53. LncRNA ANRIL could promote the committed differentiation of iPDLSCs by sponging miR-7-5p. Upregulating miR-7-5p inhibited the osteogenic differentiation of iPDLSCs. Further analysis identified IGF-1R as a direct target of miR-7-5p. The direct binding of lncRNA ANRIL and miR-7-5p, miR-7-5p and the 3′-UTR of IGF-1R were verified by dual-luciferase reporter assay. Besides, rescue experiments showed that knockdown of miR-7-5p reversed the inhibitory effect of lncRNA ANRIL deficiency on osteogenesis of iPDLSCs.ConclusionThis study disclosed that lncRNA ANRIL promotes osteogenic differentiation of iPDLSCs by regulating the miR-7-5p/IGF-1R axis.

show abstract

“…Their results showed that topological modification could significantly influence the porous properties to a greater extent than the material type (37). Also, Zhou et al (38) verified the osteogenic effect of 3D scaffold-based scaffold on adipose stem cells and confirmed that its mechanism was related to the IGF-1R/ protein kinase B (AKT)/Mammalian Target of Rapamycin Complex 1 (mTORC1) cell signaling pathway.…”

Section: Surface and Three-dimensional (3d) Structure Transformationmentioning

confidence: 97%

Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review

Zuo

Lin

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Owing to their excellent biocompatibility and corrosion-resistant properties, titanium (Ti) (and its alloy) are essential artificial substitute biomaterials for orthopedics. However, flaws, such as weak osteogenic induction ability and higher Young's modulus, have been observed during clinical application. As a result, short-and long-term postoperative follow-up has found that several complications have occurred.For decades, scientists have exerted efforts to compensate for these deficiencies. Different modification methods have been investigated, including changing alloy contents, surface structure transformation, threedimensional (3D) structure transformation, coating, and surface functionalization technologies. The cellsurface interaction effect and imitation of the natural 3D bone structure are the two main mechanisms of these improved methods. In recent years, significant progress has been made in materials science research methods, including thorough research of titanium alloys of different compositions, precise surface pattern control technology, controllable 3D structure construction technology, improvement of coating technologies, and novel concepts of surface functionalization. These improvements facilitate the possibility for further research in the field of bone tissue engineering. Although the underlying mechanism is still not fully understood, these studies still have some implications for clinical practice. Therefore, for the direction of further research, it is beneficial to summarize these studies according to the basal method used. This literature review aimed to classify these technologies, thereby providing beginners with a preliminary understanding of the field.

show abstract

Three-Dimensional Printed Titanium Scaffolds Enhance Osteogenic Differentiation and New Bone Formation by Cultured Adipose Tissue-Derived Stem Cells Through the IGF-1R/AKT/Mammalian Target of Rapamycin Complex 1 (mTORC1) Pathway

Cited by 14 publications

References 29 publications

Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

Is There a Governing Role of Osteocytes in Bone Tissue Regeneration?

Upregulating the Expression of LncRNA ANRIL Promotes Osteogenesis via the miR-7-5p/IGF-1R Axis in the Inflamed Periodontal Ligament Stem Cells

Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review

Contact Info

Product

Resources

About