Three-dimensional polymeric systems for cancer cell studies

Xu, Feng; Burg, Karen J. L.

doi:10.1007/s10616-007-9065-4

Cited by 56 publications

(68 citation statements)

References 53 publications

(46 reference statements)

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“…16,18,41,42 Given the fact that CSCs are responsible for metastasis and drug resistance, the expansion of CSCs in tumor cells cultured in 3D may account for this change.…”

Section: Cellular Morphology and Growth On Electrospun Pcl 3d Scaffoldsmentioning

confidence: 99%

Expansion of breast cancer stem cells with fibrous scaffolds

Feng

Duan

et al. 2013

Integr. Biol.

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Interdisciplinary approaches for molecular and cellular life sciences ISSN 1757-9694 www.rsc.org/ibiologyVolume 5 MDA-MB-231, by ALDEFLUOR assay and mammosphere formation assay. Moreover, we observed the upregulation of epithelial to mesenchymal transition and increased invasive capability in cells cultured on PCL fibrous scaffolds. These data suggest that the increase of CSC proportion in a 3D culture system may account for the enhanced malignancy. Therefore, our PCL fibrous scaffolds can potentially be used for CSCs enrichment and anti-cancer drug screening. Insight, innovation, integrationThree dimensional (3D) culture systems have been utilized in studies of cancer metastasis and anti-tumor drug screening. Accumulating evidence indicates that cancer cells in 3D culture displayed higher malignancy and invasive capability compared to their counterparts in two-dimensional culture. In the present paper, we have fabricated a 3D polycaprolactone fibrous scaffold using an electrospinning process to evaluate how CSCs proportion in breast cancer cell lines responds to this 3D culture. Functional assay and gene profiling together indicate that culture on fibrous scaffolds increased CSCs and induced epithelial-mesenchymal-transition. This work provides a better understanding of how a 3D culture condition affects cancer cells behavior as a mixture of CSCs and non-CSCs. Thus, this knowledge potentially enlightens the design of novel biomaterials for the enrichment of CSCs, and eventually for drug screening targeting CSCs.

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“…16,18,41,42 Given the fact that CSCs are responsible for metastasis and drug resistance, the expansion of CSCs in tumor cells cultured in 3D may account for this change.…”

Section: Cellular Morphology and Growth On Electrospun Pcl 3d Scaffoldsmentioning

confidence: 99%

Expansion of breast cancer stem cells with fibrous scaffolds

Feng

Duan

et al. 2013

Integr. Biol.

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“…[20][21][22][23] Cancer cells grown in three-dimensional (3D) cultures in a polymeric ECM closely mimic the biology of tumor development in vivo and numerous studies indicate that 3D cultures are superior to traditional 2D monolayer cultures for studies of key cellular behaviors such as differentiation, proliferation, invasion, and apoptosis. [23][24][25][26][27][28][29] Cancer cells grown in 3D culture are more resistant to chemotherapeutic agents and radiation than cells in 2D culture and 3D tumor cell cultures are useful for preclinical evaluation of the cytotoxic effect of anticancer agents. 25,27,30 It is well established that multiple cell types within individual tumors have differential sensitivities to drugs and radiation both in vivo and in 3D cultures.…”

Section: Introductionmentioning

confidence: 99%

“…[23][24][25][26][27][28][29] Cancer cells grown in 3D culture are more resistant to chemotherapeutic agents and radiation than cells in 2D culture and 3D tumor cell cultures are useful for preclinical evaluation of the cytotoxic effect of anticancer agents. 25,27,30 It is well established that multiple cell types within individual tumors have differential sensitivities to drugs and radiation both in vivo and in 3D cultures.…”

Section: Introductionmentioning

confidence: 99%

Identification of virus resistant tumor cell subpopulations in three-dimensional uveal melanoma cultures

et al. 2009

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To better understand melanoma resistance to herpes simplex virus type 1 (HSV-1)-mediated oncolysis, traditional two-dimensional (2D) cultures and extracellular matrix (ECM) containing three-dimensional (3D) cultures of OCM1 and C918 uveal melanoma cells were infected with an HSV-1 strain that expresses the green fluorescent protein (GFP) marker during replication. Although 2D cultures were completely destroyed within a few days of HSV-1 inoculation, viable GFP-negative tumor cells remained detectable in 3D cultures for several weeks. Tumor cells with increased resistance to HSV-1 included cells that formed vasculogenic mimicry patterns and multicellular spheroids and cells that invaded Matrigel individually. Mechanisms of tumor resistance against HSV-1 in the 3D environment included impaired virus spread in the ECM and ECM-mediated inhibition of viral replication after viral entry into tumor cells. Observations also suggested that HSV-1 established quiescent infection in some tumor cells present in multicellular spheroids and that this could revert to productive viral infection when the tumor growth pattern changed. These findings indicate that 3D tumor cell cultures can be used to identify distinct tumor cell populations with increased resistance to HSV-1 and to explore mechanisms of ECM-mediated tumor resistance to oncolytic virotherapy.

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“…Cancer cells grown in 3D cultures in a polymeric ECM closely mimic the biology of tumor development in vivo and numerous studies indicate that 3D cultures are superior to traditional 2D monolayer cultures for studies of key cellular behaviors like differentiation, proliferation, invasion and apoptosis [6][7][8]. Cancer cells grown in 3D culture are more resistant to chemotherapeutic agents, radiation and oncolytic virotherapy than cells in 2D culture and 3D tumor cell cultures are useful for preclinical evaluation of the cytotoxic effect of anticancer agents [6,[9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Cancer cells grown in 3D culture are more resistant to chemotherapeutic agents, radiation and oncolytic virotherapy than cells in 2D culture and 3D tumor cell cultures are useful for preclinical evaluation of the cytotoxic effect of anticancer agents [6,[9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

An Emerging Role of Biorepositories In Personalized Medicine: Isolation and Processing of Primary Tumor Cells For the Establishment of 3d Tumor Cultures From Solid Tumor Specimens

Valyi‐Nagy¹

2016

Ely J Can Res

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The introduction of three-dimensional (3D) tumor cultures has revolutionized anticancer drug research as these cultures allow for the study of drug resistance mechanisms that cannot be explored in traditional two dimensional (2D) monolayer cultures. Discoveries in the 3D tumor culture field suggest that individualized drug sensitivity testing of solid tumor specimens through the establishment and use of 3D tumor cell cultures following tissue collection will become a routine service offered by modern tissue repositories as they expand from their traditional research role to active participation in personalized medicine. Unfortunately, most information related to 3D tumor cultures comes from studies using established tumor cell lines rather than primary tumor cultures. However, accumulation of genetic aberrations in cancer cell lines occurs with increasing number of passages severely limiting their usefulness for personalized medicine. There is only very limited information available concerning technologies and standard operating procedures for the efficient and routine isolation and processing of primary tumor cells for the establishment of 3D tumor cultures from solid tumor specimens. The purpose of this work was to review experimental data from the literature that may provide relevant information concerning the isolation and processing of primary tumor cells for the establishment of 3D tumor cultures. Information reviewed here may help biorepositories in the development and standardization of technologies and standard operating procedures related to the use of 3D tumor cultures.

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Three-dimensional polymeric systems for cancer cell studies

Cited by 56 publications

References 53 publications

Expansion of breast cancer stem cells with fibrous scaffolds

Expansion of breast cancer stem cells with fibrous scaffolds

Identification of virus resistant tumor cell subpopulations in three-dimensional uveal melanoma cultures

An Emerging Role of Biorepositories In Personalized Medicine: Isolation and Processing of Primary Tumor Cells For the Establishment of 3d Tumor Cultures From Solid Tumor Specimens

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