Three-dimensional organization of a transcellular tubulocisternal endoplasmic reticulum in epithelial cells of Reissner's membrane in the guinea-pig

Qvortrup, Klaus; Rostgaard, J.

doi:10.1007/bf00318670

Cited by 22 publications

(6 citation statements)

References 24 publications

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“…This relationship prompts the consideration that vesicles budding from the Golgi cisternae fuse to generate the tubular reticulum. Although a connection between the Golgi apparatus and genesis of tubular reticulum has not been entertained previously, retrospective viewing of micrographs of sweat gland clear cells (Baron et al, 1984) and Reissner's membrane cells (Qvortrup and Rostgaard, 1990) reveals close proximity of Golgi complexes to tubulocisternal reticulum in these as in Deiters cells. An attractive aspect of this hypothesis, aside from its relevance to genesis of the reticulum, pertains to its potential for explaining the enigmatic occurrence of abundant, rather characteristic Golgi complexes in cells, like Deiters cells and sweat gland clear cells, that are not generating secretory granules or lysosomes.…”

Section: ) and Perhaps Bear Some Relationship To A Glial Type Of mentioning

confidence: 99%

“…This tubular reticulum has been implicated in transcellular ion transport also in Reissner's epithelium (Qvortrup and Rostgaard, 1990) and in transport of K + upgrade across the marginal cells of the stria vascularis (Forge, 1982). Lying close to apical plasmalemma, the tubular reticulum in Deiters cells appears situated to facilitate K' resorption from Nuels space and serve as an ion reservoir or mediate ion diffusion down a gradient toward scala tympani.…”

Section: ) and Perhaps Bear Some Relationship To A Glial Type Of mentioning

confidence: 99%

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Cytologic structures unique to deiters cells of the cochlea

Spicer

Schulte

1993

Anat. Rec.

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Deiters cells in the gerbil cochlea disclosed unusual ultrastructural features. A sharp transition zone separated the cell body underlying outer hair cells into an upper compartment with numerous organelles and a lower part devoid of structures other than the microtubule stalk. Deiters cells exhibited a unique structure, the rosette complex, which consisted of a core of densely fibrillar trabeculae, enclosed in a filamentous meshwork and surrounded by tubulocisternal endoplasmic reticulum. The dense trabeculae radiated in columns downward from an apical translucent area toward a lucent zone around the nucleus. They also spread to the medial plasmalemma enveloping nerves and upward into the base of the phalanx. Frequent, small Golgi complexes bordered the tubular reticulum. The distinctive mitochondria of Deiters cells frequently paralleled the plasmalemma, revealed an elongated, often arched profile, and contained sparse, longitudinally aligned cristae. The stalk, composed of characteristic microtubule bundles resembling those in pillar cells, ascended from basal to apical plasmalemma of the cell body and into the phalanx and reticular lamina as previously described. The stalk appeared also to ramify into smaller microtubule bundles in apical cytosol penetrating the rosette complex. Nuclei in Deiters cells differed from those in hair cells in their location high in the cell and in showing chromatin dispersion indicative of more active protein synthesis.

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Section: ) and Perhaps Bear Some Relationship To A Glial Type Of mentioning

confidence: 99%

Section: ) and Perhaps Bear Some Relationship To A Glial Type Of mentioning

confidence: 99%

Cytologic structures unique to deiters cells of the cochlea

Spicer

Schulte

1993

Anat. Rec.

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“…Cells enriched in this organelle specialize to a large extent in the transepithelial movement of fluid and ions. Sites known to express TCER include epithelium of the intestine (Møllgå rd and Rostgaard, 1981), human eccrine sweat gland (Baron et al, 1984), liver (Møller et al, 1983), proximal renal tubule, gall bladder, and choroid plexus (Møllgå rd and Rostgaard, 1978Rostgaard, , 1981Rostgaard and Møller, 1980;Bergeron and Thiéry, 1981) as well as Reissner's membrane (Qvortrup and Rostgaard, 1990) and the stria vascularis (Forge, 1982) in the cochlea.…”

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confidence: 99%

“…Although it has been shown that strial marginal cells and Reissner's membrane epithelial cells express TCER (Forge, 1982;Qvortrup and Rostgaard, 1990), whether other cochlear cells functioning in ion transport are endowed with CR is not known. The present inquiry addressed this question by examining outer hair cells (OHCs) as part of a general survey of the cochlea for the organelle.…”

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confidence: 99%

Cytologic evidence for mechanisms of K+ transport and genesis of Hensen bodies and subsurface cisternae in outer hair cells

1998

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Abstract:A system commonly termed the tubulocisternal endoplasmic reticulum (TCER), but designated here the canalicular reticulum (CR), occurs selectively in ion-transporting epithelia, in which it is interpreted as facilitating the transcellular diffusion of ions. Mechanoelectrical transduction in the cochlear outer hair cells (OHCs) depends on the apical influx and the subsequent basolateral efflux of K ϩ . Cytologic structures that possibly mediate K ϩ transport in gerbil OHCs were investigated here.Methods: Cochleas were fixed primarily with glutaraldehyde and secondarily with reagents for demonstrating TCER or were fixed with a ferrocyanideosmium tetroxide solution to preserve intracellular membranes. The distribution of membranous structures retained with these techniques was examined by using electron microscopy.Results: Secondary fixation with osmium tetroxide-ferrocyanide permitted ultrastructural demonstration in OHCs of increased numbers of Hensen bodies and newly detected membranous systems, including CR, linear cisternae, small clusters of cytosolic vesicles and complexes of canaliculi, segmented cisternae, and mitochondria. CR filling an apical stratum beside and below the cuticular plate and contacting laterally the uppermost subsurface cisternae (SSC) was situated to sequester and transport the apical K ϩ influx that attends the acoustically generated receptor potential and the silent current. The close association of CR with numerous, highly developed Golgi bodies exclusively in the apex of the cell suggested genesis of CR from Golgi cisternae. Nonbranching, linear cisternae occupied a lower cell stratum and spread from CR laterally to a more inferior region of the SSC. Small clusters of vesicles in the central cytosol resembled Hensen bodies in their envelopment by branching canaliculi and segmented cisternae in close association with mitochondria. Viewing the vesicles in Hensen bodies and the small clusters as functioning like most other cytoplasmic vesicles in transport of cell membrane permitted the interpretation that these vesicles move nascent membrane from the canalicular-mitochondrial complex to the SSC. Other small clusters of vesicles contacted the innermost layer of the SSC, often at cisterna-depleted foci in which the vesicles appeared to either replenish the SSC or arise in the course of its turnover. Proximity of multivesicular bodies and lysosomes to small vesicle clusters in foci of depleted SSC implicated the

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“…, 1978Rostgaard and Møllgård, 1980;Bergeron and Thiéry, 1981), and human eccrine sweat gland (Baron et al, 1984). In the cochlea, strial marginal cells (Forge, 1982) and Reissner's epithelium (Qvortrup and Rostgaard, 1990) contain TCER. The CR shown in cochlear fibrocytes and sulcus cells resembles TCER and presumably functions similarly in mediating ion and fluid movement.…”

Section: Discussionmentioning

confidence: 99%

Golgi–canalicular reticulum system in ion transporting fibrocytes and outer sulcus epithelium of gerbil cochlea

Spicer

Schulte

1997

Anat. Rec.

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Background: Five types of highly specialized fibrocytes have been identified in the spiral ligament of the gerbil cochlea. Type I, II, and IV fibrocytes function in cycling back to the stria vascularis K ؉ effluxed from outer hair cells and nerves during auditory transduction. Thus, evidence exists for a transcellular path of K ؉ movement from outer sulcus cells through fibrocytes to the strial interstitial space, but a mechanism for facilitating such ion flow within the cells has not been elucidated.Methods: The spiral ligament of glutaraldehyde-osmium tetroxide-fixed and Epon-embedded gerbil cochlea was examined by transmission electron microscopy.Results: Ultrastructural examination disclosed an extensive membrane limited reticulum in the cytoplasm of type I, II, IV, and V fibrocytes of the lateral wall and in outer sulcus cells and their root processes. This system resembled the tubulocisternal endoplasmic reticulum present in some ion-transporting epithelia but appeared more to constitute a network of canaliculi and is referred to as the canalicular reticulum (CR). Many typical small Golgi complexes invariably accompanied the CR in the fibrocytes and sulcus cells, as we have found to be true of other epithelia known to contain CR and function in ion transport. Numerous mitochondria populated cytosol-containing CR.Conclusions: The data support the concept of transcellular K ؉ flux in type I, II, IV, and V fibrocytes and outer sulcus cells in the cochlea and lend credence to the view of CR as functioning in the movement of ions through cells. The constant and precise association of Golgi complexes with CR in the different cell types implies a functional relationship possibly concerned with biosynthesis of CR by Golgi elements, and the abundance of mitochondria near CR indicates an energy requirement for function of the reticulum or its biosynthesis. Anat.

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Three-dimensional organization of a transcellular tubulocisternal endoplasmic reticulum in epithelial cells of Reissner's membrane in the guinea-pig

Cited by 22 publications

References 24 publications

Cytologic structures unique to deiters cells of the cochlea

Cytologic structures unique to deiters cells of the cochlea

Cytologic evidence for mechanisms of K+ transport and genesis of Hensen bodies and subsurface cisternae in outer hair cells

Golgi–canalicular reticulum system in ion transporting fibrocytes and outer sulcus epithelium of gerbil cochlea

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