Three-dimensional morphology of the human embryonic brain

Shiraishi, Naoki; Katayama, A.; Nakashima, Takashi; Yamada, Shigehito; Uwabe, Chigako; Kose, Katsumi; Takakuwa, Tetsuya

doi:10.1016/j.dib.2015.05.001

Cited by 4 publications

(2 citation statements)

References 5 publications

(7 reference statements)

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“… [1] Nakashima et al , [2] Kobayashi et al , [3–5] Shiraishi et al , [6] Shiraishi et al , [7] Taketani et al , [8] Yoshida et al, in press, [9] Takakuwa et al, , [10] Mehemed et al, , [11] Kishimoto et al, , [12,13] Toyoda et al, , [14] Kagurasho et al, , [15] Ozeki‐Sato et al, a, [16] Ozeki‐Sato et al, , [17] Osaka et al, , [18] Ueno et al, , [19] Kaigai et al, . [20] Nako et al, , [21] Ueda et al, , [22,23] Hirose et al, , 2016, [24] Hamabe et al, , [25] Kanahashi et al, , [26] Endo et al, , [27] Takakuwa et al, , [28] Shiraishi et al, .…”

Section: Methodsmentioning

confidence: 99%

3D Analysis of Human Embryos and Fetuses Using Digitized Datasets From the Kyoto Collection

Takakuwa

2018

The Anatomical Record

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Three-dimensional (3D) analysis of the human embryonic and early-fetal period has been performed using digitized datasets obtained from the Kyoto Collection, in which the digital datasets play a primary role in research. Datasets include magnetic resonance imaging (MRI) acquired with 1.5 T, 2.35 T, and 7 T magnet systems, phase-contrast X-ray computed tomography (CT), and digitized histological serial sections. Large, high-resolution datasets covering a broad range of developmental periods obtained with various methods of acquisition are key elements for the studies. The digital data have gross merits that enabled us to develop various analysis. Digital data analysis accelerated the speed of morphological observations using precise and improved methods by providing a suitable plane for a morphometric analysis from staged human embryos. Morphometric data are useful for quantitatively evaluating and demonstrating the features of development and for screening abnormal samples, which may be suggestive in the pathogenesis of congenital malformations. Morphometric data are also valuable for comparing sonographic data in a process known as "sonoembryology." The 3D coordinates of anatomical landmarks may be useful tools for analyzing the positional change of interesting landmarks and their relationships during development. Several dynamic events could be explained by differential growth using 3D coordinates. Moreover, 3D coordinates can be utilized in mathematical analysis as well as statistical analysis. The 3D analysis in our study may serve to provide accurate morphologic data, including the dynamics of embryonic structures related to developmental stages, which is required for insights into the dynamic and complex processes occurring during organogenesis. Anat Rec, 301:960-969, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Methodsmentioning

confidence: 99%

3D Analysis of Human Embryos and Fetuses Using Digitized Datasets From the Kyoto Collection

Takakuwa

2018

The Anatomical Record

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“…During prenatal development, structural changes occur in the brain and the basic elements of the definitive brain are formed. At various stages of prenatal development, a detailed topographic-histological examination of the human brain will not only help in understanding the highly ordered process of its structure, but also help to identify developmental defects that are related to genetic or environmental factors [5,8,11,15,18,22].…”

Section: Introductionmentioning

confidence: 99%

Histostructural organization of the cerebellum of human fetuses for 8-9 weeks of prenatal development

2019

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The high incidence of anomalies in the hindbrain is due to the fact that neurulation in the cranial compartment lasts the longest time. Therefore, for more detailed study of embryogenesis and understanding of mechanisms of pathogenesis, occurrence of congenital malformations, there is a need to determine the morphometric (histometric) parameters of the cerebellum at different gestational times. The purpose of the study is to determine the morphometric parameters of the cerebellum of human embryos for 8-9 weeks of prenatal development, as well as features of cytoarchitectonics of its structures, which is inherent in the given gestation period. Anatomo-histological, immunohistochemical and morphometric examination of the cerebellum of 10 human embryos was performed. Serial sections of cerebellum preparations 8-10 microns thick were stained with hematoxylin, eosin, toluidine blue and Van Gieson, and diagnostic monoclonal antibodies of “DacoCytomation” (Denmark): Vimentin, Ki-67 and Synaptophysin were used for immunohistochemical studies. The results of measurements of the total thickness of all layers, the density of neural stem cells (NSC), as well as the area of the right and left hemispheres of the cerebellum were obtained during the study. In immunohistochemical study, we determined the direction of NSC migration and cell proliferation of all layers of the cerebellum, as well as the length of radial glia fibers. In the cerebral hemispheres of embryos of 8-9 weeks there is a clear division into ventricular, intermediate, molecular and outer granular layers. The highest density of neural stem cells was observed in the outer granular layer – 151.0±4.1 cells per 0.01 mm2. The lowest cell density was observed in the molecular layer – 22.0±0.8 cells per 0.01 mm2. The most intense cell proliferation was established in the ventricular layer and the outer granular layer of the cerebellum, and the least intense in the intermediate layer. Synaptophysin expression was only slightly expressed in the ventricular layer of the cerebellum. The radial glial fibers begin from the ventricular layer and penetrate all layers of the cerebellum, ending in the outer granular layer. The average length of radial glial fibers was: short – 120.8±5.7 μm, long – 195.3±9.4 μm. The exterior granular layer is represented by spherical undifferentiated cells with an average area size of 641.1±28.9 μm2, the molecular layer – NSC with an area of 472.9±23.7 μm2, the intermediate layer – NSC with an area of 492.2±23.1 μm2, and the ventricular layer is represented by neuroblasts with an area of 436.1±21.8 μm2. Thus, it is established that there is a clear division of the cerebellum layers into the ventricular layer, which is represented by neuroblasts, the intermediate layer – NSC, the molecular layer – NSC, and the outer granular layer is represented by undifferentiated cells; the densest neural stem cells are located in the outer granular layer and less densely in the molecular layer.

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Sapiens and Cognition: The Optimal Vertical Nervous System—The Last Threshold of Self-Organized and Self-Memorizing Increasing Complexity from Gametes to Embryo

Malassé

2022

Evolutionary Biology – New Perspectives on Its Development

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Three-dimensional morphology of the human embryonic brain

Cited by 4 publications

References 5 publications

3D Analysis of Human Embryos and Fetuses Using Digitized Datasets From the Kyoto Collection

3D Analysis of Human Embryos and Fetuses Using Digitized Datasets From the Kyoto Collection

Histostructural organization of the cerebellum of human fetuses for 8-9 weeks of prenatal development

Sapiens and Cognition: The Optimal Vertical Nervous System—The Last Threshold of Self-Organized and Self-Memorizing Increasing Complexity from Gametes to Embryo

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