3D Analysis of Human Embryos and Fetuses Using Digitized Datasets From the Kyoto Collection

Abstract

Three-dimensional (3D) analysis of the human embryonic and early-fetal period has been performed using digitized datasets obtained from the Kyoto Collection, in which the digital datasets play a primary role in research. Datasets include magnetic resonance imaging (MRI) acquired with 1.5 T, 2.35 T, and 7 T magnet systems, phase-contrast X-ray computed tomography (CT), and digitized histological serial sections. Large, high-resolution datasets covering a broad range of developmental periods obtained with various methods of acquisition are key elements for the studies. The digital data have gross merits that enabled us to develop various analysis. Digital data analysis accelerated the speed of morphological observations using precise and improved methods by providing a suitable plane for a morphometric analysis from staged human embryos. Morphometric data are useful for quantitatively evaluating and demonstrating the features of development and for screening abnormal samples, which may be suggestive in the pathogenesis of congenital malformations. Morphometric data are also valuable for comparing sonographic data in a process known as "sonoembryology." The 3D coordinates of anatomical landmarks may be useful tools for analyzing the positional change of interesting landmarks and their relationships during development. Several dynamic events could be explained by differential growth using 3D coordinates. Moreover, 3D coordinates can be utilized in mathematical analysis as well as statistical analysis. The 3D analysis in our study may serve to provide accurate morphologic data, including the dynamics of embryonic structures related to developmental stages, which is required for insights into the dynamic and complex processes occurring during organogenesis. Anat Rec, 301:960-969, 2018. © 2018 Wiley Periodicals, Inc.

Critical Growth Processes for the Midfacial Morphogenesis in the Early Prenatal Period

“…Since 1961, a large number of human conceptuses have been kept in the Congenital Anomaly Research Center at Kyoto University; this is called the Kyoto Collection (Yamada et al, 2006; Takakuwa, 2018; Yamada et al, 2018a; Yamada et al, 2018b). Among them, specimens without any distinct congenital anomalies and artificial deformities of the face were selected for the present study.…”

Morphology and morphometry of the human early foetal brain: A three‐dimensional analysis

“…Further, upon revealing a thick, densely packed cellular ventricular/subventricular zone and ganglionic eminence, this method can detect the intensive proliferation of neuroepithelial stem cells. MRI data may be advantageous for precise 3D reconstruction, including volume and length measurements, for both external and internal structures (Takakuwa, 2018). Documenting morphometry during the early foetal period is important for evaluating normal growth.…”

Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis

“…We have recently reported a three‐dimensional (3‐D) analysis of human embryonic development using digital imaging data techniques such as magnetic resonance imaging (MRI) and phase contrast X‐ray computed tomography (PXCT) . Such 3‐D data are advantageous in that they acquire the anatomical landmarks with 3‐D coordinates.…”

“…11,14,15 We have recently reported a three-dimensional (3-D) analysis of human embryonic development using digital imaging data techniques such as magnetic resonance imaging (MRI) and phase contrast X-ray computed tomography (PXCT). 16 Such 3-D data are advantageous in that they acquire the anatomical landmarks with 3-D coordinates. This enables a potential understanding of the 3-D development and growth of each anatomical landmark according to the positional change of the 3-D coordinates.…”