Three Dimensional Macroporous Calcium Phosphate Scaffolds for Bone Tissue Engineering

Teixeira, Sérgio; Oliveira, Serafim M.; Ferraz, Maria Pia; Monteiro, F.J.

doi:10.4028/www.scientific.net/kem.361-363.947

Cited by 10 publications

(8 citation statements)

References 3 publications

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“…Macropores with diameter greater than 100 μm were achieved as well as a highly defined microporosity with pores ranging from 1 to 10 μm. Interconnected porosity was also achieved as observed by SEM and confirmed by other techniques such as μ‐CT 33…”

Section: Resultssupporting

confidence: 73%

“…Macropores with diameter greater than 100 lm were achieved as well as a highly defined microporosity with pores ranging from 1 to 10 lm. Interconnected porosity was also achieved as observed by SEM and confirmed by other techniques such as l-CT. 33 Analyzing the bone forming ability of cells seeded on the different scaffolds it was observed that relevant amounts of bone were formed, regardless the presence of collagen, for rMSCs. In the case of hMSCs, it was found that the HA scaffolds performed as good as the control scaffold (BCP).…”

Section: Resultsmentioning

confidence: 99%

“…1(A)] used in this study were characterized by a homogeneous macroporosity with a diameter larger than 300 lm and micropores of 1-10 lm with established interconnectivity. 33 Upon incorporation of collagen, no significant changes were observed in terms of porosity [ Fig. 1(B)].…”

Section: Scaffold Characterizationmentioning

confidence: 99%

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In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira

Fernandes

Leusink

et al. 2009

J Biomedical Materials Res

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During the last decades, different materials of both natural and synthetic origin have been developed with the aim of inducing and controlling osteogenic differentiation of mesenchymal stem cells (MSCs). In order for that to happen, it is necessary that the material to be implanted obey a series of requirements, namely: osteoconduction, biocompatibility, and biodegradability. Additionally, they must be low-priced, easy to produce, shape, and store. Hydroxyapatite (HA) is a well known ceramic with a composition similar to the mineral component of bone and is highly biocompatible and easy to obtain and/or process. On the other hand, collagen is the main structural protein present in the human body and bone. In this study, a polymer replication method was applied and a highly porous HA scaffold was produced. Collagen was later incorporated to improve the biological properties of the scaffold while resembling the bone composition. The scaffolds were characterized by means of scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive spectroscopy. In vitro and in vivo testing was performed in all scaffolds produced. The goal of this study was to evaluate the in vivo osteogenic potential of MSCs from two different species seeded on the different HA basedporous scaffolds with collagen type I. The resultsindicate that all scaffolds exhibit relevant bone formation, being more prominent in the case of the HA scaffolds.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

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In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira

Fernandes

Leusink

et al. 2009

J Biomedical Materials Res

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“…A high porosity and adequate pore dimensions ( Table 2 and Table 6 ) are very important to allow cell migration, vascularization, as well as diffusion of nutrients [ 352 ]. Namely, scaffolds should have a network of interconnected pores where more than ~60% of the pores should have a size ranging from ~150 μm to ~400 μm and at least ~20% should be smaller than ~20 μm [ 11 , 105 , 352 , 362 , 362 , 409 , 410 , 411 , 412 , 413 , 414 , 415 , 457 , 615 , 616 , 617 , 618 , 619 , 620 , 621 ]. Scaffolds must be manufactured from materials with controlled biodegradability and/or bioresorbability, such as calcium orthophosphate bioceramics, so that new bone will eventually replace the scaffold [ 622 ].…”

Section: Calcium Orthophosphate Bioceramics In Tissue Engineeringmentioning

confidence: 99%

Calcium Orthophosphates as Bioceramics: State of the Art

Dorozhkin¹

2010

JFB

214

112

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In the late 1960s, much interest was raised in regard to biomedical applications of various ceramic materials. A little bit later, such materials were named bioceramics. This review is limited to bioceramics prepared from calcium orthophosphates only, which belong to the categories of bioactive and bioresorbable compounds. There have been a number of important advances in this field during the past 30–40 years. Namely, by structural and compositional control, it became possible to choose whether calcium orthophosphate bioceramics were biologically stable once incorporated within the skeletal structure or whether they were resorbed over time. At the turn of the millennium, a new concept of calcium orthophosphate bioceramics—which is able to promote regeneration of bones—was developed. Presently, calcium orthophosphate bioceramics are available in the form of particulates, blocks, cements, coatings, customized designs for specific applications and as injectable composites in a polymer carrier. Current biomedical applications include artificial replacements for hips, knees, teeth, tendons and ligaments, as well as repair for periodontal disease, maxillofacial reconstruction, augmentation and stabilization of the jawbone, spinal fusion and bone fillers after tumor surgery. Exploratory studies demonstrate potential applications of calcium orthophosphate bioceramics as scaffolds, drug delivery systems, as well as carriers of growth factors, bioactive peptides and/or various types of cells for tissue engineering purposes.

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“…In addition, the presence of macroporosity induces osteoinduction. 26,27 Furthermore, the presence of micro [ Fig. 2(E)] and nanoporosity [ Fig.…”

Section: Discussionmentioning

confidence: 99%

Heparinized nanohydroxyapatite/collagen granules for controlled release of vancomycin

Coelho

Sousa

Monteiro

2015

J. Biomed. Mater. Res.

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The purpose of this study was to develop a bone substitute material capable of preventing or treating osteomyelitis through a sustainable release of vancomycin and simultaneously inducing bone regeneration. Porous heparinized nanohydroxyapatite (nanoHA)/collagen granules were characterized using scanning electron microscopy, microcomputed tomography and attenuated total reflectance Fourier transform infrared spectroscopy. After vancomycin adsorption onto the granules, its releasing profile was studied by UV molecular absorption spectroscopy. The heparinized granules presented a more sustainable release over time, in comparison with nonheparinized nanoHA and nanoHA/collagen granules. Vancomycin was released for 360 h and proved to be bioactive until 216 h. Staphylococcus aureus adhesion was higher on granules containing collagen, guiding the bacteria to the material with antibiotic, improving their eradication. Moreover, cytotoxicity of the released vancomycin was assessed using osteoblast cultures, and after 14 days of culture in the presence of vancomycin, cells were able to remain viable, increasing their metabolic activity and colonizing the granules, as observed by scanning electron microscopy and confocal laser scanning microscopy. These findings suggest that heparinized nanoHA/collagen granules are a promising material to improve the treatment of osteomyelitis, as they are capable of releasing vancomycin, eliminating the bacteria, and presented morphological and chemical characteristics to induce bone regeneration.

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Three Dimensional Macroporous Calcium Phosphate Scaffolds for Bone Tissue Engineering

Cited by 10 publications

References 3 publications

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Calcium Orthophosphates as Bioceramics: State of the Art

Heparinized nanohydroxyapatite/collagen granules for controlled release of vancomycin

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