<i>In vivo</i> evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Teixeira, Sérgio; Fernandes, Hugo; Leusink, Anouk; Blitterswijk, Clemens A. van; Ferraz, Maria Pia; Monteiro, Fernando J.; Boer, Jan de

doi:10.1002/jbm.a.32532

Cited by 60 publications

(38 citation statements)

References 45 publications

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“…The possibility of culturing cell seeded scaffolds for at least 5 days provides an advantage for tissue engineering purposes. In bone tissue engineering, for instance, the in vivo bone forming potential of cells is often assessed by seeding them on scaffolds, followed by in vitro culture for 7 days prior to implantation in an animal model [26]. Researchers frequently use scaffolds with poorly defined architectures, which compromises the quality of the tissue engineered grafts and the success of in vivo bone formation.…”

Section: Static Culturing Of Imsc On the Different Scaffoldsmentioning

confidence: 99%

Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing

et al. 2010

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The advance of rapid prototyping techniques has significantly improved control over the pore network architecture of tissue engineering scaffolds. In this work, we have assessed the influence of scaffold pore architecture on cell seeding and static culturing, by comparing a computer designed gyroid architecture fabricated by stereolithography with a random pore architecture resulting from salt leaching. The scaffold types showed comparable porosity and pore size values, but the gyroid type showed a more than 10-fold higher permeability due to the absence of size-limiting pore interconnections. The higher permeability significantly improved the wetting properties of the hydrophobic scaffolds and increased the settling speed of cells upon static seeding of immortalised mesenchymal stem cells. After dynamic seeding followed by 5 days of static culture gyroid scaffolds showed large cell populations in the centre of the scaffold, while salt-leached scaffolds were covered with a cell sheet on the outside and no cells were found in the scaffold centre. It was shown that interconnectivity of the pores and permeability of the scaffold prolonged the time of static culture before overgrowth of cells at the scaffold periphery occurred. Furthermore, novel scaffold designs are proposed to further improve the transport of oxygen and nutrients throughout the scaffolds and to create tissue engineering grafts with a designed, pre-fabricated vasculature.

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Section: Static Culturing Of Imsc On the Different Scaffoldsmentioning

confidence: 99%

Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing

et al. 2010

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“…Despite the advantages, hydroxyapatite use has been limited because of its brittle nature, low tensile strength, and high infection rates (Table 1). Hydroxyapatite has demonstrated osteointegration in animal models; 19,21,26 however, there is not much evidence for osteointegration for humans in vivo. 9 Frassanito et al demonstrated that hydroxyapatite may break down into many fragments over time in vivo, and they also demonstrated extremely limited osteointegration that lacked lamellar organization.…”

Section: Hydroxyapatitementioning

confidence: 99%

Materials used in cranioplasty: a history and analysis

Shah

Jung

Skirboll

2014

FOC

313

305

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Cranioplasty, one of the oldest surgical procedures used to repair cranial defects, has undergone many revolutions over time to find the ideal material to improve patient prognosis. Cranioplasty offers cosmetic and protective benefits for patients with cranial defects. The first primitive cranioplasty procedures date back to 7000 bc and used metal and gourds to repair cranial defects. Cranioplasty was first documented by Fallopius who described repair using gold plates; the first bone graft was documented by van Meekeren. The first significant improvement for this procedure began with experimentation involving bone grafts in the late 19th century as a more natural approach for repairing cranial defects. The next impetus for advancement came because of wartime injuries incurred during World Wars I and II and involved experimentation with synthetic materials to counter the common complications associated with bone grafts. Methyl methacrylate, hydroxyapatite, ceramics, and polyetheretherketone implants among other materials have since been researched and used. Research now has shifted toward molecular biology to improve the ability of the patient to regenerate bone using bone growth factors. This paper reviews the evolution of materials used over time in addition to the various advantages and pitfalls associated with each change. It is important for neurosurgeons to be mindful of how these techniques have evolved in order to gain a better understanding of this procedure and how it has been adapted.

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“…It though has disadvantages like, low tensile strength, high infection, brittleness, disintegration with time and unpredictable response when exposed to blood or cerebrospinal fl uid. 14,23,33 Patients with hydroxyapatite must stay away from trauma till full bone is formed.…”

Section: Hydroxyapatitementioning

confidence: 99%

Review of the History of Materials Used With Experience with Bone Cement Cranioplasty

Roka¹

2017

Nep J Neurosci

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Cranioplasty is the surgical repair of cranial defect or deficiency of the skull with a aim for functional and cosmetic improvement. Inorganic and organic materials have both been used for cranioplasty with the ideal cranioplasty material is yet to be made or discovered with present focus of research on molecular biology. This article does a brief review of the history of materials used and discusses the results of the use of bone cement cranioplasty (BCC) in this centre. A total of 61 cases were included in the study with the majority being males (44 cases). Road traffic accident was the most common initial cause of injury (45 cases), followed by fall (10 cases) and physical assault in the rest. On admission and based on the GCS based head injury classification the majority were in the severe head injury type (GCS<8) in 44 cases followed by moderate head injury in the rest. The majority of the decompressive craniectomy was done on the left side (32 cases) followed by right (22 cases) and bilateral in 7 cases (including single fl apbifrontal in 5 cases). In our centre as for other centres in Nepal and developing countries, bone cement remains the best, safest, cheapest choice along with autologous graft for cranioplasty.Nepal Journal of Neuroscience, Volume 14, Number 1, 2017, Page: 7-13

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In vivo evaluation of highly macroporous ceramic scaffolds for bone tissue engineering

Cited by 60 publications

References 45 publications

Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing

Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing

Materials used in cranioplasty: a history and analysis

Review of the History of Materials Used With Experience with Bone Cement Cranioplasty

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