Three-dimensional hierarchical plasmonic nano-architecture based label-free surface-enhanced Raman spectroscopy detection of urinary exosomal miRNA for clinical diagnosis of prostate cancer

Recent Advances in Exosomal miRNA Biosensing for Liquid Biopsy

“…Kim et al combined 3D plasmonic nanostructures with SERS strategy for designing a quantitative label-free biosensor to detect urinary exo-miRNA [ 77 ]. As shown in Figure 5 A, they coupled self-assembled DNA probes to three-dimensional gold nanostructured particles called SAP-AuNPs.…”

Recent progress in surface-enhanced Raman spectroscopy-based biosensors for the detection of extracellular vesicles

“…In summary, this method was successfully applied by indirectly analyzing EV miRNA in vivo , which offers the potential to quickly, accurately, and conveniently diagnose diseases using indirect SERS detection. A miRNA sensing platform was built by Kim et al 45 for the non-invasive diagnosis of prostate cancer through detecting miRNA from urine. This platform captures EV miRNA using an Au-nanopillar-exploiting DNA probe, in which SERS signals are detected by self-assembly of the DNA probe and AuNPs (Fig.…”

A Novel ECL Sensing System for Ultrahigh Sensitivity miRNA-21 Detection Based on Catalytic Hairpin Assembly Cascade Nonmetallic SPR Effect

“…MicroRNAs (miRNAs), a class of small and nonprotein-coding RNA molecules containing about 19–24 nucleotides, play an extremely important role in the regulation of various biological processes and gene expression. − miRNA-21 is widely presented in lung cancer, breast cancer, cervical cancer, colorectal cancer, prostate cancer, and other tumor cells, and it shows high concentrations in these cells. , Therefore, accurate and sensitive detection of miRNA-21 is of great value for the early diagnosis and treatment of tumors. Up to now, numerous analytical methods have been developed to monitor miRNA-21, such as Northern blotting, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), colorimetrics, , surface-enhanced Raman, , and electrochemical methods. , For example, a microfluidic surface-enhanced Raman scattering (SERS) sensor was designed for microRNA detection by a nucleic acid-tyramine cascade amplification . A photoelectrochemical biosensor was proposed for miRNA-21 based on a b-TiO 2 /CdS:Eu/Ti 3 C 2 heterojunction .…”

“…5,6 Therefore, accurate and sensitive detection of miRNA-21 is of great value for the early diagnosis and treatment of tumors. Up to now, numerous analytical methods have been developed to monitor miRNA-21, such as Northern blotting, 7 quantitative reverse transcription-polymerase chain reaction (qRT-PCR), 8 colorimetrics, 9,10 surface-enhanced Raman, 11,12 and electrochemical methods. 13,14 For example, a microfluidic surface-enhanced Raman scattering (SERS) sensor was designed for microRNA detection by a nucleic acid-tyramine cascade amplification.…”