Three-dimensional electrospun nanofibrous scaffolds displaying bone morphogenetic protein-2-derived peptides for the promotion of osteogenic differentiation of stem cells and bone regeneration

Ye, Kaiqiang; Liu, Dinghua; Kuang, Haizhu; Cai, Jiangyu; Chen, Wei-Ming; Sun, Binbin; Xia, Lunguo; Fang, Bing; Morsi, Yos S.; Mo, Xiumei

doi:10.1016/j.jcis.2018.09.071

Cited by 114 publications

(118 citation statements)

References 48 publications

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“…Recombinant human bone morphogenetic protein 2 (rhBMP‐2), in human amnion MSCs, with nano‐HA/Coll/poly ( l ‐lactide), provides a potential approaches in tissue engineering for periodontal bone regeneration . Furthermore, in vitro and in vivo results using a rat cranial bone defect model demonstrated that the 3D nanofibrous scaffolds incorporated with nano‐hydroxylapatite (nHA) and BMP‐2 peptides exhibited favorable biocompatibility and osteoinductivity . In addition, the DEG analysis performed by RT 2 Profiler PCR array allowed us to detect early epigenetic modifications induced in hASCs by scaffold.…”

Section: Discussionmentioning

confidence: 99%

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Mazzoni

D’Agostino

Iaquinta

et al. 2019

Stem Cells Translational Medicine

100

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Tissue engineering‐based bone graft is an emerging viable treatment modality to repair and regenerate tissues damaged as a result of diseases or injuries. The structure and composition of scaffolds should modulate the classical osteogenic pathways in human stem cells. The osteoinductivity properties of the hydroxylapatite‐collagen hybrid scaffold named Coll/Pro Osteon 200 were investigated in an in vitro model of human adipose mesenchymal stem cells (hASCs), whereas the clinical evaluation was carried out in maxillofacial patients. Differentially expressed genes (DEGs) induced by the scaffold were analyzed using the Osteogenesis RT2 PCR Array. The osteoinductivity potential of the scaffold was also investigated by studying the alkaline phosphatase (ALP) activity, matrix mineralization, osteocalcin (OCN), and CLEC3B expression protein. Fifty patients who underwent zygomatic augmentation and bimaxillary osteotomy were evaluated clinically, radiologically, and histologically during a 3‐year follow‐up. Among DEGs, osteogenesis‐related genes, including BMP1/2, ALP, BGLAP, SP7, RUNX2, SPP1, and EGFR, which play important roles in osteogenesis, were found to be upregulated. The genes to cartilage condensation SOX9, BMPR1B, and osteoclast cells TNFSF11 were detected upregulated at every time point of the investigation. This scaffold has a high osteoinductivity revealed by the matrix mineralization, ALP activity, OCN, and CLEC3B expression proteins. Clinical evaluation evidences that the biomaterial promotes bone regrowth. Histological results of biopsy specimens from patients showed prominent ossification. Experimental data using the Coll/Pro Osteon 200 indicate that clinical evaluation of bone regrowth in patients, after scaffold implantation, was supported by DEGs implicated in skeletal development as shown in “in vitro” experiments with hASCs.

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Section: Discussionmentioning

confidence: 99%

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Mazzoni

D’Agostino

Iaquinta

et al. 2019

Stem Cells Translational Medicine

100

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“…Since the fibrils of the extra cellular matrix of bone tissue are also randomly organized and have nanometer-scale diameters, the electrospun fibers applied in this study are similar as in size to what cells may encounter with it in native tissue. For instance, Ye et al [24] synthesized three-dimensional nanofibrous scaffolds via electrospining method. These electrospuned scaffolds have both nanofibrous morphologies and interconnected pores which were fabricated for the promotion of osteogenic differentiation of stem cells and bone regeneration [2,23,24].…”

Section: Discussionmentioning

confidence: 99%

The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

Mirakabad¹,

Hosseinzadeh²,

Abbaszadeh³

et al. 2019

Nano BioMed ENG

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Nowadays, bone repair by means of stem cells potential is considered as a new approach in regenerative medicine. Adipose-derived mesenchymal stem cells (AD-MSCs) have been investigated as a plentiful cell source with the ability of osteogenic differentiation which can play an important role in bone tissue engineering applications. Discovering proper elements in combination of scaffolds structure to stimulate osteogenesis in adipose-derived stem cells is one of the major concerns in this issue. Porous polymeric scaffolds such as polyacrylonitrile (PAN) and susceptible nanoparticles have attracted a lot of attention recently due to biodegradability and differentiation potential respectively. In the present study, clay-PAN nanocomposite (CPN) and graphene-PAN scaffold have been electrospuned separately and evaluated from the point of the osteogenic potential in AD-MSCs. The objective of this study was to determine the effect of clay and graphene nanoparticles with PAN nanofibers on the fate of viability and osteogenesis of AD-MSCs. First, isolated mesenchymal cells were characterized by flow cytometry. After cell culture on the surface of scaffolds MTT assay, scanning electron microscope (SEM) and DAPI staining were done. The scaffolds were characterized and osteogenic differentiation potential of AD-MSCs has been investigated. The results have indicated that alkaline phosphatase (ALP) activity, calcium content and collagen expression of cells which cultured on clay-PAN nanofibers were higher than cells which cultured on graphene-PAN scaffold. Taken together, these results suggest that porous nanofiber clay-PAN scaffold can enhance the osteogenic differentiation of AD-MSCs, and can be used as a new biodegradable scaffold for bone tissue engineering applications.

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“…Electrospun hybrid scaffolds are effective drug delivery platforms that can both promote tissue regeneration and be used for disease prevention because of their high flexibility in loading drugs . Drugs can be incorporated into electrospun scaffolds in a variety of methods, such as blending, physical adsorption, coating, and embedded loading . For blending, the release rate mainly depends on the properties of polymers and drugs in the physiological environments.…”

Section: Polymer Fiber Scaffolds Promote Bone Cartilage and Osteochmentioning

confidence: 99%

“…In terms of the physical adsorption, such as electrostatic adsorption and hydrogen bonding, drugs usually exhibit a relatively fast release rate due to the weak interaction between drugs and the surface of scaffolds. About the coaxial electrospinning, bioactive substances like drugs could be incorporated into the core structure of nanofibers by dissolving in the core solution, then released from the nanofibers in a sustained manner in vivo 107a,108…”

Section: Polymer Fiber Scaffolds Promote Bone Cartilage and Osteochmentioning

confidence: 99%

Polymer Fiber Scaffolds for Bone and Cartilage Tissue Engineering

Zhang

Liu

Zeng

et al. 2019

Adv Funct Materials

218

154

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Successful regeneration of weight-bearing bone defects and criticalsized cartilage defects remains a major challenge in clinical orthopedics. In the past decades, biodegradable polymer materials with biomimetic chemical and physical properties have been rapidly developed as ideal candidates for bone and cartilage tissue engineering scaffolds. Due to their unique advantages over other materials of high specific-surface areas, suitable mechanical strength, and tailorable characteristics, scaffolds made of polymer fibers have been increasingly used for the repair of bone and cartilage defects. This Review summarizes the preparation and compositions of polymer fibers, as well as their characteristics. More importantly, the applications of polymer fiber scaffolds with well-designed structures or unique properties in bone, cartilage, and osteochondral tissue engineering have been comprehensively highlighted. On the whole, such a comprehensive summary affords constructive suggestions for the development of polymer fiber scaffolds in bone and cartilage tissue engineering.

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Three-dimensional electrospun nanofibrous scaffolds displaying bone morphogenetic protein-2-derived peptides for the promotion of osteogenic differentiation of stem cells and bone regeneration

Cited by 114 publications

References 48 publications

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

Hydroxylapatite-collagen hybrid scaffold induces human adipose-derived mesenchymal stem cells to osteogenic differentiation in vitro and bone regrowth in patients

The Comparison between the Osteogenic Differentiation Potential of Clay-Polyacrylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

Polymer Fiber Scaffolds for Bone and Cartilage Tissue Engineering

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