Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells

Shin, Jisoo; Choi, Eun Jung; Cho, Jung Ho; Cho, Ann Na; Jin, Yoonhee; Yang, Kisuk; Song, Changsik; Cho, Seung Woo

doi:10.1021/acs.biomac.7b00568

Cited by 153 publications

(129 citation statements)

References 75 publications

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“…First, HAC conjugates were synthesized via a chemical reaction using 1‐ethyl‐3‐(3‐(dimethylamino)propyl)‐carbodiimide hydrochloride (EDC) and N ‐hydroxysuccinimide (NHS) as carboxyl activating agents (Figure S1, Supporting Information). The successful modification of catechol was confirmed by 1 H nuclear magnetic resonance ( 1 H NMR), which demonstrated that the degree of catechol was ≈34.2% (Figure S2, Supporting Information). Meanwhile, the ultraviolet–visible (UV–vis) spectrum of HAC showed a new peak at 280 nm (Figure S3, Supporting Information), which is attributed to the absorption of benzene ring in dopamine .…”

mentioning

confidence: 94%

Mussel‐Inspired Flexible, Wearable, and Self‐Adhesive Conductive Hydrogels for Strain Sensors

Bei

Huang

et al. 2019

Macromol. Rapid Commun.

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delamination of electronic conductors and substrates pose significant challenges in strain sensor applications such as detection of biological signals of human motion. [7] Furthermore, these traditional sensors are mounted on the skin by additional adhesives, mechanical clamps, or straps to achieve long-term practical application. [8] Currently, researchers have focused on identifying new promising candidate materials to overcome these defects.Hydrogels are three-dimensional (3D) polymeric networks with good stretchability, viscoelasticity, and swelling properties. [9] Due to their outstanding performance, many stretchable hydrogels have been increasingly applied to the field of wearable strain sensors. [10][11][12][13][14] For instance, Li et al. [15] developed a dual physically crosslinked polymer hydrogel with hydrogen bonding and ionic coordination. As a strain sensor, it has high sensitivity to pressure and deformation and can detect the human body movement. However, this hydrogel requires additional double-sided tape to be attached to the skin due to the lack of self-adhesion, which limits its application in wearable devices. Thus, an extended function (e.g., self-adhesive performance) is promising for motion detection and allows easy skin adhesion. Currently, the development of a hydrogel-based composite strain sensor with excellent stretchability and selfadhesive properties remains a considerable challenge. Recently, mussel-inspired adhesive hydrogels have demonstrated excellent performance and served as an inspiration for the design and fabrication of other types of self-adhesive hydrogels. Mussels can firmly adhere to all surfaces by noncovalent and covalent chemical interactions regardless of surface roughness. [16] Dopamine (DA), which has a protein-catechol group that is similar to that used in mussel adhesion, has been widely used to fabricate selfadhesive and conductive hydrogels. [17] More importantly, DA provides a new perspective for the development of wearable strain sensors with excellent self-adhesive properties. For instance, Liu et al. [18] reported a hydrogel-based self-adhesive and self-healing epidermal strain sensor by adding Polydopamine (PDA) to PVA; the developed sensor can detect minute epidermal deformations (0.1% strain) and large body movements (10-75% strain) such as throat vibration and finger bending. In addition, it can be used in the field of low-intensity strain sensing. Furthermore, Zhang et al. [19] prepared a conductive, healable, and self-adhesive hydrogel-based sensor by dynamic supramolecular cross-linkingThe latest generation of wearable devices features materials that are flexible, conductive, and stretchable, thus meeting the requirements of stability and reliability. However, the metal conductors that are currently used in various equipments cannot achieve these high performance expectations. Hence, a mussel-inspired conductive hydrogel (HAC-B-PAM) is prepared with a facile approach by employing polyacrylamide (PAM), dopamine-functionalized hyaluronic acid (HAC), ...

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mentioning

confidence: 94%

Mussel‐Inspired Flexible, Wearable, and Self‐Adhesive Conductive Hydrogels for Strain Sensors

Bei

Huang

et al. 2019

Macromol. Rapid Commun.

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“…Then we attached EBs in the new culture dish coated with Matrigel (BD Biosciences) with neural induction medium comprised of DMEM/F12 medium (11320033, Invitrogen), 1× N2 supplement (17502048, Invitrogen), 1× nonessential amino acids (11140050, Invitrogen) for 6 days. When neural rosette formation appeared in the center of the EBs, NPCs were collected for analysis [ 23 , 24 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Chromatin Interaction Changes during the iPSC-NPC Model to Facilitate the Study of Biologically Significant Genes Involved in Differentiation

Choi

Hwang

Lee

et al. 2020

Genes

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Given the difficulties of obtaining diseased cells, differentiation of neurons from patient-specific human induced pluripotent stem cells (iPSCs) with neural progenitor cells (NPCs) as intermediate precursors is of great interest. While cellular and transcriptomic changes during the differentiation process have been tracked, little attention has been given to examining spatial re-organization, which has been revealed to control gene regulation in various cells. To address the regulatory mechanism by 3D chromatin structure during neuronal differentiation, we examined the changes that take place during differentiation process using two cell types that are highly valued in the study of neurodegenerative disease - iPSCs and NPCs. In our study, we used Hi-C, a derivative of chromosome conformation capture that enables unbiased, genome-wide analysis of interaction frequencies in chromatin. We showed that while topologically associated domains remained mostly the same during differentiation, the presence of differential interacting regions in both cell types suggested that spatial organization affects gene regulation of both pluripotency maintenance and neuroectodermal differentiation. Moreover, closer analysis of promoter–promoter pairs suggested that cell fate specification is under the control of cis-regulatory elements. Our results are thus a resourceful addition in benchmarking differentiation protocols and also provide a greater appreciation of NPCs, the common precursors from which required neurons for applications in neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, schizophrenia and spinal cord injuries are utilized.

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“…Inclusion of electrospun fibers synthesized from collagen reduced stem cell survival and differentiation, while electrospun fibers of poly(ε-caprolactone-co-D,L-lactide) maintained stem cell survival and differentiation similar to HAMC alone [89]. Electroconductive HA composites have also been developed by incorporating carbon nanotube or polypyrrole into HA-based hydrogels [131]. Greater neuronal differentiation of human fetal neural stem cells and human induced pluripotent stem cell-derived neural progenitor cells in 3D culture was seen in these composite hydrogels compared to hydrogels alone [131].…”

Section: Composite Materials Systemsmentioning

confidence: 99%

“…Electroconductive HA composites have also been developed by incorporating carbon nanotube or polypyrrole into HA-based hydrogels [131]. Greater neuronal differentiation of human fetal neural stem cells and human induced pluripotent stem cell-derived neural progenitor cells in 3D culture was seen in these composite hydrogels compared to hydrogels alone [131].…”

Section: Composite Materials Systemsmentioning

confidence: 99%

Hyaluronic Acid Biomaterials for Central Nervous System Regenerative Medicine

Jensen

Holloway

Stabenfeldt

2020

Cells

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Hyaluronic acid (HA) is a primary component of the brain extracellular matrix and functions through cellular receptors to regulate cell behavior within the central nervous system (CNS). These behaviors, such as migration, proliferation, differentiation, and inflammation contribute to maintenance and homeostasis of the CNS. However, such equilibrium is disrupted following injury or disease leading to significantly altered extracellular matrix milieu and cell functions. This imbalance thereby inhibits inherent homeostatic processes that support critical tissue health and functionality in the CNS. To mitigate the damage sustained by injury/disease, HA-based tissue engineering constructs have been investigated for CNS regenerative medicine applications. HA’s effectiveness in tissue healing and regeneration is primarily attributed to its impact on cell signaling and the ease of customizing chemical and mechanical properties. This review focuses on recent findings to highlight the applications of HA-based materials in CNS regenerative medicine.

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Three-Dimensional Electroconductive Hyaluronic Acid Hydrogels Incorporated with Carbon Nanotubes and Polypyrrole by Catechol-Mediated Dispersion Enhance Neurogenesis of Human Neural Stem Cells

Cited by 153 publications

References 75 publications

Mussel‐Inspired Flexible, Wearable, and Self‐Adhesive Conductive Hydrogels for Strain Sensors

Mussel‐Inspired Flexible, Wearable, and Self‐Adhesive Conductive Hydrogels for Strain Sensors

Chromatin Interaction Changes during the iPSC-NPC Model to Facilitate the Study of Biologically Significant Genes Involved in Differentiation

Hyaluronic Acid Biomaterials for Central Nervous System Regenerative Medicine

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