Three-Dimensional Digital Confocal Raman Microscopy

Govil, Anurag; Pallister, David M.; Morris, Michael D.

doi:10.1366/0003702934048497

Cited by 48 publications

(34 citation statements)

References 16 publications

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“…Results of decomposition of confocal Raman spectral images, presenting a distribution of (b) water and (c) carbon dioxide in the successive set (from top to bottom) to layers of the sample. The numbers(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) correspond to the relative distances between the layers of the sample in prn.S . SHARONOV ET AL.…”

mentioning

confidence: 99%

Confocal three‐dimensional scanning laser Raman–SERS–fluorescence microprobe. Spectral imaging and high‐resolution applications

Sharonov

Nabiev

Chourpa

et al. 1994

J Raman Spectroscopy

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A multi-purpose instrument which allows the recording of confocal micro-Raman, micro-SERS and microfluorescence spectral images of sample areas from 5 x 5 to 150 x 150 pm with a lateral resolution of cu. 0.3 pm and an axial resolution of cu. 1 pm was developed. The instrument is a combination of two spectrometers both coupled to the same microscope, motorized sample stage, confocal entrance chamber, macro-sample chamber and CCD detector. The first spectrometer includes a double monochromator coupled with a spectrograph and exhibits the properties of a typical high-resolution Raman instrument permitting measurements of the low-wavenumber regions of the spectra. The second spectrometer includes a Notch filter and a spectrograph equipped with two interchangeable low-dispersion gratings and exhibits the properties of a high-luminosity spectrometer, suitable for low resolution, over a wide spectral range and highly sensitive micro-Raman and microfluorescence measurements. The choice of spectrometer most suitable for a particular application can be made automatically without additional prealignment of the system. The system of optical scanners operating in the confocal mode and two-dimensional CCD detection allow the accumulation of spectra from hundreds of points of the sample under the microscope simultaneously. A computercontrolled scanning sample stage and a system using a 'scanning line' of the laser beam allow fast recording of well resolved confocal spectral images (CSI) without sample degradation. Conventional images of species could be recorded with a TV-CCD camera through the microscope optics. The software supports all stages of CSI recording and allows the combined treatment of conventional and spectral images including their spatial calibration and conversion of spectral image into a conventional image, to assign point-bypoint the spectral data to the conventional image. The applicability of the instrumentation and techniques to the study of polymeric materials, industrial samples and fluid inclusions in minerals was demonstrated. Spectral images based on the micro-SERS analysis of an antitumour drug adsorbed on the hydrosol were recorded and are discussed in terms of their application to the micro-SERS imaging studies of living cells.

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mentioning

confidence: 99%

Confocal three‐dimensional scanning laser Raman–SERS–fluorescence microprobe. Spectral imaging and high‐resolution applications

Sharonov

Nabiev

Chourpa

et al. 1994

J Raman Spectroscopy

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“…Though Raman spectral signatures are much weaker than fluorescence emission spectra, it is possible to perform Raman spectral imaging at the single cell level with modern detection technologies. Hyperspectral Raman microscopy can be implemented in a variety of formats similar to those described for hyperspectral fluorescence microscopy (Christensen & Morris, 1998;Govil, et al, 1993;Morris, et al, 1996), however the most commonly utilized for visualizing endocytosis in living cells has been the confocal pointscanning method, due to its availability, high sensitivity, optical sectioning capability, and speed.…”

Section: Applications Of Raman Spectral Imaging In Endocytosismentioning

confidence: 99%

Advanced Optical Imaging of Endocytosis

Aaron¹,

Timlin²

2012

Molecular Regulation of Endocytosis

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“…Equation (1) requires some modifications for gaseous solutions and it can be done as follows. Fora binary mixture of gas A, containing the nucleus X whose shielding oŸ is of interest, and gas B as the solvent, it can be written as oŸ = a0(X) + O-AA(X)PA + Cr,,B(X)PB + .... (2) where PA and PB are the densities of A and B and a0(X ) is the shielding at the zero-density limit. The coefficients crAA(X ) and aAB(X ) contain the bulk susceptibility corrections ((erA) b and (aB)u), and the terms taking into account the intermolecular interactions during the binary collisions of A-A and A-B molecules are O' I(A_A~(X ) and al(A.m(X ).…”

Section: Nuclear Magnetic Shieldÿmentioning

confidence: 99%

Shielding and spin-spin coupling constants from gas-phase NMR

Jackowski

2003

Appl. Magn. Reson.

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Abstraet. Intermolecular interactions modify nuclear magnetic resonance (NMR) chemical shifts and spin-spin coupling constants. The intermolecular effects can be determined if the NMR parameters for an isolated molecule are known+ Gas-phase NMR spectroscopy offers such methods which allow one to measure the shielding and spin-spin coupling constants at the zero-density lirnit where the NMR parameters are free from intermolecular contributions. It is also shown that at present the multinuclear NMR spectra can easily be obtained for gaseous samples containing several micrograms of a solute compound.

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Three-Dimensional Digital Confocal Raman Microscopy

Cited by 48 publications

References 16 publications

Confocal three‐dimensional scanning laser Raman–SERS–fluorescence microprobe. Spectral imaging and high‐resolution applications

Confocal three‐dimensional scanning laser Raman–SERS–fluorescence microprobe. Spectral imaging and high‐resolution applications

Advanced Optical Imaging of Endocytosis

Shielding and spin-spin coupling constants from gas-phase NMR

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