Three-dimensional development of tensile pre-strained annulus fibrosus cells for tissue regeneration: An in-vitro study

Chuah, Yon Jin; Lee, Wu Chean; Wong, Hee Kit; Kang, Yuejun; Hee, Hwan Tak

doi:10.1016/j.yexcr.2014.09.022

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…Chitosan compatibility with glycosaminoglycan structure held superior biological properties at relatively low cost, where PEO affected fabricating process as a plasticizer [144,145]. Collagen coated silicon membrane was used as a temporary scaffold enable researchers to develop tensile pre-strained AF cells for tissue regeneration study in vitro [103]. Poly caprolactone, was widely used as tissue engineered scaffold, alone [100] or as a blend [70].…”

Section: Biomaterials Selectionmentioning

confidence: 99%

“…In some studies relation between development of the AF cells with simultaneous mechanical loading during cell culture process studied and it was shown that various bilateral tensile strain duration need to be optimized in order to lead cell-matrix interaction enhancement for IVD tissue engineering [13,48,103]. Table 3 indicates biochemical analysis related equipment or different assays usually had been used in different research activities.…”

Section: Biochemical Analysismentioning

confidence: 99%

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Tissue Engineering of the Intervertebral Disc’s Annulus Fibrosus: A Scaffold-Based Review Study

Tavakoli

2017

Tissue Eng Regen Med

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Tissue engineering as a high technology solution for treating disc's problem has been the focus of some researches recently; however, the upcoming successful results in this area depends on understanding the complexities of biology and engineering interface. Whereas the major responsibility of the nucleus pulposus is to provide a sustainable hydrated environment within the disc, the function of the annulus fibrosus (AF) is more mechanical, facilitating joint mobility and preventing radial bulging by confining of the central part, which makes the AF reconstruction important. Although the body of knowledge regarding the AF tissue engineering has grown rapidly, the opportunities to improve current understanding of how artificial scaffolds are able to mimic the AF concentric structure-including inter-lamellar matrix and cross-bridges-addressed unresolved research questions. The aim of this literature review was to collect and discuss, from the international scientific literature, information about tissue engineering of the AF based on scaffold fabrication and material properties, useful for developing new strategies in disc tissue engineering. The key parameter of this research was understanding if role of cross-bridges and inter-lamellar matrix has been considered on tissue engineering of the AF.

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Section: Biomaterials Selectionmentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Tissue Engineering of the Intervertebral Disc’s Annulus Fibrosus: A Scaffold-Based Review Study

Tavakoli

2017

Tissue Eng Regen Med

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“…To investigate influence of these extracellular matrix proteins on BMSCs based AF tissue engineering, a simple engineered AF tissue model is desired. Pellets culture in some studies have provide a three dimensional microenvironment as a simple investigation tool for AF tissue development [12,13] but they lack of the native tissue micro-architectures and hence may compromise its investigation. The advances in biomaterial scaffolds have create opportunities to recapitulate the micro-architecture of native AF tissue [9,[14][15][16] but most of these strategies face challenges with time consuming and complex manufacturing procedures as an investigation tool in AF engineering [9,[14][15][16].…”

Section: Introductionmentioning

confidence: 99%

The co-influence of hyaluronic acid and collagen on the development of an engineered annulus tissue model with bone marrow stromal cells

Chuah

Chia

et al. 2022

Biomed. Mater.

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Inveterbral disc degeneration is a significant musculoskeletal disease that brings huge burden of pain, disability, psychological and social consequences to the affected population worldwide with treatments that only alleviate the pain but does not address the underlying biological problems. For the past decades, tissue engineering of the disc has been investigated with Annulus Fibrosus been one of the complicated disc component to be engineered. With the limited source of annulus cells, bone marrow stromal cells have been frequently investigated as a potental cell candidate to develop an annulus fibrosus-like tissue which often require a multi-disclipinary effort to achieve. The extracellular matrix of Annulus Fibrosus is largely make up of collagen and proteoglycan which is still unclear how these matrix proteins could influence the bone marrow stromal cells towards constructing a AF-like tissue. In this study, we adopted a coiled hydrogel microfiber that resembles the micro-architecture of the native AF tissue to encapsulate bone marrow stromal cells and incorporated collagen type 1 and hyaluronic acid which later demonstrated that the co-presence of hyaluronic acid and collagen could potentially upregulated AF-associated biomarkers and protease expression which are critical for later development of an engineered AF tissue construct.

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“…It is well established that the NP contains NP resident stem/progenitor cells (NPScs), which serve a major role in maintaining cellular homeostasis and regeneration following aging and injury (8). NPScs have been found to maintain multipotent and self-renewal potential when cultured in vitro (9,10), and the resilience of the IVd cell population may be ensured if the relevant stem cell populations give rise to differentiated progeny over a person's lifetime (11,12). Although the cellular identity of NPScs that reside in the degenerated IVD remains poorly defined, numerous studies have reported that stem/progenitor cells expressing mesenchymal stem cell (MSc) markers have been isolated successfully from the degenerated IVd (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration

Shang

et al. 2018

Int J Mol Med

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Numerous studies have demonstrated the presence of resident nucleus pulposus stem/progenitor cells (NPSCs) in the tissue of the intervertebral disc (IVD). However, the cellular identity of NPSCs during IVD degeneration and ageing are poorly defined at present, despite significant progress in the understanding of NPSC biology. In the present study, NPSCs were isolated from human degenerated IVD and were characterized by flow cytometry, gene expression assays and proliferation and multipotency analysis. The results of the present study demonstrated that NPSCs isolated from human degenerated IVD may be divided into two groups according to the expression of mesenchymal stem cell (MSC) surface markers: The high expression of MSC surface markers group (H‑NPSCs) was highly positive for CD29, CD44, CD73, CD90 and CD105 at rates >95%, and the low expression of MSC markers surface markers group (L‑NPSCs), with the expression of CD29 and CD105 exhibiting individual variability, however, all at rates <95%. The donors for H‑NPSCs were aged <20 years, while the majority of donors for L‑NPSCs were aged >25 years, with one exception aged <20 years. The results highlighted that the low expression of MSC surface markers in NPSCs from aged and degenerated NP tissues were associated with a low rate of proliferation and reduced differentiation potential, as well as downregulation of the NP progenitor marker Tie2 and higher expression of NP cell‑specific markers. These findings demonstrated that the regenerative potential of human NPSCs declines with ageing and degeneration of the IVD.

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Three-dimensional development of tensile pre-strained annulus fibrosus cells for tissue regeneration: An in-vitro study

Cited by 7 publications

References 31 publications

Tissue Engineering of the Intervertebral Disc’s Annulus Fibrosus: A Scaffold-Based Review Study

Tissue Engineering of the Intervertebral Disc’s Annulus Fibrosus: A Scaffold-Based Review Study

The co-influence of hyaluronic acid and collagen on the development of an engineered annulus tissue model with bone marrow stromal cells

Regenerative potential of human nucleus pulposus resident stem/progenitor cells declines with ageing and intervertebral disc degeneration

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