Three-Dimensional Cell and Tissue Patterning in a Strained Fibrin Gel System

Matsumoto, Takuya; Sasaki, Jun; Alsberg, Eben; Egusa, Hiroshi; Yatani, Hirofumi; Sohmura, Taiji

doi:10.1371/journal.pone.0001211

Cited by 100 publications

(87 citation statements)

References 27 publications

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“…Therefore, frequently cell lines are used such as C2C12, which are satellite cells of C3H mice (Dennis et al, 2001;Borschel et al, 2004;Levenberg et al, 2005;Riboldi et al, 2005;Huang et al, 2006a;Boontheekul et al, 2007;Matsumoto et al, 2007). Also, satellite cells harvested from the soleus muscle of rats are used for TE of muscle tissue (Dennis and Kosnik, 2000;Beier et al, 2004;Stern-Straeter et al, 2005;Das et al, 2006;Borschel et al, 2006;Larkin et al, 2006;Bach et al, 2006;Huang et al, 2006b;Boontheekul et al, 2007).…”

Section: Progenitor Cellsmentioning

confidence: 99%

“…Nanoscale features are more efficient in promoting the assembly of longer myotubes than microscale features (Huang et al, 2006a). Parallel alignment can also be induced by applying mechanical or magnetic strain: muscle fibres reorganize along the line of force (Sekine et al, 2006;Matsumoto et al, 2007) and through static magnetic fields (Coletti et al, 2007).…”

Section: Scaffoldsmentioning

confidence: 99%

“…Another natural biodegradable 3D scaffold can be created by using fibrin. Satellite cells are mixed with a growth medium, fibrin and thrombin to create a fibrin gel (Beier et al, 2004(Beier et al, , 2006Stern-Straeter et al, 2005;Bach et al, 2006;Matsumoto et al, 2007). Muscle progenitor cells produce their own ECM proteins and over 3-4 weeks degrade and replace the original fibrin matrix (Huang et al, 2005), which is important because myoblasts proliferate more extensively in fast-degrading gels (Boontheekul et al, 2007).…”

Section: Scaffoldsmentioning

confidence: 99%

See 2 more Smart Citations

Current opportunities and challenges in skeletal muscle tissue engineering

Koning¹,

Harmsen²,

Luyn³

et al. 2009

J Tissue Eng Regen Med

144

108

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The purpose of this article is to give a concise review of the current state of the art in tissue engineering (TE) of skeletal muscle and the opportunities and challenges for future clinical applicability. The endogenous progenitor cells of skeletal muscle, i.e. satellite cells, show a high proneness to muscular differentiation, in particular exhibiting the same characteristics and function as its donor muscle. This suggests that it is important to use an appropriate progenitor cell, especially in TE facial muscles, which have a exceptional anatomical and fibre composition compared to other skeletal muscle. Muscle TE requires an instructive scaffold for structural support and to regulate the proliferation and differentiation of muscle progenitor cells. Current literature suggests that optimal scaffolding could comprise of a fibrin gel and cultured monolayers of muscle satellite cells obtained through the cell sheet technique. Tissue-engineered muscle constructs require an adequate connection to the vascular system for efficient transport of oxygen, carbon dioxide, nutrients and waste products. Finally, functional and clinically applicable muscle constructs depend on adequate neuromuscular junctions with neural cells. To reach this, it seems important to apply optimal electrical, chemotropic and mechanical stimulation during engineering and discover other factors that influence its formation. Thus, in addition to approaches for myogenesis, we discuss the current status of strategies for angiogenesis and neurogenesis of TE muscle constructs and the significance for future clinical use.

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Section: Progenitor Cellsmentioning

confidence: 99%

Section: Scaffoldsmentioning

confidence: 99%

Section: Scaffoldsmentioning

confidence: 99%

See 1 more Smart Citation

Current opportunities and challenges in skeletal muscle tissue engineering

Koning¹,

Harmsen²,

Luyn³

et al. 2009

J Tissue Eng Regen Med

144

108

View full text Add to dashboard Cite

show abstract

“…53 However, Matsumoto et al reported that after applying a continuous mechanical force, clots displayed bundle-like structures with fibrils that were oriented parallel to the strain direction. 77 In a later work by the same group, bone marrow-derived stromal cells (BMSCs) were cultured in a strained fibrin gel and exhibited a specific orientation which was identical to the strain direction, suggesting that structural changes in the gel provided contact guidance cues to promote cell alignment. Moreover, the direction of the cell proliferation as well as mineral deposition in the strained gel was restricted to the same direction.…”

mentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

368

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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“…Specifically, cyclic mechanical stretching of cell-seeded scaffolds has been shown to improve myoblast alignment, myofiber diameter, and skeletal muscle construct hypertrophy (22,23). Importantly, mechanically loaded tissueengineered skeletal muscle constructs often more closely mimic the tissue elasticity, structural organization, and force generation capabilities of native skeletal muscle (24)(25)(26). In addition, there is some clinical evidence that massage therapy or the physical manipulation of injured skeletal muscle and connective tissue may reduce pain and promote recovery by increasing blood flow (27)(28)(29).…”

mentioning

confidence: 99%

Biologic-free mechanically induced muscle regeneration

Cezar

Roche

Vandenburgh

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

150

125

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Severe skeletal muscle injuries are common and can lead to extensive fibrosis, scarring, and loss of function. Clinically, no therapeutic intervention exists that allows for a full functional restoration. As a result, both drug and cellular therapies are being widely investigated for treatment of muscle injury. Because muscle is known to respond to mechanical loading, we investigated instead whether a material system capable of massage-like compressions could promote regeneration. Magnetic actuation of biphasic ferrogel scaffolds implanted at the site of muscle injury resulted in uniform cyclic compressions that led to reduced fibrous capsule formation around the implant, as well as reduced fibrosis and inflammation in the injured muscle. In contrast, no significant effect of ferrogel actuation on muscle vascularization or perfusion was found. Strikingly, ferrogel-driven mechanical compressions led to enhanced muscle regeneration and a ∼threefold increase in maximum contractile force of the treated muscle at 2 wk compared with no-treatment controls. Although this study focuses on the repair of severely injured skeletal muscle, magnetically stimulated bioagent-free ferrogels may find broad utility in the field of regenerative medicine.

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Three-Dimensional Cell and Tissue Patterning in a Strained Fibrin Gel System

Cited by 100 publications

References 27 publications

Current opportunities and challenges in skeletal muscle tissue engineering

Current opportunities and challenges in skeletal muscle tissue engineering

A review of fibrin and fibrin composites for bone tissue engineering

Biologic-free mechanically induced muscle regeneration

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