Three-dimensional bioprinting of complex cell laden alginate hydrogel structures

Tabriz, Atabak Ghanizadeh; Hermida, Miguel A.; Leslie, Nicholas R.; Shu, Wenmiao

doi:10.1088/1758-5090/7/4/045012

Cited by 339 publications

(236 citation statements)

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“…[33,34] The nozzle geometry and the www.advancedsciencenews.com www.advhealthmat.de rheological properties of the applied hydrogel were identified as the most powerful driving forces that determine the level of shear stress in a dispensing system. [35,36] In the present study, we have shown that chemical modification of hydrogels, such as carboxylation, is an effective method to tune the rheological properties of a bioink in order to reduce dispensation associated shear stress-induced cell death. As an alternative to changing the material properties, the printing process can be adjusted to minimize shear stress.…”

Section: −1mentioning

confidence: 87%

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Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

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Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

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COMMUNICATION (1 of 7)© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Mechanically Tunable Bioink for 3D Bioprinting of Human CellsAurelien Forget, Andreas Blaeser, Florian Miessmer, Marius Köpf, Daniela F. Duarte Campos, Nicolas H. Voelcker, Anton Blencowe, Horst Fischer,* and V. Prasad Shastri* DOI: 10.1002/adhm.201700255 medium-the bioink. [5] The former approach offers the advantage that the 3D scaffold does not have to be fabricated under cytocompatible conditions. Therefore, a broader range of materials can be employed, for example, thermoplasts, such as poly(caprolactone), [6] and additionally, other alternative scaffold fabrication techniques, such as electrospinning, or pressurized gyration can be combined with subsequent functionalization of the scaffold with proteins. [7] In contrast, the latter approach, that is, direct fabrication of cellloaded constructs by hydrogel molding or 3D printing, places high demands on the cytocompatibility of the material and the fabrication process and the resolution is limited by the size of the extrusion nozzle utilized in the fabrication process. [8] However, the advantage of direct fabrication techniques, especially of 3D bioprinting, is its ability to generate constructs with spatially defined cell and material composition. Moreover, biomaterials that are conventionally used in cell culture such as alginate, [9] Pluronic, [10] gelatin, [11] nanocellulose, [12] self-assembling peptides, [13] and agarose [14] are highly advantageous for direct cell printing as they are soluble in water and hence can be formulated as a cell carrier. There has been extensive effort to build on and improve the properties of watersoluble polymers as bioinks. [15,16] For example, to overcome the limitation of the solubilization of Pluronic and its limited diversity of mechanical properties, blending of Pluronic with alginate [17] and crosslinking using acrylate-modified Pluronic have been explored. [10] Notwithstanding these advances that utilize chemical crosslinking to control the mechanical properties of the bioinks, controlling the shear behavior and mechanical This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels can be tuned over several orders of magnitudes (5-230 Pa) while ensuring almost no change to the shear viscosity (10-17 mPa) of the bioink solution; thus enabling the fabrication of 3D structures made of different mechanical domains under identical printing parameters and low nozzle shear stress. Human mesenchymal stem cells printed using CA as a bioink show significantly higher survival (95%) in comparison to when printed using native agarose (62%), a commonly used thermogelling hydrogel for 3D-bioprinting applications. This work paves the way toward the printing of complex tissue-like structures composed of a range of mechanically discrete microdomains that could potent...

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Section: −1mentioning

confidence: 87%

“…This will invariably induce a shear stress, which can be detrimental to the cell viability. [35] The more viscous the solution is, the higher will be the shear stress. Therefore, having a bioink with a low viscosity would be beneficial for techniques that require an extrusion of cell suspension within a biomaterial.…”

Section: −1mentioning

confidence: 99%

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Forget

Blaeser

Miessmer

et al. 2017

Adv Healthcare Materials

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“…Mechanically stable alginate scaffolds can be successfully fabricated using CaCl2 solution at higher concentrations (You et al, 2016a), but the incorporated cells can be adversely affected (Cao et al, 2012). Therefore, extruding the alginate precursor into a lower concentration CaCl2 solution has been recommended to limit effects on cell viability (Tabriz et al, 2015). Cellincorporated scaffolds should also be crosslinked immediately after printing to prevent significant decreases in cell viability (Cao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Naghieh

Karamooz-Ravari

Sarker

et al. 2018

Journal of the Mechanical Behavior of Biomedical Materials

102

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Tissue scaffolds fabricated by three-dimensional (3D) bioprinting are attracting considerable attention for tissue engineering applications. Because the mechanical properties of hydrogel scaffolds should match the damaged tissue, changing various parameters during 3D bioprinting has been studied to manipulate the mechanical behavior of the resulting scaffolds. Crosslinking scaffolds using a cation solution (such as CaCl 2 ) is also important for regulating the mechanical properties, but has not been well documented in the literature. Here, the effect of varied crosslinking agent volume and crosslinking time on the mechanical behavior of 3D bioplotted alginate scaffolds was evaluated using both experimental and numerical methods. Compression tests were used to measure the elastic modulus of each scaffold, then a finite element model was developed and a power model used to predict scaffold mechanical behavior. Results showed that crosslinking time and volume of crosslinker both play a decisive role in modulating the mechanical properties of 3D bioplotted scaffolds. Because mechanical properties of scaffolds can affect cell response, the findings of this study can be implemented to modulate the elastic modulus of scaffolds according to the intended application.

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“…Bingham plastic [2], high-density hydrophobic fluorocarbons [5] or more rigid biodegradable materials are used. A novel bioprinting scheme has been developed by Ghanizadeh Tabriz et al [6] wherein 3D cell-laden alginate structures are built up using a three-stage cross-linking process. Partially cross-linked alginate is extruded onto a porous PMMA platform which is lowered into a bath of 100mM calcium chloride solution as each layer is completed thus further cross-linking the structure before finally treating the completed structure with barium chloride in order to extend the degradation time of the hydrogel.…”

Section: Fugitive Support Bathsmentioning

confidence: 99%

“…Bioprinting vascularization can be divided in two groups: bioprinting the actual vessels [55] and bioprinting tissue with nutrient channels [54]. By building up a vessel layer by layer, it is possible to create the intricate structures of hollow, branched blood vessels [6,56] and larger self-supporting structures [57]. Hinton et al [2] showed they can create an entire, full-size, perfusable human right-hand coronary arterial tree out of alginate using FRESH printing.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Current developments in 3D bioprinting for tissue engineering

Cornelissen

Faulkner-Jones

Shu

2017

Current Opinion in Biomedical Engineering

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This version is available at https://strathprints.strath.ac.uk/61660/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPTCurrent developments in 3D bioprinting for tissue engineering AbstractThe field of 3-dimensional (3D) bioprinting have enjoyed rapid development in the past few years for the applications in tissue engineering and regenerative medicine. In this review, we summarize the most updated developments in 3D bioprinting for the applications in the tissue engineering with a focus on the printable biomaterials used as bioinks. These developments include 1) novel printing regimes have been enabled by the use of fugitive inks for the creation of intricate structures e.g. vascularized tissue constructs; 2) mechanical strength of printed constructs can be enhanced by co-printing soft and hard biomaterials; 3) bioprinted in-vitro models for drug testing applications are closer to reality. We conclude that the research and application of new bioinks will remain the key highlights of the future developments in 3D bioprinting for tissue engineering.

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Three-dimensional bioprinting of complex cell laden alginate hydrogel structures

Cited by 339 publications

References 38 publications

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Mechanically Tunable Bioink for 3D Bioprinting of Human Cells

Influence of crosslinking on the mechanical behavior of 3D printed alginate scaffolds: Experimental and numerical approaches

Current developments in 3D bioprinting for tissue engineering

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