Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Wu, Tong; Gao, Yujie; Su, Jin; Tang, Xiangyu; Chen, Qili; W, L; Zhang, J J; Wu, Jinglan; Wang, S X

doi:10.1080/13697137.2021.1921726

Cited by 38 publications

(20 citation statements)

References 53 publications

(58 reference statements)

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“…The hydrogel completely restricted the ovulation path; therefore, the oocyte was usually isolated by mechanical dissociation or enzymic digestion. In contrast to the previous 3D printed ovarian scaffold 50,51 , our micro-cavity ovary directly proved to restore the ovulation ability and supported the maturation of the individual follicle instead of a cluster of multiple follicles. The whole ovulation process could be entirely monitored under a microscope which may assist in investigating the unknown mechanism of reproductive diseases, such as the appearance of atretic follicles.…”

Section: Discussioncontrasting

confidence: 58%

Recapitulation of folliculogenesis to ovulation in a biomimetic micro-cavity ovary

Shan

et al. 2022

Preprint

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Ovary disease is a major cause of female infertility and could disrupt the hormone balance, which may cause multi-organ disorders in the long-term effect. However, conventional artificial ovary techniques usually encapsulate follicles in 3D hydrogels and block the ovulation ability. In this study, we fabricated a biomimetic micro-cavity ovary by a microsphere-templated technique to recapitulate the ovulation process, where sacrificial gelatin microspheres were mixed with photo-crosslinkable gelatin methacryloyl (GelMA) to engineer an open cavity niche for follicle growth. The microcavity’s parameters were optimized to be suitable for follicle growth. With that, we showed that the micro-cavity ovary could support the follicle growth to antral stage and the ovulation of meiosis-matured oocytes out of the micro-cavity ovary without extra manipulation. The ovulated oocyte was characterized in a great quality. This technique would be of great advantage to be further applied in clinics that recover natural conception after in vivo transplantation of the biomimetic ovary.

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Section: Discussioncontrasting

confidence: 58%

Recapitulation of folliculogenesis to ovulation in a biomimetic micro-cavity ovary

Shan

et al. 2022

Preprint

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“…This study highlighted the need to consider the immunogenic properties of biomaterials to match hydrogel models for their appropriate application, and may have implications on future 3D in vitro immunotherapy models. In the 3D bioprinting field, ovarian cancer cell lines have to date only been used to test printability of GelMA with extrusion based bioprinting, prior to testing its biocompatibility with murine oocytes ( Wu et al, 2021 ).…”

Section: 3d In Vitro Ovarian Cancer Modelsmentioning

confidence: 99%

“…Extrusion based bioprinting has been employed to fabricate a 3D bioprinted ovary that successfully supported oocyte growth using a GelMA-based bioink ( Wu et al, 2021 ). While this study focused on oocyte maturation ex vivo , ovarian cancer cell lines were utilized during optimization of bioink biocompatibility, showing high cell viability during and after the extrusion process.…”

Section: 3d In Vitro Ovarian Cancer Modelsmentioning

confidence: 99%

Three-Dimensional Modelling of Ovarian Cancer: From Cell Lines to Organoids for Discovery and Personalized Medicine

Yee

Dickson

Muntasir

et al. 2022

Front. Bioeng. Biotechnol.

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Ovarian cancer has the highest mortality of all of the gynecological malignancies. There are several distinct histotypes of this malignancy characterized by specific molecular events and clinical behavior. These histotypes have differing responses to platinum-based drugs that have been the mainstay of therapy for ovarian cancer for decades. For histotypes that initially respond to a chemotherapeutic regime of carboplatin and paclitaxel such as high-grade serous ovarian cancer, the development of chemoresistance is common and underpins incurable disease. Recent discoveries have led to the clinical use of PARP (poly ADP ribose polymerase) inhibitors for ovarian cancers defective in homologous recombination repair, as well as the anti-angiogenic bevacizumab. While predictive molecular testing involving identification of a genomic scar and/or the presence of germline or somatic BRCA1 or BRCA2 mutation are in clinical use to inform the likely success of a PARP inhibitor, no similar tests are available to identify women likely to respond to bevacizumab. Functional tests to predict patient response to any drug are, in fact, essentially absent from clinical care. New drugs are needed to treat ovarian cancer. In this review, we discuss applications to address the currently unmet need of developing physiologically relevant in vitro and ex vivo models of ovarian cancer for fundamental discovery science, and personalized medicine approaches. Traditional two-dimensional (2D) in vitro cell culture of ovarian cancer lacks critical cell-to-cell interactions afforded by culture in three-dimensions. Additionally, modelling interactions with the tumor microenvironment, including the surface of organs in the peritoneal cavity that support metastatic growth of ovarian cancer, will improve the power of these models. Being able to reliably grow primary tumoroid cultures of ovarian cancer will improve the ability to recapitulate tumor heterogeneity. Three-dimensional (3D) modelling systems, from cell lines to organoid or tumoroid cultures, represent enhanced starting points from which improved translational outcomes for women with ovarian cancer will emerge.

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“…Previous studies have shown that GelMA/alginate hybrid hydrogels provided optimal mechanical performance and degradation rate while also supporting cell activity and growth. 43,49,50 As shown in Figure 2B, vii, the bioprinted tri-polymer structure based on GelMA, gelatin, and alginate maintained its shape fidelity even after incubation for 21 days, which confirmed the tri-polymer hydrogel provided sufficient structural support for bioprinting and cell culture.…”

Section: Mechanical Properties and Swelling Behaviormentioning

confidence: 64%

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Shahabipour

Tavafoghi

Aninwene

et al. 2022

J Biomedical Materials Res

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The crosstalk between osteoblasts and endothelial cells is critical for bone vascularization and regeneration. Here, we used a coaxial 3D bioprinting method to directly print an osteon-like structure by depositing angiogenic and osteogenic bioinks from the core and shell regions of the coaxial nozzle, respectively. The bioinks were made up of gelatin, gelatin methacryloyl (GelMA), alginate, and hydroxyapatite (HAp) nanoparticles and were loaded with human umbilical vascular endothelial cells (HUVECs) and osteoblasts (MC3T3) in the core and shell regions, respectively. Conventional monoaxial 3D bioprinting was used as a control method, where the hydrogels, HAp nanoparticles, MC3T3 cells, and HUVECs were all mixed in one bioink and printed from the core nozzle. As a result, the bioprinted scaffolds were composed of cell-laden fibers with either a core-shell or homogenous structure, providing a noncontact (indirect) or contact (direct) co-culture of MC3T3 cells and HUVECs, respectively. Both structures supported the 3D culture of HUVECs and osteoblasts over a long period. The scaffolds also supported the expression of osteogenic and angiogenic factors. However, the gene expression was significantly higher for the coreshell structure than the homogeneous structure due to the well-defined distribution of osteoblasts and endothelial cells and the formation of vessel-like structures in the co-culture system. Our results indicated that the coaxial bioprinting technique, with the ability to create a non-contact co-culture of cells, can provide a more efficient bioprinting strategy for printing highly vascularized and bioactive bone structures.

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Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Cited by 38 publications

References 53 publications

Recapitulation of folliculogenesis to ovulation in a biomimetic micro-cavity ovary

Recapitulation of folliculogenesis to ovulation in a biomimetic micro-cavity ovary

Three-Dimensional Modelling of Ovarian Cancer: From Cell Lines to Organoids for Discovery and Personalized Medicine

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

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