Coaxial <scp>3D</scp> bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Shahabipour, Fahimeh; Tavafoghi, Maryam; Aninwene, George E.; Bonakdar, Shahin; Oskuee, Reza Kazemi; Shokrgozar, Mohammad Ali; Potyondy, Tyler; Alambeigi, Farshid; Ahadian, Samad

doi:10.1002/jbm.a.37354

Cited by 21 publications

(11 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One approach to induce vessel formation is a coculture with other cells, for example, osteoblasts. It has been well described that these cells influence each other and enhance osteogenesis as well as angiogenesis [ 23 , 52 , 53 ]. Therefore, the spheroid model experiment was performed with a coculture of osteoblasts and endothelial cells.…”

Section: Resultsmentioning

confidence: 99%

Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo

et al. 2023

View full text Add to dashboard Cite

Bone fracture healing is a multistep process, including early immunological reactions, osteogenesis, and as a key factor, angiogenesis. Molecules inducing osteogenesis as well as angiogenesis are rare, but hold promise to be employed in bone tissue engineering. It has been demonstrated that the bone sialoprotein (BSP) can induce bone formation when immobilized in collagen type I, but its effect on angiogenesis still has to be characterized in detail. Therefore, the aim of this study was to analyse the effects of BSP immobilized in a collagen type I gel on angiogenesis. First, in vitro analyses with endothelial cells (HUVECs) were performed detecting enhancing effects of BSP on proliferation and gene expression of endothelial markers. A spheroid model was employed confirming these results. Finally, the inducing impact of BSP-collagen on vascular density was proved in a yolk sac membrane assay. Our results demonstrate that BSP is capable of inducing angiogenesis and confirm that collagen type I is the optimal carrier for this protein. Taking into account former results, and literature showing that BSP also induces osteogenesis, one can hypothesize that BSP couples angiogenesis and osteogenesis, making it a promising molecule to be used in bone tissue regeneration.

show abstract

Section: Resultsmentioning

confidence: 99%

Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Autologous stem cells appear to be the most viable option for in vivo implantation of the 3D printed vascular tissues/organs in the future. In recent years, co-printing of ECs with MSCs and other adult cells have become more and more prominent for vascualr tissue/organ construction [37,[198][199][200]. With the development of bioprinting technologies, most of the researchers tend to choose MSCs as the cellular components in their bioinks [201].…”

Section: Cellsmentioning

confidence: 99%

Bioprinting Technologies and Bioinks for Vascular Model Establishment

Kong

Wang

2023

IJMS

View full text Add to dashboard Cite

Clinically, large diameter artery defects (diameter larger than 6 mm) can be substituted by unbiodegradable polymers, such as polytetrafluoroethylene. There are many problems in the construction of small diameter blood vessels (diameter between 1 and 3 mm) and microvessels (diameter less than 1 mm), especially in the establishment of complex vascular models with multi-scale branched networks. Throughout history, the vascularization strategies have been divided into three major groups, including self-generated capillaries from implantation, pre-constructed vascular channels, and three-dimensional (3D) printed cell-laden hydrogels. The first group is based on the spontaneous angiogenesis behaviour of cells in the host tissues, which also lays the foundation of capillary angiogenesis in tissue engineering scaffolds. The second group is to vascularize the polymeric vessels (or scaffolds) with endothelial cells. It is hoped that the pre-constructed vessels can be connected with the vascular networks of host tissues with rapid blood perfusion. With the development of bioprinting technologies, various fabrication methods have been achieved to build hierarchical vascular networks with high-precision 3D control. In this review, the latest advances in 3D bioprinting of vascularized tissues/organs are discussed, including new printing techniques and researches on bioinks for promoting angiogenesis, especially coaxial printing, freeform reversible embedded in suspended hydrogel printing, and acoustic assisted printing technologies, and freeform reversible embedded in suspended hydrogel (flash) technology.

show abstract

“…11 Moreover, to simulate the organization of bone tissue and enhance cellular connections, the co-culture of vascular cells and osteogenic cells is essential. 12 The employment of the coculture methodology has demonstrated a remarkable potential for angiogenesis and osteogenesis. 13,14 When fabricating large-size tissue constructs, the exchange of nutrients and metabolic wastes needs to be carefully considered.…”

Section: Introductionmentioning

confidence: 99%

“…The osteogenic and angiogenic potentials of independently encapsulated MC3T3 preosteoblasts and HUVEC using coaxial bioprinting have been demonstrated to surpass that of encapsulating the two types of cells in a homogeneous bioink by monoaxial bioprinting. 12 Additionally, the incorporation of nutrient channels facilitates the exchange of nutrients and cellular metabolic byproducts. Therefore, triaxial bioprinting technology not only has the capacity to mimic osteogenic and angiogenic parts, but it also establishes nutrient channels.…”

Section: Introductionmentioning

confidence: 99%

“…It can facilitate vascular tissue growth from grafts into the host tissue to complement vascular ingrowth from the host into grafts 11 . Moreover, to simulate the organization of bone tissue and enhance cellular connections, the co‐culture of vascular cells and osteogenic cells is essential 12 . The employment of the co‐culture methodology has demonstrated a remarkable potential for angiogenesis and osteogenesis 13,14 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Zhang,

Suttapreyasri,

Leethanakul

et al. 2024

J Biomedical Materials Res

View full text Add to dashboard Cite

Bone tissue is a highly vascularized tissue. When constructing tissue‐engineered bone models, both the osteogenic and angiogenic capabilities of the construct should be carefully considered. However, fabricating a vascularized tissue‐engineered bone to promote vascular formation and bone generation, while simultaneously establishing nutrition channels to facilitate nutrient exchange within the constructs, remains a significant challenge. Triaxial bioprinting, which not only allows the independent encapsulation of different cell types while simultaneously forming nutrient channels, could potentially emerge as a strategy for fabricating vascularized tissue‐engineered bone. Moreover, bioinks should also be applied in combination to promote both osteogenesis and angiogenesis. In this study, employing triaxial bioprinting, we used a blend bioink of gelatin methacryloyl (GelMA), sodium alginate (Alg), and different concentrations of nano beta‐tricalcium phosphate (nano β‐TCP) encapsulated MC3T3‐E1 preosteoblasts as the outer layer, a mixed bioink of GelMA and Alg loaded with human umbilical vein endothelial cells (HUVEC) as the middle layer, and gelatin as a sacrificial material to form nutrient channels in the inner layer to fabricate vascularized bone constructs simulating the microenvironment for bone and vascular tissues. The results showed that the addition of nano β‐TCP could adjust the mechanical, swelling, and degradation properties of the constructs. Biological assessments revealed the cell viability of constructs containing different concentrations of nano β‐TCP was higher than 90% on day 7, The cell‐laden constructs containing 3% (w/v) nano β‐TCP exhibited better osteogenic (higher Alkaline phosphatase activity and larger Osteocalcin positive area) and angiogenic (the gradual increased CD31 positive area) potential. Therefore, using triaxial bioprinting technology and employing GelMA, Alg, and nano β‐TCP as bioink components could fabricate vascularized bone tissue constructs, offering a novel strategy for vascularized bone tissue engineering.

show abstract

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Cited by 21 publications

References 71 publications

Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo

Bone Sialoprotein Immobilized in Collagen Type I Enhances Angiogenesis In Vitro and In Ovo

Bioprinting Technologies and Bioinks for Vascular Model Establishment

Fabrication of vascularized tissue‐engineered bone models using triaxial bioprinting

Contact Info

Product

Resources

About