Three-Dimensional Bioprinting Applications for Bone Tissue Engineering

Maresca, Jamie A.; DeMel, Derek C.; Wagner, Grayson A.; Haase, Colin; Geibel, John P.

doi:10.3390/cells12091230

Cited by 14 publications

(4 citation statements)

References 79 publications

(194 reference statements)

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“…Simultaneously, we noticed that locking nail failure mainly occurred at the distal end of osteotomy during simulation. This was mostly due to a difference in anatomical characteristic of the femur and the internal fixation apparatus (proximal end of the intramedullary nail thicker than the distal end, and the proximal end of the femoral bone marrow cavity thinner than the distal end) [ 23 ]. The press-fit between the intramedullary nail and the medullary cavity at the distal end of the femur was not as satisfactory as compared to the proximal end.…”

Section: Discussionmentioning

confidence: 99%

Computer-aided design and 3D printing for a stable construction of segmental bone defect model in Beagles: a short term observation

Cheng,

Zhu,

Peng

et al. 2024

3D Print Med

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Objective Segmental bone defect animal studies require stable fixation which is a continuous experimental challenge. Large animal models are comparable to the human bone, but with obvious drawbacks of housing and costs. Our study aims to utilize CAD and 3D printing in the construction of a stable and reproducible segmental bone defect animal mode. Methods CAD-aided 3D printed surgical instruments were incorporated into the construction of the animal model through preoperative surgical emulation. 20 3D printed femurs were divided into either experimental group using 3D surgical instruments or control group. In Vitro surgical time and accuracy of fixation were analysed and compared between the two groups. A mature surgical plan using the surgical instruments was then utilized in the construction of 3 segmental bone defect Beagle models in vivo. The Beagles were postoperatively assessed through limb function and imaging at 1, 2 and 3 months postoperatively. Results In vitro experiments showed a significant reduction in surgical time from 40.6 ± 14.1 (23–68 min) to 26 ± 4.6 (19–36 min) (n = 10, p < 0.05) and the accuracy of intramedullary fixation placement increased from 71.6 ± 23.6 (33.3–100) % to 98.3 ± 5.37 (83–100) %, (n = 30, p < 0.05) with the use of CAD and 3D printed instruments. All Beagles were load-bearing within 1 week, and postoperative radiographs showed no evidence of implant failure. Conclusion Incorporation of CAD and 3D printing significantly increases stability, while reducing the surgical time in the construction of the animal model, significantly affecting the success of the segmental bone defect model in Beagles.

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Section: Discussionmentioning

confidence: 99%

Computer-aided design and 3D printing for a stable construction of segmental bone defect model in Beagles: a short term observation

Cheng,

Zhu,

Peng

et al. 2024

3D Print Med

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“…These methods allow for the printing of bone constructs encapsulating living cells, growth factors, etc. They are essential for fabricating vascularized, functional bone grafts with a physiological cell distribution (Arastouei et al, 2021;Zhou et al, 2021;Maresca et al, 2023). Among the available techniques, FDM is the most widely used 3D printing technique for bone scaffolds due to its simplicity, low cost, and ability to process a range of biomaterials.…”

Section: Biological 3d Printingmentioning

confidence: 99%

Additively manufactured porous scaffolds by design for treatment of bone defects

Toosi,

Javid-Naderi,

Tamayol

et al. 2024

Front. Bioeng. Biotechnol.

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There has been increasing attention to produce porous scaffolds that mimic human bone properties for enhancement of tissue ingrowth, regeneration, and integration. Additive manufacturing (AM) technologies, i.e., three dimensional (3D) printing, have played a substantial role in engineering porous scaffolds for clinical applications owing to their high level of design and fabrication flexibility. To this end, this review article attempts to provide a detailed overview on the main design considerations of porous scaffolds such as permeability, adhesion, vascularisation, and interfacial features and their interplay to affect bone regeneration and osseointegration. Physiology of bone regeneration was initially explained that was followed by analysing the impacts of porosity, pore size, permeability and surface chemistry of porous scaffolds on bone regeneration in defects. Importantly, major 3D printing methods employed for fabrication of porous bone substitutes were also discussed. Advancements of MA technologies have allowed for the production of bone scaffolds with complex geometries in polymers, composites and metals with well-tailored architectural, mechanical, and mass transport features. In this way, a particular attention was devoted to reviewing 3D printed scaffolds with triply periodic minimal surface (TPMS) geometries that mimic the hierarchical structure of human bones. In overall, this review enlighten a design pathway to produce patient-specific 3D-printed bone substitutions with high regeneration and osseointegration capacity for repairing large bone defects.

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“…Research on the bioprinting of type I collagen has focused on hard tissue applications [17,18] such as the bone, teeth, and spine where the mechanical properties play a key role. Bone regeneration fails once it reaches a critical size defect; thus, 3D printing with collagen inks for hard tissue repair is a growing area of research.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Liquid Collagen Ink for Three-Dimensional Printing

Snider,

Glover,

Grant

et al. 2024

Micromachines

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Three-dimensional printing provides more versatility in the fabrication of scaffold materials for hard and soft tissue replacement, but a critical component is the ink. The ink solution should be biocompatible, stable, and able to maintain scaffold shape, size, and function once printed. This paper describes the development of a collagen ink that remains in a liquid pre-fibrillized state prior to printing. The liquid stability occurs due to the incorporation of ethylenediaminetetraacetic acid (EDTA) during dialysis of the collagen. Collagen inks were 3D-printed using two different printers. The resulting scaffolds were further processed using two different chemical crosslinkers, 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride)/N-hydroxysuccinimide (EDC/NHS) and genipin; gold nanoparticles were conjugated to the scaffolds. The 3D-printed scaffolds were characterized to determine their extrudability, stability, amount of AuNP conjugated, and overall biocompatibility via cell culture studies using fibroblast cells and stroma cells. The results demonstrated that the liquid collagen ink was amendable to 3D printing and was able to maintain its 3D shape. The scaffolds could be conjugated with gold nanoparticles and demonstrated enhanced biocompatibility. It was concluded that the liquid collagen ink is a good candidate material for the 3D printing of tissue scaffolds.

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Three-Dimensional Bioprinting Applications for Bone Tissue Engineering

Cited by 14 publications

References 79 publications

Computer-aided design and 3D printing for a stable construction of segmental bone defect model in Beagles: a short term observation

Computer-aided design and 3D printing for a stable construction of segmental bone defect model in Beagles: a short term observation

Additively manufactured porous scaffolds by design for treatment of bone defects

Investigation of Liquid Collagen Ink for Three-Dimensional Printing

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