Three-dimensional bioprinting and electrospinning of cellulose nanocrystal/polycaprolactone for tracheal scaffolds

Abstract

Three-dimensional printing (3DP) has high flexibility and controllability, and has attracted extensive attention in the biomedical field. However, the scaffolds prepared only by 3D bioprinting have poor mechanical properties, and they cannot effectively carry the required drugs. At the same time, compared with the size of cells, the pore size of 3D printed scaffolds is relatively large, and the efficiency of cell inoculation and tissue formation are still limited by the pore resolution of scaffolds. Therefore, a new method of forming 3D printing trachea composites is proposed. When combined, 3D bioprinting and electrospinning (ESP) can overcome the issues associated with scaffolds prepared by 3D bioprinting alone. Nanofibers create a suitable environment for cell growth. In terms of material use, Polycaprolactone (PCL) is commonly used as an ideal material source for 3D printing, but its biomechanical properties are insufficient. Cellulose nanocrystals (CNC) can effectively improve the properties of polymers such as PCL. Therefore, the inner layer of the scaffold used in tracheal surgery is created from PCL/CNC composite by 3D bioprinting, and the outer nano-cellulose film is deposited on the inner surface by electrospinning. Mechanical properties and cell adhesion/growth of scaffolds prepared by 3D bioprinting combined with electrospinning were found to be superior to those of scaffolds prepared by 3D bioprinting.