Three-dimensional analysis of mineralizing turkey leg tendon: Matrix vesicle-collagen relationships

Kohler, Derek M.; Crenshaw, M.A.; Arsenault, A. Larry

doi:10.1016/s0945-053x(05)80003-4

Cited by 7 publications

(3 citation statements)

References 35 publications

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“…Further, the enriched but not yet mineralized collagen fibrils support the hypothesis that the mineralization process of the collagen fibril can occur independently from matrix vesicle mineralization (Landis and Song 1991). Consequently, the sequence of mineralization needs not to start at the matrix vesicles and subsequently propagate to adjacent collagen fibrils and spread within the fibrils as suggested by Kohler et al (1994) for the mineralizing turkey leg tendon.…”

Section: Discussionsupporting

confidence: 55%

Sutural mineralization of rat calvaria characterized by atomic-force microscopy and transmission electron microscopy

Wiesmann

Chi

Stratmann

et al. 1998

Cell and Tissue Research

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The application of transmission electron microscopy (TEM) and atomic-force microscopy (AFM) aid the acquisition of detailed structural information on the process of hard tissue formation. The sutural mineralization of rat calvaria is taken as a model for a collagen-related mineralization system. After cryofixation or chemical fixation an anhydrous tissue preparation technique with no staining procedures is used. The atomic-force microscope and the transmission electron microscope are used for structural analysis of the mineralizing region of the sutural tissue. With the application of AFM the collagen macroperiod is shown to be well represented in the unmineralized sutural tissue. At the mineralization front the collagen fibrils are found to be thickened and to change to a characteristic stacked platelet structure. Using TEM the macroperiod is faintly visible before mineral crystallites have formed and is more prominent after the apatite crystallization has started in the fibrils. In this step a needle-like structure of the newly formed apatitic crystals is visible.

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Section: Discussionsupporting

confidence: 55%

Sutural mineralization of rat calvaria characterized by atomic-force microscopy and transmission electron microscopy

Wiesmann

Chi

Stratmann

et al. 1998

Cell and Tissue Research

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“…Mineralized parts of digital flexor tendons of domestic turkeys [1][2][3][19][20][21][22][23][24][25] older than 24 weeks were used in this study. Ten tendons were harvested from eight legs obtained from a local market.…”

Section: Sample Preparationmentioning

confidence: 99%

Influence of Mineralization and Microporosity on Tissue Elasticity: Experimental and Numerical Investigation on Mineralized Turkey Leg Tendons

2012

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A combined experimental and numerical study correlating indentation stiffness with mineralization and microporosity was performed on mineralized turkey leg tendon. Two distinct tissue morphologies were distinguished by quantitative backscattered electron imaging and called "circumferential" and "interstitial" zones. These two zones showed different tissue organization, microporosity, and mineralization. Stiffness, measured by microindentation, was also different in the two zones. The mean field method of modeling of mineralized collagen fibers was employed to explain the differences.

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“…The mineralized area develops to the lower side of the internal layer. It is believed that the mineralization of tendon and bone are assisted by matrix vesicles (Kohler et al, 1994), but the internal layer of fish scales are mineralized in the absence of matrix vesicles (Yamada & Watabe, 1979). The difference of mineralization mechanism should affect the mineralized structure of aligned collagen fibrils.…”

Section: Introductionmentioning

confidence: 99%

Minerals and Aligned Collagen Fibrils in Tilapia Fish Scales: Structural Analysis Using Dark-Field and Energy-Filtered Transmission Electron Microscopy and Electron Tomography

et al. 2011

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The mineralized structure of aligned collagen fibrils in a tilapia fish scale was investigated using transmission electron microscopy (TEM) techniques after a thin sample was prepared using aqueous techniques. Electron diffraction and electron energy loss spectroscopy data indicated that a mineralized internal layer consisting of aligned collagen fibrils contains hydroxyapatite crystals. Bright-field imaging, dark-field imaging, and energy-filtered TEM showed that the hydroxyapatite was mainly distributed in the hole zones of the aligned collagen fibrils structure, while needle-like materials composed of calcium compounds including hydroxyapatite existed in the mineralized internal layer. Dark-field imaging and three-dimensional observation using electron tomography revealed that hydroxyapatite and needle-like materials were mainly found in the matrix between the collagen fibrils. It was observed that hydroxyapatite and needle-like materials were preferentially distributed on the surface of the hole zones in the aligned collagen fibrils structure and in the matrix between the collagen fibrils in the mineralized internal layer of the scale.

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Three-dimensional analysis of mineralizing turkey leg tendon: Matrix vesicle-collagen relationships

Cited by 7 publications

References 35 publications

Sutural mineralization of rat calvaria characterized by atomic-force microscopy and transmission electron microscopy

Sutural mineralization of rat calvaria characterized by atomic-force microscopy and transmission electron microscopy

Influence of Mineralization and Microporosity on Tissue Elasticity: Experimental and Numerical Investigation on Mineralized Turkey Leg Tendons

Minerals and Aligned Collagen Fibrils in Tilapia Fish Scales: Structural Analysis Using Dark-Field and Energy-Filtered Transmission Electron Microscopy and Electron Tomography

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