Influence of Mineralization and Microporosity on Tissue Elasticity: Experimental and Numerical Investigation on Mineralized Turkey Leg Tendons

Spiesz, Ewa M.; Roschger, Paul; Zysset, Philippe

doi:10.1007/s00223-012-9578-5

Cited by 28 publications

(30 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Earlier studies on mineralization of the two zones of morphology had shown low heterogeneity of mineralization within a zone (Spiesz et al., 2012). This allowed defining additional statistical outliers of indentation moduli as values higher than 1.5 times the interquartile range above the third quartile and below the first quartile (Crawley, 2005).…”

Section: Methodsmentioning

confidence: 97%

“…A similar procedure was successfully used for bone sections preparation for indentation (Wolfram et al., 2010a,b). Two morphological zones present in MTLT (Spiesz et al., 2012), the circumferential and the interstitial, were investigated separately by indentation. The mineralization within a morphology zone measured using quantitative backscattered electron imaging was homogeneous.…”

Section: Methodsmentioning

confidence: 99%

“…The mineralization within a morphology zone measured using quantitative backscattered electron imaging was homogeneous. The interstitial zone showed a higher porosity than the circumferential one (Spiesz et al., 2012). …”

Section: Methodsmentioning

confidence: 99%

“…Constituents of MTLT are similar to those of bone, being mainly collagen, of which 90% is the type I collagen (Landis and Silver, 2002; Fritsch and Hellmich, 2007), the mineral — hydroxyapatite and water. The mineral content of tendons in animals 24 weeks old was reported to be slightly lower than that of human bone (Gupta et al., 2004), but within the bone mineralization level for tendons extracted from older turkeys (Spiesz et al., 2012). …”

Section: Introductionmentioning

confidence: 99%

“…One morphology, called the circumferential zone, consists of densely packed mineralized collagen fibers and is placed around large pores of the tendon (blood vessels). The other morphology, called the interstitial zone, is located between the large pores and has a more sparse structure and higher microporosity (Spiesz et al., 2012). The zones had different morphology and composition, and therefore were treated separately in this study.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Elastic anisotropy of uniaxial mineralized collagen fibers measured using two-directional indentation. Effects of hydration state and indentation depth

Spiesz

Roschger

Zysset

2012

Journal of the Mechanical Behavior of Biomedical Materials

Self Cite

View full text Add to dashboard Cite

Mineralized turkey leg tendon (MTLT) is an attractive model of mineralized collagen fibers, which are also present in bone. Its longitudinal structure is advantageous for the relative simplicity in modeling, yet its anisotropic elastic properties remain unknown. The aim of this study was to quantify the extent of elastic anisotropy of mineralized collagen fibers by using nano- and microindentation to probe a number on MTLT samples in two orthogonal directions. The large dataset allowed the quantification of the extent of anisotropy, depending on the final indentation depth and on the hydration state of the sample.Anisotropy was observed to increase with the sample re-hydration process. Artifacts of indentation in a transverse direction to the main axis of the mineralized tendons in re-hydrated condition were observed. The indentation size effect, that is, the increase of the measured elastic properties with decreasing sampling volume, reported previously on variety of materials, was also observed in MTLT. Indentation work was quantified for both directions of indentation in dried and re-hydrated conditions.As hypothesized, MTLT showed a higher extent of anisotropy compared to cortical and trabecular bone, presumably due to the alignment of mineralized collagen fibers in this tissue.

show abstract

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Elastic anisotropy of uniaxial mineralized collagen fibers measured using two-directional indentation. Effects of hydration state and indentation depth

Spiesz

Roschger

Zysset

2012

Journal of the Mechanical Behavior of Biomedical Materials

Self Cite

View full text Add to dashboard Cite

show abstract

Spherulitic Crystal Growth Drives Mineral Deposition Patterns in Collagen‐Based Materials

Macías‐Sánchez

Tarakina

Ivanov

et al. 2022

Adv Funct Materials

View full text Add to dashboard Cite

The formation of the hard tissues that provide support and mobility to organisms is achieved through the interplay of inorganic crystals and an organic framework composed of collagen and a small percentage of non‐collagenous proteins. Despite their clinical relevance, the mechanisms governing mineralization of the extracellular matrix are still poorly understood. By using 3D electron tomography and high‐resolution electron microscopy imaging and spectroscopy, it has been demonstrated that mineralization proceeds through a spherulitic‐like crystal growth process. First, aggregates of disordered crystals form in the interfibrillar spaces, which lead to the mineralization of adjacent fibrils. Mineral propagates steadily through the inter‐ and intrafibrillar spaces of the collagen structure forming layered spherulites that grow to confluence. The structure of the collagen fibrils serves as a protein scaffold to guide the formation of a myriad of platelet‐shaped crystallites that make up each of these spherulites. At their periphery, nanosized unmineralized areas remain, leading to the formation of the characteristic lacy pattern observed in the transversal cross‐section of mature calcified tissues. This study provides fundamental insights into the bone formation process and represents a potential strategy for complex materials design.

show abstract

Tendon mineralization is accelerated bilaterally and creep of contralateral tendons is increased after unilateral needle injury of murine achilles tendons

O'Brien

Shrive

Rosvold

et al. 2013

Journal Orthopaedic Research

View full text Add to dashboard Cite

Heterotopic mineralization may result in tendon weakness, but effects on other biomechanical responses have not been reported. We used a needle injury, which accelerates spontaneous mineralization of murine Achilles tendons, to test two hypotheses: that injured tendons would demonstrate altered biomechanical responses; and that unilateral injury would accelerate mineralization bilaterally. Mice underwent left hind (LH) injury (I; n ¼ 11) and were euthanized after 20 weeks along with non-injured controls (C; n ¼ 9). All hind limbs were examined by micro computed tomography followed by biomechanical testing (I ¼ 7 and C ¼ 6). No differences were found in the biomechanical responses of injured tendons compared with controls. However, the right hind (RH) tendons contralateral to the LH injury exhibited greater static creep strain and total creep strain compared with those LH tendons (p 0.045) and RH tendons from controls (p 0.043). RH limb lesions of injured mice were three times larger compared with controls (p ¼ 0.030). Therefore, despite extensive mineralization, changes to the responses we measured were limited or absent 20 weeks postinjury. These results also suggest that bilateral occurrence should be considered where tendon mineralization is identified clinically. This experimental system may be useful to study the mechanisms of bilateral new bone formation in tendinopathy and other conditions. ß

show abstract

Influence of Mineralization and Microporosity on Tissue Elasticity: Experimental and Numerical Investigation on Mineralized Turkey Leg Tendons

Cited by 28 publications

References 47 publications

Elastic anisotropy of uniaxial mineralized collagen fibers measured using two-directional indentation. Effects of hydration state and indentation depth

Elastic anisotropy of uniaxial mineralized collagen fibers measured using two-directional indentation. Effects of hydration state and indentation depth

Spherulitic Crystal Growth Drives Mineral Deposition Patterns in Collagen‐Based Materials

Tendon mineralization is accelerated bilaterally and creep of contralateral tendons is increased after unilateral needle injury of murine achilles tendons

Contact Info

Product

Resources

About