Three Closely Linked Genetic Systems Relevant to Transplantation

Yunis, E. J.; Amos, D. Bernard

doi:10.1073/pnas.68.12.3031

Cited by 271 publications

(95 citation statements)

References 18 publications

(20 reference statements)

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“…Any interpretation should take into account (a) that the allograft system used is modeled on a "one-way" mixed lymphocyte culture (MLC) using allogeneic lymphoid cells as stimulator cells and consider (b) the recent demonstration of a third locus (MLC locus) in the genetic region of the major histocompatibility complex (MHC) (28)(29)(30). Unlike the two loci (H-2K and H-2D in the mouse), which control serologically defined (H-2) antigens, the third locus (MLC locus) appears to be responsible for allogeneic differences that result in cell proliferation in the MLC, yet are not detectable serologically (lymphocyte-defined [LD] antigens).…”

Section: Discussionmentioning

confidence: 99%

Synergy During in Vitro Cytotoxic Allograft Responses

Wagner

1973

The Journal of Experimental Medicine

129

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The importance of cell interaction among lymphoid cells in the development of immune responses is well established. In humoral responses bone marrowderived (B) lymphocytes are recognized to be precursors of the cells that release serum antibody (1-4). Thymus-derived (T) lymphocytes, on the other hand, do not develop into antibody-forming cells (5) but play an important role in primary antibody responses by cooperating with B cells (6, 7).In contrast, cellular immunity is thought to be mediated by T lymphocytes, apparently without collaboration with B lymphocytes. However there is increasing evidence to suggest that cooperative interaction occurs among subsets of T lymphocytes (8-11). For example, Cantor and Asofsky proposed that a Ti-T~ cell interaction takes place during the initiation of graft-vs.-host responses in F1 neonates (8,9).Cytotoxic in vitro allograft responses have been shown to be mediated by T lymphocytes (12, 13). As such, they provide a convenient tool for analyzing the cellular requirements necessary for such a response to occur. Experiments therefore were designed to evaluate in vitro the concept of T-T cell interaction in cellular immunity against transplantation antigens. The results obtained are compatible with the idea that two cell types interact with each other in the initiation of such a response. Moreover, evidence is provided that one cell type acts mainly as helper cells allowing precursor cells of cytotoxic lymphocytes (CL) i to be immunized effectively.

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Section: Discussionmentioning

confidence: 99%

Synergy During in Vitro Cytotoxic Allograft Responses

Wagner

1973

The Journal of Experimental Medicine

129

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“…28,000, which are noncovalently associated (23,25,26). The role of the D region in inducing mixed lymphocyte reactions and its genetic linkage to the MHC (27) suggest that the human HLA-D region and the murine I region may be analogous. In addition, the tissue distribution of the HLA-D region cell-surface products (28) as well as the structure of molecules immunoprecipitated by antisera against HLA-D region (23) are very similar to those of the murine Ia alloantigens.…”

mentioning

confidence: 99%

Chemical characterization of murine Ia alloantigens determined by the i-E/i-C subregions of the H-2 complex.

Silver¹,

Russell²,

Reis³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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Alloantisera directed against the alloantigens determined b the I-E and IC subregions of the murine major histocompatibility complex precipitate two components that have molecular weights of 35,000 and 29,000. These components, when analyzed by partial NH2-terminal sequencing, show no homology to two components of similar size determined by the I-A subregion. However, the large chain determined by the murine I-E and/or IC subregion is homologous to the large chain of the human HILA-D region alloantigen, although the small chains isolated from these two species do not display any such homology. The

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“…Separate response and stimulation loci have previously been suggested in the human HL-A system (14,15). The stimulating locus (loci) of the mouse cells in our studies is situated within the H-2D region.…”

Section: Discussionmentioning

confidence: 63%

“…"Non-H-2"-associated stimulation in this MLR combination was unlikely, since the B10.A(5R) line which is congenic with the C57BL/10 line did not readily respond to the HTI cells (Table I). Hence, in the C57BL/10 plus HTI cell combinations, positive stimulation was observed with cells from two strains in which there were no differences at their H-2K, [11][12][13][14][15][16][17][18][19][20][21], and H-2S loci.…”

Section: Response To H-2d D Specificities In the Absence Of Differencmentioning

confidence: 99%

Mixed Lymphocyte Culture Responses of Mice

Plate

1974

The Journal of Experimental Medicine

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The mixed lymphocyte culture reaction (MLR) ~ through its association with the inheritance of the major histocompatibility antigens has received a great deal of interest in the field of transplantation biology (1). The recent demonstration that specific lymphoid cell types, i.e., 0-antigen-bearing thymusderived lymphocytes (T cells) with allogeneic non-P-antigen-bearing bone marrow-derived lymphocytes ("B" cells), interact to yield a proliferative response, broadens or extends the usefulness of this assay, namely to study the nature of cell-cell interactions (2). Any information, therefore, relating to the genetic control of responses in the MLR may aid in the delineation of the steps involved not only in the biological pathways of cell activation but also in Tand B-cell interaction.The responses, in vitro, to the H-2D antigenic specificities of the mouse provided us with an ideal system in which to study the genetic control of responses in mixtures of allogeneic lymphocytes (3). The data presented in this paper will demonstrate that: (a) genetic material outside of the H-2D region is involved in determining the !evel of the response to H-2D d antigens; (b) the control of the level of responsiveness was found to be a dominant trait; (c) multiple loci are involved in determining the level of MLR responses; (d) one of these loci is associated with the H-2 region though responses to H-2D d specificities were also observed, in the absence of differences between the cell donor strains at the H-2K, H-2I, and H-2S loci; and (e) responses are demonstrable even when genetic information determining MLR responsiveness is also present in the stimulating cell donor; i.e., unlike the H-2-associated specificities, differences

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Three Closely Linked Genetic Systems Relevant to Transplantation

Cited by 271 publications

References 18 publications

Synergy During in Vitro Cytotoxic Allograft Responses

Synergy During in Vitro Cytotoxic Allograft Responses

Chemical characterization of murine Ia alloantigens determined by the i-E/i-C subregions of the H-2 complex.

Mixed Lymphocyte Culture Responses of Mice

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