Mixed Lymphocyte Culture Responses of Mice

Abstract

The mixed lymphocyte culture reaction (MLR) ~ through its association with the inheritance of the major histocompatibility antigens has received a great deal of interest in the field of transplantation biology (1). The recent demonstration that specific lymphoid cell types, i.e., 0-antigen-bearing thymusderived lymphocytes (T cells) with allogeneic non-P-antigen-bearing bone marrow-derived lymphocytes ("B" cells), interact to yield a proliferative response, broadens or extends the usefulness of this assay, namely to study the nature of cell-cell interactions (2). Any information, therefore, relating to the genetic control of responses in the MLR may aid in the delineation of the steps involved not only in the biological pathways of cell activation but also in Tand B-cell interaction.The responses, in vitro, to the H-2D antigenic specificities of the mouse provided us with an ideal system in which to study the genetic control of responses in mixtures of allogeneic lymphocytes (3). The data presented in this paper will demonstrate that: (a) genetic material outside of the H-2D region is involved in determining the !evel of the response to H-2D d antigens; (b) the control of the level of responsiveness was found to be a dominant trait; (c) multiple loci are involved in determining the level of MLR responses; (d) one of these loci is associated with the H-2 region though responses to H-2D d specificities were also observed, in the absence of differences between the cell donor strains at the H-2K, H-2I, and H-2S loci; and (e) responses are demonstrable even when genetic information determining MLR responsiveness is also present in the stimulating cell donor; i.e., unlike the H-2-associated specificities, differences

Mixed Lymphocyte Reactivity and Cell‐Mediated Lympholysis to D‐End Differences of the Murine Major Histocompatibility Complex

“…In previous studies on the BlO.A-BlO,A(2R) combination, Festenstein et al (8) and Alter et al, (1) reported the absence of significant MLC or CML, although Popp did find in vivo lymphocyte transformation in this combination (18). Positive MLC response of BlO.HTG to B10.D2 has previously been reported by Plate (17). Positive CML was found in each direction between the noncongenic strains HTG and BlO,D2 by Nabholz et al , and C3H.0H to (noncongenic) BlO,D2 (15); negative data were obtained for the response of B10.D2 to BlO,D2(RlO3) (S) and a marginal or negative result for the response of ATH to A.SW (15).…”

“…The present study compares MLC and CML responses in congenic strain combinations differing at the entire MHC or with differences restricted specifically to the D region of MHC. Several previous publications have reported significant responses to isolated D-region differences (5,9,12,15,17), whereas others have reported negative results (5,8,12,15); none of these studies has quantitatively compared the strengths of stimulation by whole MHC or D region alone. An effort has therefore been made in the present work to quantitate this comparison by titrating the number of stimulating cells in culture.…”

“…At least three linked M locus (7). The influence of other genetic determinants on the magnitude of this response has also been suggested (17). Antigens coded for by genes mapping in the D and K regions of the MHC appear to be of primary importance as determinants of the induction and specificity of effector activity in CML (1).…”

Monoclonal antibodies to early pregnancy factor perturb tumour cell growth

“…After a further 16-h incubation, 20 fi] 01% trypan blue (v/v) in PBS was added to the pulsed wells and the viability of the cells determined as the percentage of eells exeluding the dye. The supernatant medium was then removed, the cells washed twice with ice-cold DMEM and precipitated with 250 /JI ice-cold 5% TCA (Plate, 1974). The precipitate was washed twice with TCA and solubilized in 0-3 ml 0 25 N NaOH; 0 25 ml of this preparation was mixed with 2 ml scintillation cocktail (Optifluor, Packard) and et/niin incorporated into acid precipitablc material were determined for eaeh well.…”

Detection of antigens specific for B-lymphoid cultured cell lines with human alloantisera.

“…These la antigens are allotypic and appear to be controlled by genes linked to H-2 (8) . The capacity to stimulate in mouse MLR is predominantly expressed by B cells (9) . Studies in mice have progressed quite rapidly due in large part to the availability of inbred strains which allow production of specific antisera detecting I-region cell membrane antigens.…”